Heda_00290 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	40,439 / 41,404 / + [in whole cluster] 40,439 / 41,404 / + [in contig]
Location	40439..41404 [in whole cluster] 40439..41404 [in contig]
Type	CDS
Length	966 bp (321 aa)

Annotation

Category	3.4 other modification
Product	putative cyclase/dehydratase
Product (GenBank)	cyclase
Gene	hedB
Gene (GenBank)
EC number
Keyword	2nd ring
Note
Note (GenBank)	orf4
Reference	ACC Q67G29 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 hedB: Cyclase 配列解析のみ。 Figure 2だとORFの向きが逆。
Related Reference	ACC Q02057 NITE Actino_00220 PmId [11851466] Genetic Contributions to Understanding Polyketide Synthases. (Chem Rev. , 1997) comment Blast 4th, id54%, 4e-44 Streptomyces coelicolor_SCO5091 Cyclase [Actino_00220]cyclase/dehydratase_actIV(actIORF5) -- [Pubmed登録なし]Engineered Biosynthesis of Novel Polyketides: actVII and actIV Genes Encode Aromatase and Cyclase Enzymes, Respectively. J. Am. Chem. Soc., 1994, 116 (24), pp 10855-10859 act minimal PKS, KR(actIII ), actVII, actIV の様々な組み合わせのrecombinantsによって産生されたpolyketides構造を測定。 minimal PKS = SEK4 minimal PKS + act KR = mutactin minimal PKS + act KR + actVII = SEK34 minimal PKS + act KR + actVII + actIV = DMAC ActVII は1st carbocyclic ringの2つのdehydrationsを触媒することを担うaromataseとして機能する。 AcyIV は2つめのringを形成するための分子内aldol縮合を触媒するcyclase(aldolase)である。 ActVII homologsは、gra, fren, tcm, gris生合成clusterにある。 ActIV homologsは、gra, fren, gris生合成clusterにある。 -- [PMID:11851466](1997) 上記PMIDなし論文がRef採用されているレビュー。
Related Reference	ACC Q9X466 NITE Naphcy_00060 PmId [10206788] Isolation and characterization of the naphthocyclinone gene cluster from Streptomyces arenae DSM 40737 and heterologous expression of the polyketide synthase genes. (Gene. , 1999) comment Blast 9th, id57%, 4e-42 Streptomyces arenae__ncnE Cyclase [Naphcy_00060]cyclase/dehydratase NcnE: cyclase actIVとid55.2% ncnE + actKR, PKS, ARO → 3,8 DMAC and aloesaponarin II産生 act KR, PKS, ARO, CYCでも同じもの産生。よってNcnE = act CYC(actIV gene) ActIVはC5-C14 2nd ring cyclizationを担う。

Sequence

>putative cyclase/dehydratase [cyclase]
MDGTFRPLPDDERASEIEVTFTPEPGGGTRVELAHTRLDKLGDSDGERIFKALDGPSPGE
TLARFAEAGVMAVAHTVAGPGLLEIADGVYAREQLPGGWCLNNAGLVAGGGRAVLVDTAA
TRARARHLAAAVDAVAPGGPDVVVNTHFHGDHVFGNVEFAPRATVVAHEQTRSDVAESGH
GLCRLWPTVDWGDIPLVLPDVTFEDELTFHAGDLTVHLLHVGPAHTPGDVVAWVPERKVL
FARGRRVVPGHAVRPDGLGARLSGGPGAPARSPARGGGRRARTGVRHRGTGRHRGVPPPP
RRRRPGGAARRPHPARRRTGG

>putative cyclase/dehydratase [cyclase]
GTGGACGGCACCTTCCGGCCGCTGCCCGACGACGAACGGGCCAGCGAGATCGAGGTGACC
TTCACCCCGGAACCGGGCGGCGGCACCCGGGTCGAGCTGGCGCACACCCGGCTCGACAAG
CTCGGCGACAGCGACGGCGAACGCATCTTCAAGGCCCTGGACGGGCCGAGCCCCGGTGAG
ACGCTGGCCCGGTTCGCCGAGGCCGGTGTGATGGCCGTTGCGCACACCGTCGCCGGACCC
GGCCTGCTGGAGATCGCCGACGGCGTCTACGCCCGTGAACAACTGCCCGGCGGCTGGTGT
CTGAACAACGCCGGTCTGGTGGCCGGCGGCGGTCGTGCGGTCCTCGTCGACACCGCGGCC
ACCCGGGCCCGGGCCCGTCATCTCGCCGCAGCGGTGGACGCGGTGGCGCCCGGCGGTCCG
GACGTCGTGGTCAACACCCACTTCCACGGCGACCACGTCTTCGGCAACGTCGAGTTCGCC
CCCCGGGCCACCGTCGTCGCCCACGAGCAGACCCGGTCCGACGTGGCGGAGTCCGGACAC
GGGCTCTGCCGGTTGTGGCCGACGGTGGACTGGGGCGACATCCCCCTGGTACTGCCCGAC
GTGACCTTCGAGGACGAACTCACCTTCCACGCCGGAGACCTGACGGTCCATCTGCTGCAC
GTCGGACCCGCGCACACCCCCGGCGACGTGGTGGCCTGGGTGCCGGAGCGCAAGGTGCTC
TTCGCCCGGGGACGTCGTGTGGTCCCGGGTCACGCCGTTCGTCCTGATGGGCTCGGTGCA
CGGCTCTCTGGAGGCCCTGGAGCGCCTGCGCGGTCTCCGGCCCGAGGTGGTGGTCGCCGG
GCACGGACCGGTGTCCGGCACCGAGGCACTGGACGCCACCGAGGCGTACCTCCGCCTCCT
CGGCGACGTCGCCCGGGAGGGGCTGCGCGCCGGCCGCACCCCGCTCGCCGCCGCACGGGC
GGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001279 Beta-lactamase-like (Domain) PF00753 Lactamase_B SM00849 Lactamase_B
SignalP	No significant hit
TMHMM	No significant hit

Heda_00280 Heda_00300