Hecto_00030 Hecto_00030   Hecto_00050 Hecto_00050

Hecto_00040 : CDS information

close this sectionLocation

Organism
StrainJHB
Entry nameHectochlorin
Contig
Start / Stop / Direction8,248 / 14,022 / + [in whole cluster]
8,248 / 14,022 / + [in contig]
Location8248..14022 [in whole cluster]
8248..14022 [in contig]
TypeCDS
Length5,775 bp (1,924 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)HctD
GenehctD
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase; KS-AT-CM-KR-ACP
Reference
ACC
PmId
[18001088] Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. (J Nat Prod. , 2007)
comment
cyanobacterium Lyngbya majusculaのジャマイカ分離株(strain JHB)から、hectochlorin生合成gene cluster(hct)を同定。

HctD (1924aa): polyketide synthase

chlorinated hexanoate initiatorは、HctDにあるPKS module (KS-AT-CM-KR-ACP)によるketide伸長を 1回受けると提唱される。

close this sectionPKS/NRPS Module

1 malonyl-CoA
KS2..375
AT529..854
CM1116..1216
KR1522..1721
ACP1812..1889

close this sectionSequence

selected fasta
>polyketide synthase [HctD]
MDPTAIIGIGCRFPSAGNPESFWDLLQHGVHTITEVPSNRWDVDALYDPDPATPGKMNTR
WGAFLEQIDMFEPSFFGISPREAESMDPQHRLVLEVAWEALENAGVAPNKLADSQTGVFI
GIVASDYGRLVFNDPLNIDAYGGIGITPGIAANRLSYILNLHGPSLAIDTACSSSLVALH
FACQSLQSGESNLCLVGGVNLVLSPEYTLTFSQARMMASDGFCKTFDKDADGYVRGEGCG
VIVLKRLCDALRDRDNVLAVIKGSAVNHDGLSNGMTAPNGLSQQAVIRQALKNAGVAPAQ
ISYVEAHGTGTSLGDPIEVESIKAVLGQGRSPLESCGIGSIKTNIGHLEAAAGIVGLIKT
VLSLQHQQIPSHLHLKQLNPLIQLEGTPFFIPTECQPWSVAKEPRLAGVSSFSFGGTNCH
VILSEAPKQETRANEIERPAHILALSAKNQKALSELVERYEAHLKSNSSAKLADVCFTAN
TGRTHFDHRLFVTATSTTEMQKALRDLAEGKDTVPTGQICSPKRQKIAFLFTGQGSVYVN
MGRQLYDTQPTFRNCLKRCEEILRPYLEVPLLSVLYPDIDLSSPTSGEANQKLYNTAYTQ
PALFALEYALCQLWKSWGIEADAVIGHSVGEYVAACVAGVFSLEDGLKLIAMRGQLMQQL
PPGGEMLSLLASESQVREVIAPYGEQVAIAALNGPSSIVISGKSEAIQEIYSKVSTQGIK
AKFLQVSHAFHSPLMEPMLGQFEVAAWQISYHKPQIPLISNVTGQLIGDDTSTPEYWVNH
IRQPVRFADGMQTLYQEGCQIFLEIGPQAILLGMGRQCLPEDRGVWLPSLRKDREDWQQL
LESLGELYVRGLEVDWSGLDKDYTREKVVLPTYPFQRERYWTKATNFTNFVDQEEFQDWL
YEVEWQPQASFGGQFPSEELLDPLTINQKLQPLVSSLASVNNLDSYSQLVRQLETLSVEY
VLQAFDEMGWSLKLGENFSTESLAQKLGVVPQYQQLFNLLLEMLAEVEILRHTENQWVTL
QTPEKVNLQQKNQTLLAQFPSANAELTMLERCGSQLRAVLQGTIDPLQVLFPEGDFTIAT
QLYEQSPEAQFTNTLVQRAIATALENLPPSRGVRLLEIGAGTGGTTSYILPHLNPEQTEY
IFTDIGTSFTSKAQERFQDYPFIHYQNLDIEKNPELQGFEPHQYDVIVAANVIHATSSLR
KTLQHVRQLLKPGGMFVLLEITTPRRWVTLSFGMLEGWWKFSDFDLRPNQPLLSPSKWQQ
