Heda_00320 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	44,086 / 45,603 / + [in whole cluster] 44,086 / 45,603 / + [in contig]
Location	44086..45603 [in whole cluster] 44086..45603 [in contig]
Type	CDS
Length	1,518 bp (505 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance transporter
Product (GenBank)	membrane transport protein
Gene	orfH1
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf1
Reference	ACC Q67G26 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 orfH1: Membrane transport protein 配列解析のみ。 mitomycin生合成gene clusterの新規drug export systemに関連したMctに有意な相同性あり。
Related Reference	ACC Q9WVV3 PmId [10198016] Mitomycin resistance in Streptomyces lavendulae includes a novel drug-binding-protein-dependent export system. (J Bacteriol. , 1999) comment Blast 9th, id44%, 8e-76 Streptomyces lavendulae_mct Mct(Putative mitomycin C translocase) mct insertion不活化mutantではmitomycin Cの感受性がかなり強くなる。 E.coliでmctを発現するとmitomycin C抵抗性が5倍に増え、細胞内の薬剤蓄積が減る。 mctとmrdを一緒にE.coliで発現すると、抵抗性は150倍になる。

Sequence

>putative drug resistance transporter [membrane transport protein]
MRHAPIIPRVSTAAARGATFLTCIETLGRSRAWCWRRPPSPNSSSPWTCPWSTSPCPRSV
LPWDSHHSTCRGSVHVYALTFGGFLLLGGRACDLYGRRRMFVLGLIVFGLCSLAGGLAQT
PWHLIAARAGQGLGAAAAAPAALAMLTTTFAEGPRRVRALGVWSAVNAAGGALGVLAGGL
LTEYAGWRWVMLVNLPIVAAALALIAAGVPATAASAPRERLDALGAALATGGVGLLVLGV
VRTDTLGWDSPLTWATLAAAAVLLAAFLFAESRADAPLLRLGLLRSRWVAGANVLVFLAA
AGQFTAFYFVSLYMQQVLGMGAAATGIAFLPFSLGLVAGTVVATRITAARTPRASLVPGA
LLAAAGLAWFALISPDGGFLTDVLGPSLVTSVGAGLVLAPVAAAATTGVAAREAGMASGL
MNSSRQLGGSIGLAVLATLAAHHTGTATDPAALNDGYGLGLAVAAAVFVLAAVVAIGVLP
RRRRPVAPVPRSTASEENELEGTPS

>putative drug resistance transporter [membrane transport protein]
TTGCGGCATGCCCCCATCATCCCTCGGGTGTCGACAGCGGCGGCCCGCGGCGCTACTTTT
CTTACATGTATAGAAACTCTCGGGAGAAGTCGAGCGTGGTGCTGGCGGCGGCCGCCGTCG
CCCAATTCGTCGTCGCCCTGGACCTGTCCGTGGTCAACGTCGCCCTGCCCGCGATCCGTA
CTGCCCTGGGATTCGCACCACTCGACCTGTCGTGGGTCCGTGCACGTGTACGCCCTTACG
TTCGGCGGGTTCCTGCTGCTGGGAGGCCGCGCCTGCGACCTGTACGGCCGGCGTCGGATG
TTCGTGCTGGGACTGATCGTCTTCGGCCTGTGCTCGCTGGCCGGCGGGCTGGCCCAGACG
CCCTGGCATCTGATCGCCGCACGCGCCGGGCAGGGCCTGGGCGCCGCCGCGGCCGCACCG
GCCGCACTGGCGATGCTCACCACCACCTTCGCCGAAGGGCCCCGGCGCGTCCGCGCGCTC
GGAGTGTGGAGCGCGGTGAACGCCGCGGGCGGCGCGCTGGGCGTGCTGGCGGGCGGGCTG
CTGACCGAGTACGCGGGCTGGCGCTGGGTTATGCTGGTCAACCTGCCCATCGTCGCCGCG
GCCCTCGCCCTCATTGCCGCAGGGGTGCCCGCCACAGCAGCGTCCGCCCCGCGCGAAAGG
CTGGACGCGCTGGGCGCCGCTCTGGCGACCGGCGGAGTCGGGCTGCTGGTGCTCGGTGTC
GTCCGCACCGACACCCTTGGCTGGGACTCCCCGCTCACGTGGGCGACCCTCGCTGCCGCT
GCCGTACTGCTGGCCGCCTTCCTCTTCGCCGAGTCGAGGGCCGACGCACCGCTGCTGCGC
CTCGGCCTGCTGCGCAGCCGCTGGGTCGCCGGCGCGAACGTGCTGGTGTTCCTCGCCGCG
GCCGGGCAGTTCACGGCGTTCTACTTCGTCTCCCTGTACATGCAGCAGGTCCTGGGCATG
GGAGCCGCAGCGACCGGCATCGCGTTCCTGCCCTTCTCCCTGGGCCTGGTCGCGGGGACC
GTCGTCGCGACCCGCATCACCGCGGCACGCACCCCCCGCGCCTCCCTGGTGCCCGGCGCC
CTGCTGGCCGCCGCGGGCCTGGCCTGGTTCGCCCTCATCAGTCCCGACGGCGGTTTCCTC
ACCGACGTGCTCGGCCCCTCCCTCGTCACCAGCGTCGGCGCCGGACTGGTCCTGGCCCCG
GTCGCCGCCGCCGCGACCACCGGAGTCGCCGCGCGGGAGGCCGGAATGGCCTCCGGCCTC
ATGAACAGCTCACGCCAACTCGGCGGCTCCATCGGGCTGGCCGTCCTGGCCACCCTTGCC
GCGCACCACACCGGCACAGCAACCGACCCCGCCGCGCTCAACGACGGCTACGGCCTGGGG
CTGGCCGTCGCCGCCGCCGTCTTCGTGCTCGCGGCGGTCGTGGCGATCGGTGTGCTGCCG
CGCCGCCGCCGGCCCGTCGCACCCGTCCCGCGATCCACCGCTTCTGAAGAGAACGAGCTG
GAAGGAACACCGTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011701 Major facilitator superfamily (Family) PF07690 MFS_1 IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS general substrate transporter IPR020846 Major facilitator superfamily domain (Domain) PS50850 MFS
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	Transmembrane 12

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