Hecto_00070 : CDS information

Location

Organism	Lyngbya majuscula
Strain	JHB
Entry name	Hectochlorin
Contig	AY974560
Start / Stop / Direction	36,589 / 37,932 / + [in whole cluster] 36,589 / 37,932 / + [in contig]
Location	36589..37932 [in whole cluster] 36589..37932 [in contig]
Type	CDS
Length	1,344 bp (447 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	HctG
Gene	hctG
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	cytochrome P450 monooxygenase
Reference	ACC Q1EDA9 PmId [18001088] Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. (J Nat Prod. , 2007) comment cyanobacterium Lyngbya majusculaのジャマイカ分離株(strain JHB)から、hectochlorin生合成gene cluster(hct)を同定。 HctG (447aa): cytochrome P450, monooxygenase 配列解析のみ。 HctGとHctHはお互いによく似ている。 NRPS module 2とmodule 4では、2-oxo-isovaleric acid (O-IVA)がA domainによって活性化された後にKR domainでD-2-hydroxyisovaleric acid (D-2-HIV)へと還元され、さらにHctG or HctH で酸化されて2,3-dihydroxyisovaleric acid (DHIV)を形成すると提唱されている。
Family/Domain	FD IPR001128 comment [IPR001128] Cytochrome P450 (Family)

Sequence

>putative cytochrome P450 [HctG]
MKLPPGPQTPAILQMIEWVFHPSKLMQTCAQRYGDTFTVKVGPNLAPVVFLSDPKAIQKI
FTADLKQFETGKLNKIAQPLIGEYSLMLQDGKYHQRQRRLLMPPFHGERMRRYNKIIDDI
AKQVISQWPEGKPFSIRPSIGKISLELIVECVFGLRKGPRSQHLIQLLTLMAEYTSKPLA
IALVSCKPMQRDFGSWSPWGSFIRLKQQITKLIYDEIGERRSQFDSSRTDILTLLMSARD
EDGMPMTDVELHDQLITFLMGGHDTTTTALVWAFYWIHYLPEVREKLLRELDSLGDDLEE
SNIIKLRYLNAVCSETLRINPISVLTLFRSVKSPLQLMSYQYDPGTILAGCIYLTHRRED
LYPEPEKFKPERFLERQFSFYEYLPFGGGNRRCVGDALAMFVMKRVIATVLREKQLTLVD
RRPVKPVFRGPGLAPSGSMQMVAVTKK

>putative cytochrome P450 [HctG]
ATGAAACTACCTCCAGGTCCTCAGACTCCTGCCATTCTACAGATGATTGAGTGGGTGTTT
CATCCGTCAAAACTCATGCAAACCTGTGCCCAGCGTTATGGGGATACCTTTACAGTAAAG
GTTGGTCCGAATCTTGCTCCTGTAGTATTTTTGAGCGATCCGAAAGCAATTCAGAAAATT
TTTACTGCTGATTTAAAGCAATTTGAGACTGGAAAACTAAACAAGATAGCTCAACCTTTG
ATAGGGGAATACTCACTAATGCTCCAGGATGGCAAATATCACCAACGTCAAAGACGATTG
TTAATGCCTCCTTTTCATGGAGAACGCATGCGGAGATATAATAAAATAATTGATGATATC
GCCAAACAAGTCATAAGCCAATGGCCAGAGGGTAAACCCTTCTCAATCCGTCCATCGATA
GGAAAAATTTCCTTAGAGTTGATTGTCGAATGTGTATTTGGTCTCAGGAAGGGACCGCGT
TCTCAACATCTAATACAGCTTCTCACTTTAATGGCTGAATATACTAGCAAGCCCCTAGCT
ATCGCTTTGGTTAGTTGCAAGCCAATGCAACGGGATTTCGGTTCTTGGAGTCCGTGGGGA
AGTTTTATACGCTTGAAGCAGCAAATTACGAAACTAATCTACGACGAGATTGGAGAACGC
CGGTCTCAATTTGACTCGTCACGTACCGACATCCTCACCTTGCTGATGTCTGCTCGCGAT
GAAGATGGTATGCCAATGACAGATGTAGAGTTACACGATCAGTTAATTACGTTCCTAATG
GGGGGACACGATACTACCACTACGGCTTTAGTATGGGCTTTTTACTGGATTCACTATCTA
CCAGAGGTACGTGAAAAGCTTCTACGAGAACTCGATAGCCTAGGTGATGACCTAGAAGAA
TCTAATATTATCAAACTTCGCTATCTCAATGCTGTATGTTCGGAAACCTTGCGAATCAAC
CCCATTTCTGTTCTTACCTTATTTCGAAGTGTGAAATCTCCATTGCAGTTGATGAGCTAC
CAGTACGATCCCGGTACAATCCTAGCGGGCTGTATTTACTTGACACACCGTAGGGAGGAT
TTATATCCCGAGCCAGAAAAATTTAAGCCAGAGCGTTTTCTGGAGCGGCAATTTTCATTT
TACGAATATTTGCCGTTTGGGGGCGGAAATCGTCGCTGTGTGGGAGATGCGTTGGCTATG
TTTGTAATGAAACGAGTAATTGCTACAGTTCTGCGCGAAAAACAGCTAACGCTTGTCGAT
CGTCGTCCTGTCAAACCTGTATTCCGAGGGCCTGGTTTAGCACCTTCTGGAAGTATGCAG
ATGGTTGCTGTTACCAAAAAATAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 PF00067 p450 IPR002401 Cytochrome P450, E-class, group I (Family) PR00463 EP450I IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Hecto_00060 Hecto_00080