LLVESGFPKVVNLPDVRGTEEVLSSRSLIIAQTAVTPSLSPSEGWLIFADRQGIAQQLAD
RLRTFGQVCNLVFPGKEYQQIASDEFTINPENSDDYLKLVTEVKANLPDCKGVFHLWSLD
TPRAEELTPEGLKVASKEGCGSALYLVQALTKAQFLQSPRLWLVTKGAQPVLPIKDSISG
LAQSPLWGMGRVISLEHPELSVRMLDLDPNVAPEHSCTTLLTEIGDANDENHIVFRDAQR
YVARLINKKFKESQSFQFRPDATYLITGGTGFLGLKLAQWMVEQGACQLVLIGRKGLPQR
EDWPNLDRESNEWKQVQAIQLMEEKGARVTVYASDVTDQAQMSSIIKGIDTAQKPLRGII
HAAGLSGEHKLSQMNYKVFESLLSPKTIGTWVLHQLTKEISLDFFICFSSIASVWGSLGH
GHYDAANHFLNILASYRRSIGLPALSINWGIFVVGGMIADENYVRWMNQIGIEALQPEEG
FNALKFLLKTNAIQPLVTKVDWSTFKEYYEVRGLGKLFSDLMTVSTVDQTTPSIETIKSQ
ESSILEQLKAASPSKIENILLTHFQNEIAKSLRTSASEISLQKPLNSMGLDSLMAVELRN
MSLVKLGIDIPTVKFIEGLSIADIVTEVKDQLNQIDASQKVEWKNSQQLYTEEPDQIERV
RGEL
selected fasta
>polyketide synthase [HctD]
ATGGATCCAACCGCGATTATAGGTATTGGTTGTCGTTTTCCAAGTGCTGGAAATCCAGAA
AGCTTTTGGGATTTGCTGCAGCATGGGGTGCATACCATCACTGAAGTTCCGTCAAATCGA
TGGGATGTTGATGCCTTGTATGATCCCGATCCAGCTACACCAGGAAAAATGAACACCCGT
TGGGGCGCTTTTCTGGAACAGATAGATATGTTTGAACCGAGTTTCTTCGGAATTTCTCCC
CGAGAAGCAGAGTCAATGGATCCCCAGCATAGACTAGTCTTAGAGGTAGCCTGGGAAGCT
CTGGAAAATGCAGGAGTGGCACCAAATAAGTTAGCTGACAGCCAAACAGGTGTGTTTATC
GGAATCGTTGCTAGTGATTATGGCCGACTGGTGTTCAATGATCCCCTTAATATAGATGCT
TATGGGGGCATTGGCATCACGCCTGGTATTGCAGCGAATCGTCTCTCCTATATACTGAAT
TTGCACGGACCTAGCTTGGCGATAGACACAGCTTGCTCGTCATCGCTGGTAGCATTGCAT
TTTGCCTGTCAAAGCCTGCAAAGCGGTGAGTCTAATTTGTGCTTGGTCGGAGGGGTGAAT
TTGGTTTTGTCCCCCGAATACACTCTCACTTTTTCTCAAGCTCGGATGATGGCGTCGGAC
GGTTTTTGTAAGACTTTTGACAAAGACGCTGATGGTTATGTACGAGGGGAAGGATGTGGT
GTAATTGTCCTCAAACGCCTCTGTGATGCCCTGCGGGATAGAGACAATGTTTTAGCAGTT
ATCAAAGGCTCGGCAGTTAACCATGATGGTCTAAGCAATGGCATGACTGCACCCAATGGG
CTTTCCCAGCAGGCAGTTATCAGGCAAGCATTAAAAAATGCTGGTGTAGCGCCAGCCCAG
ATTAGTTATGTTGAAGCTCACGGTACTGGCACTTCCTTGGGGGACCCAATAGAAGTTGAA
TCCATAAAAGCAGTACTGGGTCAAGGGCGATCGCCTCTTGAGTCCTGTGGGATTGGTTCA
ATTAAGACCAATATCGGTCATTTGGAAGCTGCGGCGGGAATAGTTGGTCTAATTAAGACA
GTTCTATCACTGCAACATCAGCAAATACCATCCCACCTGCACCTAAAACAACTGAATCCC
TTAATTCAGTTAGAAGGTACACCCTTTTTTATACCTACTGAGTGCCAACCTTGGTCTGTT
GCTAAAGAGCCTCGCTTGGCTGGGGTCAGTTCCTTTAGCTTTGGTGGCACAAATTGCCAT
GTCATCTTGTCGGAAGCTCCCAAACAAGAAACCAGAGCTAATGAAATTGAACGCCCTGCT
CACATTCTTGCTCTCTCAGCCAAAAATCAGAAGGCTTTGAGTGAATTAGTAGAGCGCTAT
GAGGCTCATCTGAAATCAAACTCATCCGCAAAGCTGGCAGACGTTTGTTTCACTGCTAAT
ACAGGAAGAACGCATTTTGATCACCGCCTTTTTGTTACGGCAACATCTACAACAGAGATG
