Heda_00010 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	6,378 / 1 / - [in whole cluster] 6,378 / 1 / - [in contig]
Location	complement(1..6378) [in whole cluster] complement(1..6378) [in contig]
Type	CDS
Length	6,378 bp (2,125 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	type I PKS
Gene	hedU
Gene (GenBank)
EC number
Keyword	starter unit
Note
Note (GenBank)	orf32
Reference	ACC Q67G57 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) [19942143] In vivo and in vitro analysis of the hedamycin polyketide synthase. (Chem Biol. , 2009) comment [PMID: 15271354](2004) hedamycinの生合成gene clusterの報告。 hedU: Type I PKS KS (48-471), AT(574-888), DH (918-1035), KR(1378-1557), ACP (1652-1712),KS (1766-2124) AT domainはmalonyl-CoA transferのために必要な保存motifを含む。 --- [PMID: 19942143](2009) HedS or HedFが鎖合成の開始、伸長に関係しないことがわかったので、HedT and HedUによってコードされるtype I PKSがhedamycin aglyconeの2,4-hexadienyl primer unit合成を担い、それでKS-CLFを直接的にprimeするという生合成モデルを検証。 type I PKS HedT, HedUは発現、精製に失敗。なのでHedUのC末ACP domainを発現、精製、Sfpでholo-ACPへ変換。 [14C]-2,4-hexadienyl CoAの代わりに代理primer unitとして[14C]-hexanoyl CoAを使用。 [14C]-hexanoyl-HedU ACPはhed KS-CLFへacyl鎖を効率的に移動できた。

PKS/NRPS Module

1		malonyl-CoA
2

KS	48..422

AT	574..886

DH	931..1091

KR	1380..1560

ACP	1664..1734

KS	1766..2124

Sequence

>polyketide synthase [type I PKS]
MIFEHPTPRALADHLAELLHPADRDRPAAPALLTSPPAPSDTTDPDNDAIAIIGIGCRFP
GGITTPEQLWQLLTEERHAITDFPTNRGWPDNLHHPDPTHPGTTYTRKGGFLHDADQFDA
HFFGISPREALAMDPQQRSLLETAWHTLEHAGIDPTTLHGTDTGVFVGASPLEYGPRMQD
APPSVQGHVMTGVSPSILSGRLAYQFGFTGPALTLDTACSSSLVALHTATQALRNNECTL
ALVGGTTIMATPGTFIEFSRLQGLSPDGHCRSFSAHANGTGWSEGTALLLLEPLTQAQRN
NHTVHAVIRATATNQDGPSNGLTAPNGQAQQRLIHHTLTTAHLTPHDIDAIEAHGTATPL
GDPIEARALMETYGRDRPADRPVYLGSVKSNLGHTQAAAGAAGVIKMIQALHHNLLPKTL
