Heda_00040 Heda_00040   Heda_00060 Heda_00060

Heda_00050 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction14,167 / 11,885 / - [in whole cluster]
14,167 / 11,885 / - [in contig]
Locationcomplement(11885..14167) [in whole cluster]
complement(11885..14167) [in contig]
TypeCDS
Length2,283 bp (760 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productputative UvrA-like protein
Product (GenBank)UvrA-like drug resistance protein
GeneorfH11
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf28
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

orfH11: UvrA-like drug resistance protein

配列解析のみ。
DNA除去修復に関連したE.coli and Micrococcus luteusのUvrAに強い配列類似あり。
vitroでDNR抵抗性に重要だと確認されているUvrA-like protein DrrCにも66%相同性あり。
Related Reference
ACC
Q54827
NITE
Adria_00370
PmId
[8655504] The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production. (J Bacteriol. , 1996)
[9835035] The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin. (FEMS Microbiol Lett. , 1998)
comment
Blast 31st, id53%, 0.0
Streptomyces peucetius_drrC
Daunorubicin resistance protein

--
[PMID: 8655504](1996)
DrrCはDNAの除去修復に関連するE.coli_UvrAに強いAA配列類似あり。
S.lividansへのdrrC導入はDNR抵抗性を与える。
DNR and DXR antiporterによって抵抗性をもたらすDrrABのシステムと異なり、たぶんゲノムDNAへのDNR and DXRの結合を抑制または不安定化をしうる。

--
同じ著者らの続報
[PMID: 9835035](1998)
E.coliで発現して得たDrrCは、ATPとDNRの存在下でのみ、DNR産生遺伝子のpromoter領域を含むDNA segmentを結合。
S.peucetiusにおけるDrrCの出現はDNR産生の開始と同時に起こり、drrC geneはDNRによって誘導される。
DnrN and DnrI regulatory proteinsはdrrC発現のために必要。
DrrAは、DrrCと同じ様式で調節された。

--
参照[PMID: 20158521](2010)
この論文での実験にdrrCは絡んでいないが、総合的なFigあり。

細胞内DNR濃度が上昇したときに、DNR biosynthetic genes and resistance genesのactivatorであるDnrI のpromoter領域へfeedback阻害のために結合したDNR（本来はDnrNが結合）を、再活性化のために離すことに関連しているのではないかと言っている。

