Heda_00150 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	23,954 / 23,172 / - [in whole cluster] 23,954 / 23,172 / - [in contig]
Location	complement(23172..23954) [in whole cluster] complement(23172..23954) [in contig]
Type	CDS
Length	783 bp (260 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter permease protein
Product (GenBank)	ABC transporter
Gene	orfH7
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf18
Reference	ACC Q67G43 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 orfH7: ABC transporter 配列解析から、OrfH6 and OrfH7はABC transporter systemsを形成すると提唱されている。 orfH6 and orfH7は、抗生物質産生のある放線菌のABC transporter systemsをコードする遺伝子に似ている。avilamycin生合成gene clusterのresistance factor AviABC1-AviABC2に高い相同性あり。
Related Reference	ACC Q9F5K3 NITE Avil_00360 PmId [11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001) comment Blast 85th, id38%, 4e-35 Streptomyces viridochromogenes_AviABC2 Putative transmembrane protein S. lividans TK66にてaviABC1, aviABC2, aviRa, aviRb発現。 --> avilamycin resistance (avilamycin A inhibited poly(U)-directed polyphenylalanine synthesis)
Related Reference	ACC P32011 NITE Adria_00140 PmId [1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991) [9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997) [15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005) [19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 169th, id34%, 1e-27 Streptomyces peucetius_drrB Daunorubicin/doxorubicin resistance ABC transporter permease protein drrB -- [PMID: 1924314](1991) DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。 doxorubicin(DXR) or daunorubicin(DNR)の添加で活性が上昇。DrrBはABC-2 type transporterといわれているもの。 -- さらにいくつかの続報がでており、DrrABはATP依存的なDXRとDNRの排出ポンプとされている(PMID: 15709779)。上記著者らでの最新の続報は[PMID: 19063901](2009) -- 違うグループでも報告あり。上記既報をrefとして採用している。 [PMID: 20158521](2010) drrAB(-)mutantはDNRに高い感受性あり。このmutantはwildと比べて、薬剤産生が10倍減。 key regulator dnrI and polyketide synthase gene dpsAの発現が激減。 regulatory genes dnrO and dnrNの発現は増。これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

Sequence

>putative drug resistance ABC transporter permease protein [ABC transporter]
MSDALTLSRRSLVKNLRNPDVVVFSSAAPIAFALLFGYVFGSAIDLGGPNYREFMVVGIL
TQTMLLTASNTGTGIAYDMKQGLVDRFRSLPISQSAVLFGRTNGDAVNNLVVMALLSVTG
LLMGWRIRGSFADAALAYLLLFLFAYAASWIAATIGLSVRSPEVLGNVLLMALLPLTFVS
NAFVPSDELPGPMRVIAEWNPVSTVVAACRELFGNIPPGWPEPDAWPLQHPVAASFLWVA
VILLVFVPLAIRRYQRAVSQ

>putative drug resistance ABC transporter permease protein [ABC transporter]
GTGAGCGACGCCCTCACCCTGAGCCGGCGCAGCCTGGTCAAGAACCTGCGCAACCCTGAC
GTCGTCGTGTTCTCCAGCGCGGCCCCCATCGCCTTCGCCCTGCTCTTCGGCTATGTCTTC
GGCAGCGCGATCGACCTGGGCGGCCCGAACTACCGCGAGTTCATGGTGGTCGGCATCCTC
ACCCAGACGATGCTGCTCACCGCCTCCAACACCGGCACGGGCATCGCCTACGACATGAAG
CAGGGCCTGGTGGACCGCTTCCGGTCGCTGCCCATCTCCCAGTCCGCGGTGCTCTTCGGC
CGGACCAACGGCGACGCCGTGAACAACCTCGTGGTGATGGCGCTGCTGTCGGTGACCGGC
CTGCTGATGGGCTGGCGGATCCGCGGCTCGTTCGCGGACGCGGCCCTGGCCTATCTGCTC
CTGTTCCTGTTCGCGTACGCCGCGTCCTGGATCGCGGCGACGATCGGCCTGAGCGTCCGC
TCCCCCGAAGTGCTCGGCAACGTGCTGCTGATGGCACTGCTGCCGCTGACCTTCGTGTCC
AACGCCTTCGTGCCCAGCGACGAACTGCCCGGCCCGATGCGCGTGATCGCGGAGTGGAAC
CCGGTCTCCACGGTCGTCGCGGCCTGCCGCGAACTCTTCGGCAACATCCCGCCCGGCTGG
CCCGAGCCGGACGCGTGGCCGCTGCAGCACCCGGTTGCGGCGTCCTTCCTGTGGGTGGCG
GTCATCCTGCTGGTCTTCGTACCGCTGGCCATCCGCCGCTACCAACGGGCCGTCAGTCAG
TAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000412 ABC-2 (Family) PS51012 ABC_TM2 IPR013525 ABC-2 type transporter (Domain) PF01061 ABC2_membrane
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	Transmembrane 6

Heda_00140 Heda_00160