Heda_00120 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	19,783 / 20,748 / + [in whole cluster] 19,783 / 20,748 / + [in contig]
Location	19783..20748 [in whole cluster] 19783..20748 [in contig]
Type	CDS
Length	966 bp (321 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	diphospho-4-keto-2,3,6-trideoxyhexulose reductase
Gene	hedN
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf21
Reference	ACC Q67G46 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 hedN: Diphospho-4-keto-2,3,6-trideoxyhexulose reductase 配列解析のみ。 Figure 2だとORFの向きが逆。 beta-D-angolosamine (3-dimethylamino-2,3,6-trideoxy-beta-D-arabino-hexopyranose)の生合成での4-KRに割り当てられている。参照しているのはvancomycin gene clusterでのdTDP-L-vancosamine生合成経路。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Blast 7th, id44%, 4e-50 Streptomyces peucetius_dnmV Thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [Adria_00200]NDP-hexose C-4 ketoreductase -- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 -- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [diphospho-4-keto-2,3,6-trideoxyhexulose reductase]
MPAQPSALVLGGTGFVGRHVCEGLDRSGFDVVAVARRPPADSVGDRFRPLDLGRQPVGEV
AAVLAETSPAVVVNAVGSIWGHSDPEMWDAAAAPTLRLLDALALSGARPRVIHLGSVLEY
GRVEAGTTMGAATAARPTSAYGRAKLAATRAVLDRVRAGRFDGMVLRAANLAGPGSPDVS
LLGRVATRLAQAGPDGVARVELDPLLARRDYVDVRDVADAVVAAATTSLSGELVDIGRGE
SVPVRTLVDLLIARSGVPARVVERPGAGIRHSTEEWSRVDIAPAARLLGWRPRRSLAEAV
EDFWHDFLRRHHTTDQKRSAH

>putative NDP-hexose C-4 ketoreductase [diphospho-4-keto-2,3,6-trideoxyhexulose reductase]
ATGCCTGCTCAGCCCAGCGCTCTGGTACTCGGCGGCACAGGCTTCGTGGGACGGCACGTG
TGCGAGGGACTCGACAGGAGCGGTTTCGACGTCGTCGCCGTGGCCCGCAGACCGCCCGCC
GATTCCGTGGGCGACCGGTTCCGTCCCCTCGACCTCGGCCGGCAGCCGGTCGGGGAGGTG
GCCGCCGTGCTGGCCGAGACCTCCCCCGCGGTGGTCGTCAACGCGGTCGGCAGCATCTGG
GGACACAGCGACCCGGAGATGTGGGACGCGGCCGCGGCGCCGACCCTGCGGCTCCTCGAC
GCACTCGCGCTGTCCGGCGCACGGCCCCGTGTGATCCACCTGGGATCGGTCCTGGAGTAC
GGGCGGGTCGAGGCCGGCACCACCATGGGCGCCGCCACTGCCGCCCGTCCGACCAGTGCG
TACGGGCGCGCCAAGCTGGCGGCGACCCGAGCCGTCCTGGACCGGGTGCGCGCCGGCCGG
TTCGACGGCATGGTGCTGCGGGCAGCGAACCTGGCCGGTCCGGGCAGCCCGGACGTGAGC
CTGCTCGGCCGGGTGGCCACCCGGCTGGCGCAGGCCGGCCCGGACGGCGTGGCCCGGGTG
GAGCTGGACCCGCTGCTGGCCCGCCGGGACTACGTCGACGTGCGGGACGTCGCCGACGCG
GTGGTGGCCGCCGCCACCACCTCGCTCTCCGGCGAGCTGGTCGACATCGGCAGAGGAGAG
AGCGTGCCCGTGCGCACGCTCGTGGACCTGCTCATCGCCCGCAGCGGGGTGCCCGCCCGT
GTCGTCGAACGGCCGGGCGCCGGCATCCGGCACTCCACCGAGGAGTGGTCCCGGGTGGAC
ATCGCTCCGGCCGCGCGGCTGCTCGGCTGGCGGCCGCGGCGCTCCCTCGCGGAGGCGGTG
GAGGACTTCTGGCACGACTTCCTACGGCGACACCACACGACCGACCAGAAACGGAGCGCA
CACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota
TMHMM	No significant hit

Heda_00110 Heda_00130