Jado_00050 Jado_00050   Jado_00070 Jado_00070

Jado_00060 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction6,401 / 8,155 / + [in whole cluster]
153 / 1,907 / + [in contig]
Location6401..8155 [in whole cluster]
153..1907 [in contig]
TypeCDS
Length1,755 bp (584 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative acyl-CoA carboxylase alpha subunit
Product (GenBank)JadJ
Gene
Gene (GenBank)jadJ
EC number6.4.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[10784049] An acyl-coenzyme A carboxylase encoding gene associated with jadomycin biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 2000)
comment
jadR1 and jadI の間にある584aaのORF = jadJ。

acyl-CoA carboxylasesの各componentと似ており、
N末はbiotin carboxylase (BC) component
C末はbiotin carboxyl carrier protein (BCCP) component
とalignmentが取れる。

jadJ破壊はjadomycin産生を激しく減らす。
成長や形態には影響しないのでFA合成に関与しない。

jadJは、抗生物質jadomycin Bを形成するためのpolyketide中間体生合成でmalonyl-CoAを供給する、と結論付け。
Related Reference
ACC
Q9X4K6
PmId
[10589718] Genetic and biochemical characterization of the alpha and beta components of a propionyl-CoA carboxylase complex of Streptomyces coelicolor A3(2). (Microbiology. , 1999)
comment
Blast 11th, id87%, 0.0
Streptomyces coelicolor_accA2
Putative acyl-CoA carboxylase complex A subunit

accA1, accA2のcloning, 配列決定している。

accA1のmutantはacetyl- or propionyl-CoA carboxylate活性にほとんど変化がみられなかったことから、complexesの形成に関与していないことが示唆された。しかしながら、accA1と非常に相同性が高いaccA2の欠損株を作れなったことから、in vivoでAccA1の代わりにAccA2が機能していた可能性も考えられた。

accA1, accA2, pccBをE.coliで発現させた結果、propionyl-CoA carboxylase beta-subunitがPccBであり、alpha-subunitがaccA1またはaccA2であることを確認(accA1+pccB, accA2+pccBどちらでも活性がみられた)。

accA2 mutantが作製できなかった点については、もしかすると他のcarboxyl transferasesにも関与しているかもしれないと考察している。
ACC
Q9RGQ6
PmId
[10589718] Genetic and biochemical characterization of the alpha and beta components of a propionyl-CoA carboxylase complex of Streptomyces coelicolor A3(2). (Microbiology. , 1999)
comment
Blast 12th, id87%, 0.0
Streptomyces coelicolor_accA1
Putative acyl-CoA carboxylase complex A subunit