HTDQPTPHIDWNNTPLQLLTQPQPWPHNPHRPRRAAISSFGISGTNAHLILEEPPTTPTP
QQPTTLPITPYILTAKTPTALHTHAHHLTQHPTHHHPTHTAHTLATRTHHPHRAVILTPD
PTTLTTTLHHLTTNQPHPHLTTGSVTGDGRLGLLFPGQGAQQTGAGRELYDAFPGFAEAL
DAVLEELDRHLDRPLRSVMWGEDDELLNLTQYAQPALFALEVALFRLVESWGIEPDVLIG
HSVGELAAAHAAGILTLGDACALVAARGRLMGALPEDGAMLAVEASEEELAPLLGDEVDL
AAVNGPTSLVVSGTASAVDCLAARWTARGRRATRLRVSHAFHSRLMEPMLARFAEVAEGL
AYGAPRIPVVSTLTGAVVTDEAMSGASYWVRHARETVRFADAVRTTADRGVGALLELGPR
GTLSGMVPDCLPEPDVMIAVPALPENRTEAAALLSSVAQVFVRGTDVDWTRLFHGTGARH
VDLPLYPFERQRYWLTAPRATAATGLAATSHPFLGATTELAADRTTLFSGRVSVQDHPWL
ADHRIDGVVLLPGTALLELALHAGRHTGCPHIEELTLTAPVVLGEEDARAVQLLVGAPDS
AGRRSLTVHTRPQDGDEPWTTHAAAVLAPNGPSASVDPVELPPDAEDVAFEDLYSRCASA
GYGYGPAFRRVHRLRRHGDDVHAELSPPTGAGFHLHPALLDAALHPLLPTDAGDTLLPFA
FSGVTLHDTPPVSGAVHVHARLAGLDDQGTRASLVLTGPDGTPFASVHDVVLRPARPLDA
GLHTVAWAAAGERTAAPDTTAWALVTADGTAPAPLPLAHTVHSDLATVPTDAPATVVLPL
RADPRAPHALLARVQQWLTGPHHPDGRLVVVTGGPADDRAGVHQAAACGLLLSAISEHPG
RFVLADLDEEPSSWAALPEAVATGEPRLRIRSGQVSVPRLAPVPHAGSKAAASPVFSPEG
TVLVTGGTGRLGALLARHLVARHGVRHLLLVSRRGADAPGAAELTAELRDAGARVTVRAC
DAADREALAAVLEDVPAPHPLTAVIHTAGVLDDCVVERLTPGRLDAVLRPKADAAWHLHE
LTRDLGLRAFVLFSSVSGLIGTAGQANYAAANAFLDALAGHRRRLALPATSLAWGLWAGD
GGMAGALGAQDIARFAREGIAPLEPEEALRLFDAALAHEEALLAPVRVDRAALRRRARSG
ADISPLMREAAGGTPRACEHDGSGAVPLSGRLAGRSEAERRRVLSDVVHTQAAAVLGFAD
PSGIVSERAFKELGFDSLTSVELRNRLNSATGLRLSAGVIFEHPTPRALADHLAELLHPA
DRDRPAAPALLTSPPAPSDTTDPDNDAIAIIGIGCRFPGGITTPEQLWQLLTEERHAITD
FPTNRGWPDNLHHPDPTHPGTTYTRKGGFLHDADQFDAHFFGISPREALAMDPQQRSLLE
TAWHTLEHAGIDPTTLHGTDTGVFVGMMYHDYAPAAGQMPRDLEGLLLTGNLGSVLSGRL
AYQFGFTGPALTLDTACSSSLVALHTATQALRNNECTLALAGGTTIMATPGTFIEFSRLQ
GLSPDGHCRSFSAHANGTGWSEGTALLLLEPLTQAQRNNHTVHAVIRATATNQDGPSNGL
TAPNGQAQQRLIHHTLTTAHLTPHDIDAIEAHGTATPLGDPIEVQALHTVYGQDRTRPLW
LGSLKSNLGHTQAAAGAAGVIKMIH

