Heda_00070 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	15,991 / 14,549 / - [in whole cluster] 15,991 / 14,549 / - [in contig]
Location	complement(14549..15991) [in whole cluster] complement(14549..15991) [in contig]
Type	CDS
Length	1,443 bp (480 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	2,3-hexose dehydratase
Gene	hedP
Gene (GenBank)
EC number
Keyword	alpha-L-N,N-dimethylvancosamine beta-D-angolosamine
Note
Note (GenBank)	orf26
Reference	ACC Q67G51 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 hedP: 2,3-Hexose dehydratase hedPについては配列解析のみ。 hedamycinにある2つのsugar moietyの共通生合成経路に割り当てられている。 NDP-4-keto-6-deoxy-D-glu -(HedP)-(HedM)→ NDP-3-amino-4-keto-2,3,6-deoxy-D-glu
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 35th, id51%, 1e-119 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [2,3-hexose dehydratase]
MTTHRRKAGTRTPAETAASLAKSAAVRSGPSVGGREARRWLAEQRRRTVMDVRQIPLDRL
TGWHTDPATGDLVHASGRFFAIAGLDVQRPGTPVPSWQQPVIEQPEVGVLGILVREFDGV
LHCLMQAKAEPGNCNRVQLSPTVQATRSNYTRVHEGASVPYLEFFRRPSRHRVLADVRQS
EQGSWFHGKRNRNMVVQVDEDVAVLDGFRWFTLREIHELFAVDDAVNMDTRTVLSCLPYV
GPRLPEVLGTGRDPFTSALLRSCSGLEGGRHGMTEILSWITDERTRTVNEARRVPLKEVA
HWQRRDGRITHASGLFFNVIGVSVTADGREVAAWSQPLLEPCDEGVAAFLAKPVDGVLHV
LVHARAEPGFTDVVELGPTVQCTPSNYAVLPPAARPPFLDEVLHAEAGRVRFDAVHSEEG
GRLYHARNRYVVVETPEDFEPDHCGYRWLTLAQLSRLLRHSNYVNVQARSLVACLYSMTA

>putative NDP-hexose 2,3-dehydratase [2,3-hexose dehydratase]
GTGACGACCCATCGCCGGAAAGCCGGAACCCGGACGCCGGCCGAGACGGCCGCGTCGCTC
GCCAAGTCGGCGGCCGTGCGGTCCGGACCATCGGTCGGCGGCCGGGAAGCCAGGCGCTGG
CTGGCCGAGCAGCGCCGGCGCACGGTGATGGACGTCCGGCAGATACCGCTGGACAGGCTG
ACGGGCTGGCACACCGATCCGGCAACCGGTGACCTCGTGCACGCGTCGGGGAGGTTCTTC
GCGATCGCCGGGCTCGACGTGCAGCGGCCCGGCACTCCCGTGCCGTCCTGGCAGCAGCCG
GTCATCGAGCAGCCCGAGGTGGGTGTGCTGGGCATCCTGGTGCGGGAGTTCGACGGTGTC
CTGCACTGCCTGATGCAGGCCAAGGCGGAGCCCGGCAACTGCAACCGCGTGCAGCTGTCG
CCGACGGTCCAGGCCACCCGCAGCAACTACACGCGGGTGCACGAGGGGGCTTCGGTGCCG
TACCTGGAGTTCTTCCGGCGGCCGTCCCGGCACCGGGTGCTCGCCGACGTGCGGCAGTCG
GAGCAGGGCTCGTGGTTCCACGGCAAGCGCAACCGCAACATGGTGGTACAGGTGGACGAG
GACGTGGCGGTCCTCGACGGTTTCCGCTGGTTCACCCTTCGCGAGATCCACGAGCTGTTC
GCCGTCGACGACGCCGTCAACATGGACACCCGCACGGTCCTGTCCTGCCTGCCCTACGTC
GGTCCGCGGCTGCCGGAGGTCCTGGGAACGGGCCGGGACCCGTTCACCTCCGCTCTGTTG
CGGTCCTGCTCCGGCCTGGAGGGGGGCCGGCACGGCATGACCGAGATCCTGAGCTGGATC
ACGGACGAGCGGACCCGCACGGTCAACGAGGCGCGGCGCGTCCCGCTGAAGGAGGTGGCG
CACTGGCAGCGCCGGGACGGCCGCATCACCCATGCCAGCGGGCTGTTCTTCAACGTGATC
GGGGTCAGCGTGACCGCCGACGGCCGGGAGGTCGCCGCCTGGTCGCAACCGCTGCTGGAG
CCCTGCGACGAGGGGGTCGCGGCCTTCCTCGCCAAGCCGGTGGACGGGGTGCTGCACGTG
CTGGTGCACGCTCGCGCCGAACCCGGTTTCACGGACGTCGTGGAGCTGGGTCCGACCGTG
CAGTGCACTCCGTCGAACTACGCCGTGCTGCCCCCGGCCGCCCGTCCGCCGTTCCTGGAC
GAGGTGCTGCACGCGGAGGCCGGGCGGGTCAGGTTCGACGCGGTGCACTCCGAGGAGGGC
GGCCGCTTGTACCACGCCCGCAACCGCTACGTGGTCGTGGAGACCCCCGAGGACTTCGAA
CCCGACCACTGCGGATACCGCTGGCTGACCCTGGCCCAGCTCTCCCGGCTGCTGCGCCAC
AGCAACTACGTCAACGTGCAGGCCCGCAGCCTGGTGGCCTGCCTGTACAGCATGACCGCT
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Heda_00060 Heda_00080