close this sectionSequence

selected fasta
>putative UvrA-like protein [UvrA-like drug resistance protein]
MTTTPDTGSDDGVRIVGARENNLKDVSLTIPRNRITVFTGVSGSGKSSLVFDTLAVESQR
QLYATFNWFIRNQLPRYGRPHVTAVENLTAPVVVDQKPLGGNARSTVGTLTDLSSTLRVL
FSHCAEPALPPGAYSFNDPQGMCPQCEGLGQERQVDVERMLDRSKSLDEGAILLPTHKVG
SMDWQQYANSGHFDTSKRLCDYTEQEWHTLLHGSGGTVTVRTANTTTTLAYEGVVARFAR
TNLKRDLSTLGERARANVERFVSLGPCTSCGGARLAPPTLASTIEGRTIADLSRMEVGEL
VPFLETITDPVGAQIAADLREGLSRVVAIGLGYLSLDRSSTSLSGGEAQRLKMVRHLSSS
LTGMTYVFDEPSTGLHPQDVGRLGELMRALCDRGNTLVVVEHDPDVIALADHVVDLGPGA
GPHGGEVVYTGPFGGLLGADTVTGRALRRRGTVKPEARRPTGKLSVEGADLNNLRNVSVA
FPTGVLTAVTGVAGSGKSSLVTGAFRQAHPDAVFVDQSPVRASARSTPVSFLQAMDPLRK
LFARATGAPAGLFSFNSAGACGTCQGRGEIVTELAYMDPVRLPCEGCGGRRFQEEVLRHR
LRGRSVADVLEMTAQDALDFFTEPEITGRISRLVDVGLPYLTLGRSLSTLSGGERQRLKL
AGQLERSHGVYVLDEPTTGLHRADVDGLVALLDRLVDRGNTVIVVEHDLEVVRRADWVVD
LGPGGGRHGGEVVFTGTPAALLAAEGSVTGEHLRRFCAGG
selected fasta
>putative UvrA-like protein [UvrA-like drug resistance protein]
GTGACGACAACGCCGGACACGGGTTCCGACGACGGCGTCAGGATCGTCGGAGCCCGTGAG
AACAACCTCAAGGACGTGTCGCTGACGATCCCGAGGAACCGGATCACCGTGTTCACCGGG
GTGTCCGGCTCGGGCAAGTCGTCGCTGGTGTTCGACACCCTGGCGGTGGAGTCCCAACGG
CAGTTGTACGCCACCTTCAACTGGTTCATCCGCAACCAGCTTCCCCGCTACGGCCGCCCG
CACGTGACCGCGGTGGAGAACCTGACCGCTCCCGTCGTGGTCGACCAGAAGCCGCTCGGC
GGCAACGCGCGCTCCACCGTCGGCACCCTGACGGACCTGTCGTCGACGCTGCGCGTGCTG
TTCTCCCACTGCGCCGAGCCGGCCCTGCCGCCCGGTGCGTACTCCTTCAACGACCCGCAG
GGCATGTGCCCGCAGTGCGAGGGCCTGGGGCAGGAGCGCCAGGTGGACGTGGAGCGGATG
CTGGACCGCTCCAAGTCCCTGGACGAGGGTGCCATCCTGCTGCCCACCCACAAGGTGGGC
AGCATGGACTGGCAGCAGTACGCCAATTCCGGCCACTTCGACACCTCCAAGCGGCTGTGC
GACTACACCGAGCAGGAGTGGCACACCCTGCTGCACGGCAGCGGCGGCACGGTGACCGTG
CGCACGGCGAACACCACGACCACCCTCGCCTACGAGGGCGTCGTCGCCCGTTTCGCCCGT
ACCAACCTCAAGCGCGACCTGAGCACCCTCGGCGAGCGCGCCCGGGCCAACGTGGAGCGG
TTCGTCTCCCTCGGCCCGTGCACGTCCTGCGGCGGCGCCCGGCTGGCTCCGCCGACCCTG
GCGTCGACGATCGAGGGGCGCACCATCGCCGACCTCTCCCGGATGGAGGTCGGCGAACTC
GTCCCGTTCCTGGAGACGATCACGGACCCGGTGGGGGCGCAGATCGCGGCCGACCTGCGC
GAAGGGCTGAGCCGGGTGGTGGCGATCGGTCTGGGCTACCTGTCCCTGGACCGCTCCTCC
ACCTCACTGTCCGGCGGCGAGGCGCAGCGGCTGAAGATGGTGCGGCACCTCAGCAGCAGC
CTGACGGGCATGACGTACGTCTTCGACGAACCCAGCACCGGTCTGCACCCGCAGGACGTG
GGCAGGCTCGGCGAGTTGATGCGAGCCCTGTGCGACCGGGGCAACACCCTGGTCGTCGTC
GAGCACGACCCGGACGTCATCGCCCTGGCCGACCATGTCGTCGACCTCGGTCCGGGGGCC
GGCCCGCACGGCGGCGAGGTCGTGTACACGGGACCCTTCGGGGGGCTGCTCGGGGCGGAC
ACCGTCACCGGCCGGGCCCTGCGGCGCCGGGGCACGGTCAAGCCGGAGGCCCGGCGGCCC
ACCGGGAAGCTGTCCGTGGAGGGAGCGGACCTGAACAACCTCCGGAACGTGTCCGTCGCC
TTCCCGACCGGGGTGCTGACGGCGGTGACCGGGGTCGCGGGGTCCGGCAAGAGCTCCCTG
GTCACCGGGGCGTTCCGCCAGGCGCACCCGGACGCGGTCTTCGTCGACCAGTCCCCGGTG
CGGGCCTCCGCGCGGTCCACGCCGGTGAGCTTCCTCCAGGCGATGGATCCGCTGCGCAAG
CTGTTCGCCAGGGCCACCGGCGCTCCGGCCGGTCTGTTCAGCTTCAACTCCGCCGGGGCC
TGCGGCACGTGCCAGGGCCGGGGCGAGATCGTCACGGAGCTGGCCTATATGGACCCGGTG
CGGCTGCCCTGCGAGGGGTGCGGGGGCCGCCGGTTCCAGGAGGAGGTGCTCCGGCACCGG
CTGCGCGGCCGGTCCGTCGCGGACGTGCTGGAGATGACCGCGCAGGACGCCCTGGACTTC
TTCACCGAGCCGGAGATCACCGGCCGGATCAGCCGTCTCGTCGACGTCGGGCTGCCGTAC
CTCACCCTAGGCAGGTCGCTGAGCACCCTGTCCGGGGGCGAGCGGCAACGGCTGAAACTG
GCGGGCCAGCTGGAGCGCTCGCACGGCGTGTACGTGCTCGACGAACCGACCACCGGCCTG
CACCGGGCGGACGTGGACGGCCTGGTCGCGCTGCTGGACCGCCTGGTCGACCGGGGCAAC
ACCGTGATCGTCGTGGAACACGACCTGGAGGTGGTGCGGCGCGCGGACTGGGTGGTCGAC
CTCGGTCCCGGCGGCGGCCGGCACGGCGGCGAGGTGGTGTTCACCGGCACACCGGCGGCG
CTGCTGGCCGCCGAGGGGTCGGTCACGGGCGAGCATCTGCGGCGGTTCTGCGCGGGCGGC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[447-748] PS50893
PS50893   ABC_TRANSPORTER_2
[611-678] 4.5e-09 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[32-420] 0.01 SM00382 [483-725] 1.10000029896148e-06 SM00382
SM00382   AAA
IPR017871 ABC transporter, conserved site (Conserved_site)
[650-664] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit
Heda_00040 Heda_00040   Heda_00060 Heda_00060
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