>polyketide synthase [type I PKS]
GTGATCTTCGAGCATCCGACGCCGCGGGCGCTGGCCGACCACCTCGCGGAACTGCTGCAC
CCCGCAGACCGGGACCGGCCCGCCGCCCCCGCACTCCTCACCTCACCCCCGGCGCCCTCC
GACACCACCGACCCCGACAACGACGCCATCGCCATCATCGGCATCGGCTGCCGCTTCCCC
GGCGGAATCACCACCCCCGAACAACTCTGGCAACTCCTCACCGAAGAACGCCACGCCATC
ACCGACTTCCCCACCAACCGCGGCTGGCCCGACAACCTCCACCACCCCGACCCCACCCAC
CCCGGCACCACCTACACCCGCAAAGGCGGCTTCCTCCACGACGCCGACCAGTTCGACGCC
CACTTCTTCGGCATCTCCCCCCGCGAAGCCCTCGCCATGGACCCCCAGCAACGCAGCCTC
CTCGAAACCGCCTGGCACACCCTCGAACACGCCGGCATCGACCCCACCACCCTCCACGGC
ACCGACACCGGCGTCTTCGTCGGGGCATCGCCGCTGGAGTACGGGCCGCGGATGCAGGAC
GCGCCGCCCTCGGTCCAGGGTCACGTGATGACGGGTGTGTCGCCGAGCATCCTGTCGGGG
CGGCTGGCGTACCAGTTCGGGTTCACCGGCCCCGCGCTCACCCTCGACACCGCCTGCTCC
TCCTCCCTCGTCGCCCTCCACACCGCCACCCAAGCCCTCCGCAACAACGAATGCACCCTC
GCCCTCGTCGGCGGCACCACCATCATGGCCACCCCAGGCACCTTCATCGAATTCAGCCGC
CTCCAAGGACTCTCCCCCGACGGACACTGCCGCTCCTTCTCCGCCCACGCCAACGGCACC
GGCTGGTCCGAAGGAACAGCCCTCCTCCTCCTCGAACCCCTCACCCAAGCACAACGCAAC
AACCACACCGTCCACGCCGTCATCCGCGCCACCGCCACCAACCAGGACGGCCCCTCCAAC
GGCCTCACCGCCCCCAACGGCCAAGCCCAACAACGCCTCATCCACCACACCCTCACCACC
GCCCACCTCACCCCCCACGACATCGACGCCATCGAAGCCCACGGCACCGCCACCCCCCTC
GGCGACCCCATCGAGGCCCGGGCCCTGATGGAGACCTACGGCCGGGACCGGCCCGCCGAC
CGGCCGGTGTACCTGGGCTCGGTCAAGTCCAATCTCGGGCACACCCAAGCCGCCGCCGGA
GCCGCCGGCGTCATCAAAATGATCCAAGCCCTCCACCACAACCTCCTCCCCAAAACCCTC
CACACCGACCAACCCACCCCCCACATCGACTGGAACAACACCCCCCTCCAACTCCTCACC
CAACCCCAACCCTGGCCCCACAACCCCCACCGACCCCGCCGCGCAGCCATCTCCTCCTTC
GGCATCAGCGGCACCAACGCCCACCTCATCCTCGAAGAACCCCCCACCACCCCCACCCCC
CAACAACCCACCACCCTCCCCATCACCCCCTACATCCTCACCGCCAAGACCCCCACCGCC
CTCCACACCCACGCACACCACCTCACCCAACACCCCACCCACCACCACCCCACCCACACC
GCCCACACCCTCGCCACCCGCACCCACCACCCCCACCGCGCCGTCATCCTCACCCCCGAC
CCCACCACCCTCACCACCACCCTCCACCACCTCACCACCAACCAACCCCACCCCCACCTC
ACCACCGGCAGCGTCACCGGCGACGGCAGGCTCGGTCTGCTCTTCCCCGGCCAGGGCGCC
CAGCAGACGGGCGCCGGACGTGAGCTCTACGACGCGTTCCCGGGTTTCGCCGAAGCGCTC
GACGCCGTCCTGGAGGAGCTGGACCGGCACCTGGACCGGCCCCTGCGCTCGGTGATGTGG
GGCGAGGACGACGAGTTGTTGAACCTCACTCAGTACGCGCAGCCGGCCCTCTTCGCCCTG
GAGGTGGCCCTGTTCCGCCTCGTGGAGTCGTGGGGGATCGAGCCGGACGTGCTGATCGGC
CACTCGGTGGGCGAGTTGGCCGCGGCCCACGCCGCCGGCATCCTCACCCTGGGTGACGCC
TGCGCCCTGGTCGCCGCCCGAGGACGGCTGATGGGCGCCCTTCCGGAAGACGGCGCGATG
CTGGCGGTGGAGGCGTCCGAGGAGGAACTGGCGCCGCTGCTGGGCGACGAGGTGGACCTC
GCCGCGGTCAACGGCCCCACGTCGCTGGTGGTGTCCGGCACCGCCTCGGCGGTCGACTGT
CTCGCCGCACGCTGGACGGCACGCGGGCGCAGGGCCACCCGGCTGCGGGTCAGCCATGCC
TTCCACTCACGGCTGATGGAGCCGATGCTGGCCCGGTTCGCCGAGGTGGCGGAAGGGCTG