close this sectionSequence

selected fasta
>putative acyl-CoA carboxylase alpha subunit [JadJ]
MRKVLIANRGEIAVRVARACRDAGIGSVAVYAEPDRDALHVRAADEAFALGGDTPATSYL
DMAKVLQAAADSGADAVHPGYGFLSENAEFAQAVLDAGLTWIGPPPQAIRDLGDKVAARH
IAQRAGAPLVAGTPDPVSGADEVVAFAEEHGLPIAIKAAFGGGGRGLKVARTLEEVPELY
DSAVREAVAAFGRGECFVERYLDKPRHVETQCLADQHGNVVVVSTRDCSLQRRHQKLVEE
APAPFLSEAQNAELYAASKAILKEAGYVGAGTVEFLVGTDGTISFLEVNTRLQVEHPVTE
EVTGIDLVREMFRIADGEELGYGDPEIRGHSFEFRINGEDPGRGFLPAPGTVTEFTPPTG
PGVRLDAGVESGSVIGPAWDSLLANVIVTGASREQALQRAARAVAEFRVEGMATAIPFHQ
AVVTDPDFTADPFRVHTRWIETEFVNEIKPFAPAGEADEDEAGRETIVVEVGGKRLEVSL
PSSLGMTLARTVLRRVPKPKRRASKKVRASAASGDTLASPMQGTIVKVAVEEGQEVKEGD
LIVVLEAMKMEQPLNAHRSGTVKGLTAEVGASVTSGATICDIKD
selected fasta
>putative acyl-CoA carboxylase alpha subunit [JadJ]
GTGCGCAAGGTGCTCATCGCCAACCGTGGCGAAATCGCTGTCCGTGTGGCCCGGGCGTGC
CGGGACGCGGGTATCGGCAGCGTGGCCGTGTACGCCGAGCCGGACCGGGATGCTCTGCAT
GTCCGGGCGGCTGACGAGGCGTTCGCTCTGGGCGGTGACACTCCGGCGACCAGTTATCTG
GACATGGCGAAGGTGTTGCAGGCCGCCGCGGACTCGGGTGCGGACGCGGTCCACCCCGGT
TACGGGTTCCTTTCGGAGAACGCGGAGTTCGCGCAGGCGGTCCTCGACGCGGGTCTGACG
TGGATCGGTCCGCCGCCGCAGGCGATCCGGGATCTGGGTGACAAGGTGGCGGCCCGTCAC
ATCGCCCAGCGTGCCGGGGCCCCGCTGGTGGCCGGCACGCCCGACCCGGTCTCGGGTGCG
GACGAGGTCGTGGCGTTCGCCGAGGAGCACGGTCTGCCGATCGCGATCAAGGCGGCGTTC
GGTGGCGGTGGCCGTGGTCTGAAGGTGGCGCGGACGCTGGAGGAGGTCCCGGAGCTGTAC
GACTCCGCGGTCCGTGAGGCGGTGGCGGCGTTCGGGCGGGGCGAGTGCTTCGTGGAGCGG
TACCTGGACAAGCCGCGGCACGTGGAGACGCAGTGCCTGGCCGACCAGCACGGCAACGTG
GTCGTCGTCTCGACGCGTGACTGCTCGCTGCAGCGCCGTCACCAGAAGCTGGTGGAGGAG
GCTCCGGCGCCGTTCCTGTCGGAGGCTCAGAACGCGGAGCTGTACGCGGCGTCGAAGGCG
ATCCTGAAGGAGGCCGGTTACGTCGGTGCCGGCACGGTGGAGTTCCTCGTCGGCACGGAC
GGCACGATCTCGTTCCTCGAGGTCAACACCCGTCTGCAGGTGGAGCACCCGGTGACGGAG
GAGGTCACGGGGATCGACCTGGTGCGGGAGATGTTCCGGATCGCGGACGGTGAGGAGCTC
GGCTACGGCGACCCGGAGATCCGGGGTCACTCCTTCGAGTTCCGGATCAACGGTGAGGAC
CCGGGCCGTGGTTTCCTCCCGGCGCCCGGCACCGTCACCGAGTTCACGCCGCCGACCGGT
CCGGGTGTCCGCCTCGACGCGGGTGTGGAGTCGGGTTCCGTGATCGGCCCCGCGTGGGAC
TCGCTGCTTGCGAACGTGATCGTGACCGGTGCGAGCCGTGAGCAGGCGCTGCAGCGTGCG
GCGCGTGCCGTGGCGGAGTTCCGCGTCGAGGGCATGGCGACCGCGATCCCCTTCCACCAG
GCGGTGGTGACCGACCCGGACTTCACCGCGGACCCGTTCCGGGTGCACACGCGGTGGATC
GAGACGGAGTTCGTCAACGAGATCAAGCCGTTCGCGCCGGCGGGGGAGGCGGACGAGGAC
GAGGCCGGCCGCGAGACGATCGTGGTCGAGGTCGGCGGCAAGCGTCTTGAGGTCTCCCTG
CCGTCCTCGCTCGGGATGACGCTGGCCCGGACGGTCTTGCGGCGGGTGCCGAAGCCGAAG
CGGCGGGCGTCGAAGAAGGTCCGGGCTTCGGCCGCCTCGGGTGACACCCTCGCCTCCCCG
ATGCAGGGCACGATCGTGAAGGTCGCGGTCGAGGAGGGCCAGGAGGTCAAGGAAGGCGAT
CTGATCGTCGTCCTGGAGGCCATGAAGATGGAACAGCCCCTCAACGCCCACCGCTCCGGC
ACCGTGAAGGGCCTGACCGCCGAAGTCGGCGCGTCCGTCACCTCCGGCGCCACCATCTGC
GACATCAAGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000089 Biotin/lipoyl attachment (Domain)
[515-582] PS50968
PS50968   BIOTINYL_LIPOYL
[517-582] 5.69999999999998e-17 PF00364
PF00364   Biotin_lipoyl
IPR001882 Biotin-binding site (Binding_site)
[539-556] PS00188
PS00188   BIOTIN
IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (Domain)
[285-292] PS00867
PS00867   CPSASE_2
[114-320] 2.90000000000003e-75 PF02786
PF02786   CPSase_L_D2
IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal (Domain)
[2-109] 1.40000000000001e-40 PF00289
PF00289   CPSase_L_chain
IPR005482 Biotin carboxylase, C-terminal (Domain)
[333-441] 6.29999999999998e-34 PF02785
PF02785   Biotin_carb_C
[333-441] 9.10000425645573e-54 SM00878
SM00878   Biotin_carb_C
IPR011053 Single hybrid motif (Domain)
[500-583] 2.60000517657733e-20 SSF51230
SSF51230   Hybrid_motif
IPR011054 Rudiment single hybrid motif (Domain)
[328-444] 1.40000506907309e-38 SSF51246
SSF51246   Rudmnt_hyb_motif
IPR011761 ATP-grasp fold (Domain)
[119-316] PS50975
PS50975   ATP_GRASP
IPR011764 Biotin carboxylation domain (Domain)
[1-443] PS50979
PS50979   BC
IPR013815 ATP-grasp fold, subdomain 1 (Domain)
[129-201] 6.3e-21 G3DSA:3.30.1490.20
G3DSA:3.30.1490.20   ATP_grasp_subdomain_1
IPR013816 ATP-grasp fold, subdomain 2 (Domain)
[203-444] 1.29999999999998e-87 G3DSA:3.30.470.20
G3DSA:3.30.470.20   ATP_grasp_subdomain_2
IPR016185 Pre-ATP-grasp fold (Domain)
[1-112] 8.59998776239544e-47 SSF52440
SSF52440   PreATP-grasp-like
[2-128] 7.5e-48 G3DSA:3.40.50.20
G3DSA:3.40.50.20   Pre-ATP_grasp
SignalP No significant hit
TMHMM No significant hit
Jado_00050 Jado_00050   Jado_00070 Jado_00070
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