CAAAAGGCTCTGCGCGACCTTGCGGAGGGCAAAGATACAGTGCCAACCGGTCAAATATGC
AGTCCCAAGCGCCAGAAAATAGCGTTCTTATTTACTGGTCAAGGGTCTGTGTATGTGAAT
ATGGGGCGTCAACTCTACGATACCCAACCGACATTTCGTAATTGCCTCAAACGCTGTGAA
GAAATTTTACGTCCCTATCTAGAAGTTCCACTACTCTCTGTTCTCTATCCAGATATTGAT
TTGTCTTCTCCAACATCTGGCGAGGCAAACCAAAAATTATACAACACGGCCTATACTCAA
CCTGCTTTATTCGCTCTAGAATATGCCCTATGCCAATTATGGAAATCTTGGGGCATAGAG
GCGGATGCTGTAATCGGTCATAGTGTGGGGGAATATGTCGCTGCCTGTGTTGCTGGGGTT
TTTAGCCTGGAAGACGGTCTCAAATTGATTGCCATGCGGGGACAATTAATGCAACAATTG
CCTCCAGGTGGTGAGATGTTGTCCTTGCTCGCATCAGAATCTCAAGTGCGAGAAGTGATC
GCTCCTTATGGCGAGCAAGTTGCGATCGCAGCTCTCAATGGCCCCAGCAGTATAGTAATT
TCTGGTAAAAGTGAAGCCATCCAGGAGATTTACAGCAAGGTGTCAACCCAAGGAATAAAA
GCAAAATTTTTGCAAGTATCCCATGCTTTTCACTCTCCGCTGATGGAACCAATGCTAGGG
CAGTTTGAGGTAGCAGCATGGCAAATCTCCTACCATAAGCCTCAGATTCCTCTAATTTCT
AATGTTACTGGGCAACTAATAGGCGATGACACTAGCACTCCTGAGTATTGGGTGAATCAT
ATACGACAACCAGTGCGCTTTGCTGATGGTATGCAGACTTTATACCAGGAAGGCTGTCAA
ATTTTCTTGGAAATCGGCCCCCAGGCAATTTTGCTGGGAATGGGACGCCAATGCTTACCA
GAGGACAGGGGGGTTTGGTTGCCTTCTCTGCGCAAAGATCGCGAAGACTGGCAACAATTG
CTAGAGAGCTTAGGGGAATTGTACGTAAGAGGTCTAGAGGTAGACTGGTCAGGCCTTGAC
AAAGATTATACCCGTGAGAAAGTAGTATTACCTACTTATCCTTTCCAAAGAGAACGGTAT
TGGACTAAAGCAACTAACTTTACTAACTTTGTAGATCAAGAGGAGTTCCAGGATTGGTTA
TATGAAGTAGAATGGCAACCTCAAGCTAGTTTTGGCGGCCAGTTTCCATCAGAGGAACTT
TTAGACCCATTGACCATTAACCAGAAACTACAACCTTTGGTGTCGTCTTTAGCCTCTGTT
AATAACTTGGATAGCTACAGTCAACTTGTGAGACAACTAGAAACTTTGAGTGTGGAATAT
GTCTTGCAAGCCTTTGACGAAATGGGATGGTCATTAAAATTGGGGGAAAACTTCTCCACT
GAATCCCTAGCCCAAAAATTAGGTGTAGTACCACAATATCAACAACTTTTCAACCTGTTG
TTAGAGATGCTAGCAGAAGTAGAAATTCTGCGGCACACCGAAAATCAGTGGGTGACACTC
CAAACTCCTGAAAAAGTTAATCTACAACAAAAAAATCAGACCTTACTGGCTCAATTCCCC
AGCGCTAATGCAGAACTAACTATGTTAGAGCGTTGTGGTTCCCAATTGAGAGCAGTTTTG
CAGGGAACTATAGATCCCCTGCAAGTTCTCTTCCCTGAAGGGGATTTTACTATAGCTACT
CAACTATATGAACAGTCTCCTGAAGCACAATTCACCAACACTTTAGTCCAGCGTGCGATC
GCCACCGCTCTGGAAAATTTACCTCCAAGCCGAGGGGTGCGATTATTAGAAATTGGAGCA
GGGACGGGGGGAACTACTAGCTATATATTGCCTCATCTGAATCCAGAGCAAACAGAATAT
ATCTTCACCGATATCGGTACTTCCTTTACTAGCAAAGCACAGGAGAGATTTCAGGATTAC
CCATTCATACACTATCAAAACTTAGACATTGAAAAAAATCCCGAACTTCAAGGGTTTGAA
CCCCATCAGTACGATGTGATAGTAGCAGCTAACGTAATCCATGCTACATCCTCTTTGCGT
AAAACCCTACAACATGTGAGACAGTTGTTGAAACCTGGAGGGATGTTTGTGTTGTTGGAA
ATCACAACTCCCAGGCGGTGGGTGACTTTATCCTTTGGAATGTTAGAGGGATGGTGGAAG
TTTAGCGATTTTGATTTACGTCCCAATCAGCCCCTACTAAGTCCATCAAAGTGGCAGCAA