GCCTACGGCGCTCCGCGGATACCGGTGGTGTCCACCCTGACGGGCGCGGTGGTGACGGAC
GAGGCGATGTCCGGCGCCTCGTACTGGGTGCGGCACGCGCGGGAGACGGTCCGCTTCGCA
GACGCGGTGCGGACCACGGCGGACCGCGGTGTCGGCGCGCTGCTGGAGCTGGGGCCACGG
GGGACGCTGTCGGGCATGGTGCCGGACTGCCTTCCGGAGCCGGACGTGATGATCGCGGTG
CCCGCGCTGCCCGAAAACCGGACGGAGGCGGCGGCCCTGCTGTCGTCCGTGGCGCAGGTG
TTCGTCCGCGGCACCGACGTGGACTGGACGCGCCTGTTCCACGGCACCGGCGCCCGCCAC
GTCGACCTGCCCCTCTACCCCTTCGAACGACAGCGGTACTGGCTGACGGCGCCCCGCGCC
ACGGCCGCCACGGGTCTGGCCGCCACCTCGCACCCCTTCCTCGGCGCGACCACCGAACTC
GCCGCCGACCGCACGACGTTGTTCTCCGGGCGGGTCAGTGTCCAGGACCACCCGTGGCTG
GCCGACCACCGGATCGACGGCGTCGTCCTGCTGCCGGGCACGGCCCTGCTGGAGCTGGCC
CTGCACGCCGGACGGCACACCGGCTGCCCGCACATCGAGGAGCTGACCCTCACCGCCCCG
GTCGTCCTGGGCGAGGAGGACGCCCGCGCAGTCCAACTGCTCGTCGGCGCACCGGACTCC
GCGGGACGCCGCTCCCTGACCGTCCACACCCGGCCGCAGGACGGGGACGAGCCGTGGACC
ACGCACGCCGCCGCGGTCCTCGCCCCCAACGGCCCGTCCGCGTCCGTTGACCCCGTCGAA
CTGCCCCCGGACGCCGAGGACGTGGCGTTCGAGGACCTCTACAGTCGCTGTGCGTCCGCC
GGGTACGGCTACGGTCCCGCCTTCCGCCGGGTCCACCGGCTCCGCCGGCACGGGGACGAC
GTCCACGCCGAGCTGTCGCCGCCCACCGGCGCCGGCTTCCACCTCCACCCGGCCCTCCTG
GACGCCGCGCTGCACCCGCTGCTGCCCACCGACGCCGGCGACACCCTGCTGCCGTTCGCC
TTCTCGGGCGTCACCCTGCACGACACGCCGCCCGTCTCCGGCGCCGTGCACGTCCACGCC
CGGCTCGCCGGACTGGACGACCAGGGCACCCGTGCCTCACTCGTGCTGACCGGCCCCGAC
GGGACCCCGTTCGCCTCGGTCCACGATGTCGTCCTGCGCCCGGCACGTCCCCTCGACGCC
GGGCTCCACACCGTGGCCTGGGCTGCTGCCGGGGAGCGGACGGCCGCGCCGGACACCACC
GCCTGGGCGCTCGTCACCGCCGACGGGACGGCGCCCGCGCCGCTCCCCCTGGCCCACACC
GTCCATTCCGACCTCGCTACCGTGCCCACCGATGCCCCCGCCACGGTGGTGCTGCCCCTG
AGGGCGGACCCCCGCGCGCCGCACGCGCTCCTCGCCCGCGTCCAGCAGTGGCTCACCGGC
CCGCACCACCCGGACGGCCGACTGGTGGTGGTCACCGGCGGGCCGGCCGACGACCGGGCC
GGGGTGCACCAGGCGGCGGCGTGCGGTCTGCTGTTGTCGGCGATCTCCGAGCACCCGGGG
CGCTTCGTCCTGGCCGACCTCGACGAGGAGCCGTCGTCCTGGGCGGCTCTGCCCGAGGCG
GTCGCGACCGGGGAGCCGCGGCTGCGGATCCGGTCCGGTCAGGTGTCCGTACCCCGGCTG
GCGCCCGTGCCGCACGCCGGCTCCAAGGCCGCCGCCTCCCCGGTCTTCTCGCCGGAGGGC
ACTGTCCTGGTGACCGGCGGCACCGGCAGGCTCGGCGCCCTGCTGGCCCGGCACCTCGTC
GCGCGTCACGGCGTACGGCACCTGCTGCTGGTCAGCCGCCGGGGAGCGGACGCACCCGGA
GCCGCCGAGCTGACGGCGGAACTGCGCGACGCCGGCGCCCGGGTCACCGTGAGGGCCTGT
GACGCCGCCGACCGGGAGGCACTGGCCGCCGTGCTGGAGGACGTACCGGCTCCACACCCG
CTCACCGCGGTGATCCACACGGCCGGTGTACTGGACGACTGCGTCGTGGAGCGGCTGACA
CCGGGACGGCTGGACGCGGTGCTGCGGCCCAAGGCCGACGCCGCGTGGCACCTGCACGAG
CTGACCCGGGACCTGGGCCTGCGGGCCTTCGTCCTGTTCTCGTCCGTCTCCGGGCTGATC
GGCACGGCCGGACAGGCCAATTACGCTGCGGCCAACGCGTTCCTCGACGCGCTGGCCGGG
CACCGTCGACGGCTCGCCCTCCCGGCGACCTCCCTCGCCTGGGGGCTGTGGGCGGGCGAC
GGCGGCATGGCCGGGGCGCTCGGCGCCCAGGACATCGCCCGGTTCGCCCGCGAGGGCATC
GCGCCGCTGGAGCCGGAGGAGGCGCTGCGTCTGTTCGACGCGGCCCTGGCGCACGAGGAG
GCGCTTCTCGCACCGGTGCGCGTGGACCGGGCGGCGCTGCGTCGGCGCGCGCGGTCCGGT
GCGGACATCTCGCCACTGATGCGGGAGGCGGCCGGCGGAACGCCGCGCGCCTGCGAGCAC
GACGGGAGCGGGGCGGTCCCACTGTCCGGGCGGCTCGCCGGCCGGTCCGAGGCGGAGCGC
CGACGCGTCCTGTCGGACGTGGTCCACACCCAGGCCGCGGCCGTGCTCGGCTTCGCCGAC
CCGAGCGGCATCGTGTCCGAACGGGCCTTCAAGGAGCTGGGCTTCGACTCGCTGACCTCG
GTGGAACTGCGCAACCGGCTCAACTCGGCGACCGGATTGCGGCTGTCGGCGGGCGTGATC
TTCGAGCATCCGACACCGCGGGCGCTGGCCGACCACCTCGCGGAACTGCTGCACCCCGCA
GACCGGGACCGGCCCGCCGCCCCCGCACTCCTCACCTCACCCCCGGCGCCCTCCGACACC
ACCGACCCCGACAACGACGCCATCGCCATCATCGGCATCGGCTGCCGCTTCCCCGGCGGA
ATCACCACCCCCGAACAACTCTGGCAACTCCTCACCGAAGAACGCCACGCCATCACCGAC
TTCCCCACCAACCGCGGCTGGCCCGACAACCTCCACCACCCCGACCCCACCCACCCCGGC
ACCACCTACACCCGCAAAGGCGGCTTCCTCCACGACGCCGACCAGTTCGACGCCCACTTC
TTCGGCATCTCCCCCCGCGAAGCCCTCGCCATGGACCCCCAGCAACGCAGCCTCCTCGAA
ACCGCCTGGCACACCCTCGAACACGCCGGCATCGACCCCACCACCCTCCACGGCACCGAC
ACCGGCGTCTTCGTGGGCATGATGTACCACGACTACGCGCCGGCCGCGGGGCAGATGCCC
AGGGACCTGGAGGGGCTGCTGCTGACCGGGAACCTCGGCAGTGTGCTCTCGGGGCGGCTG
GCGTACCAATTCGGGTTCACCGGCCCCGCGCTCACCCTCGACACGGCCTGCTCCTCCTCC
CTCGTCGCCCTCCACACCGCCACCCAAGCCCTCCGCAACAACGAATGCACCCTCGCCCTC
GCCGGCGGCACCACCATCATGGCCACCCCAGGCACCTTCATCGAATTCAGCCGCCTCCAA
GGACTCTCCCCCGACGGACACTGCCGCTCCTTCTCCGCCCACGCCAACGGCACCGGCTGG
TCCGAAGGAACAGCCCTCCTCCTCCTCGAACCCCTCACCCAAGCACAACGCAACAACCAC
ACCGTCCACGCCGTCATCCGCGCCACCGCCACCAACCAGGACGGCCCCTCCAACGGCCTC
ACCGCCCCCAACGGCCAAGCCCAACAACGCCTCATCCACCACACCCTCACCACCGCCCAC
CTCACCCCCCACGACATCGACGCCATCGAAGCCCACGGCACCGCCACCCCCCTCGGCGAC
CCCATCGAAGTCCAGGCACTCCACACCGTCTACGGCCAAGACCGCACCCGCCCCCTGTGG
CTCGGCTCCCTCAAGTCCAACCTCGGGCACACCCAAGCCGCCGCCGGAGCCGCCGGCGTC
ATCAAAATGATCCACTAG

[1] KS	48..422

[1] AT	574..886

[1] malonyl-CoA	759..763

[1] DH	931..1091

[1] KR	1380..1560

[1] ACP	1664..1734

[2] KS	1766..2124

[1] KS	142..1266

[1] AT	1720..2658

[1] malonyl-CoA	2275..2289

[1] DH	2791..3273

[1] KR	4138..4680

[1] ACP	4990..5202

[2] KS	5296..6372

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like G3DSA:1.10.1200.10 ACP_like PF00550 PP-binding PS50075 ACP_DOMAIN IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020807 Polyketide synthase, dehydratase domain (Domain) SM00826 PKS_DH IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	No significant hit
TMHMM	No significant hit

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