TTGTTAGTCGAAAGCGGTTTCCCAAAAGTGGTAAACCTGCCCGATGTTCGGGGAACAGAA
GAGGTGCTATCCTCCCGGAGTCTAATTATTGCTCAAACTGCAGTGACTCCATCGTTGTCG
CCCAGTGAAGGATGGTTAATTTTTGCAGACCGACAAGGTATCGCTCAACAGTTGGCAGAT
CGACTGCGAACCTTCGGACAAGTCTGTAATTTGGTGTTTCCAGGAAAGGAATATCAACAG
ATAGCATCAGATGAGTTTACCATAAACCCGGAAAATTCTGATGACTACTTAAAACTGGTG
ACAGAGGTAAAAGCTAATCTGCCCGATTGTAAAGGAGTGTTCCATCTATGGAGCTTAGAT
ACTCCAAGAGCAGAGGAACTAACACCAGAAGGCTTGAAAGTAGCTTCAAAAGAGGGATGT
GGGAGTGCCTTATACTTAGTTCAAGCCTTGACCAAAGCACAATTTTTGCAATCTCCCCGC
CTGTGGTTGGTGACCAAAGGTGCCCAGCCAGTTCTTCCCATCAAAGATTCAATATCAGGG
TTAGCTCAATCACCTTTGTGGGGAATGGGTAGAGTAATATCTTTGGAACATCCCGAATTG
TCAGTCCGAATGCTAGATTTAGACCCTAATGTAGCACCAGAGCATTCCTGTACAACACTT
TTAACAGAAATCGGAGATGCAAATGATGAAAACCATATTGTTTTCCGAGATGCACAGCGT
TACGTAGCCCGTCTAATAAATAAGAAGTTTAAAGAATCTCAAAGTTTTCAGTTCCGGCCT
GATGCCACTTACTTAATTACTGGCGGTACTGGTTTCTTAGGATTGAAGTTAGCCCAGTGG
ATGGTAGAGCAAGGAGCTTGCCAGTTGGTGCTAATTGGACGTAAAGGGCTGCCCCAACGG
GAAGATTGGCCAAACCTGGATCGAGAGAGTAATGAATGGAAACAGGTTCAAGCTATTCAA
CTCATGGAAGAAAAGGGAGCTAGGGTAACAGTGTACGCATCTGATGTTACCGACCAAGCT
CAAATGTCCTCAATTATTAAGGGGATCGATACTGCTCAAAAACCACTACGGGGTATTATT
CATGCGGCTGGCTTAAGTGGCGAACACAAATTAAGTCAGATGAATTATAAAGTATTTGAA
TCTTTGCTTTCCCCTAAGACAATTGGAACCTGGGTTCTACATCAACTAACTAAGGAGATT
AGTTTAGACTTTTTTATCTGCTTTTCTTCAATTGCGTCAGTGTGGGGTTCTCTAGGTCAC
GGACATTATGATGCAGCTAACCACTTTCTCAATATCCTAGCCTCTTATCGTCGTTCTATT
GGGCTTCCAGCGTTAAGTATAAATTGGGGAATTTTCGTTGTTGGGGGAATGATTGCTGAC
GAAAATTATGTCCGATGGATGAACCAGATTGGGATAGAAGCATTACAACCAGAGGAAGGA
TTCAATGCCTTAAAATTCCTTTTGAAAACCAATGCCATTCAACCGCTAGTGACAAAGGTA
GATTGGTCTACATTTAAGGAATATTATGAAGTAAGGGGATTAGGAAAGCTATTTAGTGAC
CTGATGACTGTATCTACTGTGGATCAGACAACCCCTTCAATAGAGACAATAAAGAGTCAG
GAGTCTAGCATTTTAGAACAATTAAAAGCAGCTTCTCCAAGTAAAATAGAAAACATTTTA
TTGACTCATTTTCAAAATGAAATTGCGAAATCCTTACGGACAAGCGCTTCAGAAATAAGC
TTACAAAAACCTTTAAATAGCATGGGACTTGATTCCCTAATGGCTGTAGAATTGCGAAAT
ATGAGTCTAGTGAAGTTAGGGATAGATATTCCTACTGTAAAATTTATAGAAGGGCTTAGT
ATTGCTGATATCGTAACTGAAGTAAAAGACCAACTGAACCAGATTGACGCTAGTCAAAAA
GTTGAGTGGAAAAATTCCCAACAACTTTACACAGAAGAACCAGACCAGATAGAACGAGTA
AGGGGTGAATTATGA
[1] KS2..375
[1] AT529..854
[1] malonyl-CoA728..732
[1] CM1116..1216
[1] KR1522..1721
[1] ACP1812..1889
[1] KS4..1125
[1] AT1585..2562
[1] malonyl-CoA2182..2196
[1] CM3346..3648
[1] KR4564..5163
[1] ACP5434..5667

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[523-660] 2.70000000000002e-89 G3DSA:3.40.366.10 [728-843] 2.70000000000002e-89 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR009081 Acyl carrier protein-like (Domain)
[1816-1891] 5.29999657550189e-11 SSF47336
SSF47336   ACP_like
[1828-1895] 1.7e-09 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1812-1889] PS50075
PS50075   ACP_DOMAIN
[1826-1887] 3.8e-07 PF00550
PF00550   PP-binding
IPR013217 Methyltransferase type 12 (Domain)
[1116-1216] 2.6e-22 PF08242
PF08242   Methyltransf_12
IPR013968 Polyketide synthase, KR (Domain)
[1522-1711] 3.2e-60 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[2-250] 2.20000000000002e-93 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[258-375] 1.40000000000001e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[529-854] 5.09999999999993e-66 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[526-837] 1.90000694315261e-76 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[662-727] 1e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[5-262] 1.69999999999998e-99 G3DSA:3.40.47.10 [263-430] 6.10000000000004e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[1-374] 1.50000965748778e-98 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1522-1750] 2.4e-39 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[163-179] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[530-834] SM00827
SM00827   PKS_AT
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[4-428] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1522-1721] 1.20000117458134e-43 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Hecto_00030 Hecto_00030   Hecto_00050 Hecto_00050
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