Jado_00100 Jado_00100   Jado_00120 Jado_00120

Jado_00110 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction11,459 / 12,253 / + [in whole cluster]
5,211 / 6,005 / + [in contig]
Location11459..12253 [in whole cluster]
5211..6005 [in contig]
TypeCDS
Length795 bp (264 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productpolyketide C-9 ketoreductase
Product (GenBank)ketoreductase
GenejadE
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
[PMID:7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。

close this sectionSequence

selected fasta
>polyketide C-9 ketoreductase [ketoreductase]
MSQQEKQEKRVALVTGATSGIGLAVARLLASQNHRVFLGARNAENVAATVKQLQDEGLEV
DGTTLDVRSGEDVKAFVQAAVDRFGTVELLVNNAGRSGAGVTADIADELWNDVIDTNLNC
VFRLTREALTTGGMREKSRGRIINMASTAGKQGVVLGAPYSASKHGVVGFTKALGNELAP
TGITVNAVCPGYVETPMAQRVRQGYAAAYETSEDAILEKFQSKIPLGRYSTPEEVAGLVG
YLASDTAASITSQALNVCGGLGNF
selected fasta
>polyketide C-9 ketoreductase [ketoreductase]
ATGTCCCAGCAGGAGAAGCAGGAGAAGAGGGTCGCCCTCGTCACCGGAGCCACCAGTGGC
ATCGGCCTCGCCGTCGCCCGGCTCCTGGCGTCCCAGAACCACCGGGTGTTCCTCGGTGCC
CGCAACGCCGAGAACGTCGCCGCCACCGTCAAGCAGCTCCAGGACGAGGGCCTTGAGGTC
GACGGCACCACCCTCGACGTGCGCTCCGGCGAGGACGTCAAGGCCTTCGTCCAGGCCGCC
GTCGACCGCTTCGGCACCGTCGAGCTCCTCGTGAACAACGCGGGCCGCAGCGGCGCGGGC
GTCACCGCCGACATCGCCGACGAGCTGTGGAACGACGTCATCGACACCAACCTCAACTGC
GTCTTCCGGCTCACCCGCGAGGCCCTGACCACCGGCGGCATGCGCGAGAAGTCCCGCGGC
CGGATCATCAACATGGCCTCGACCGCCGGCAAGCAGGGCGTCGTCCTCGGCGCCCCCTAC
TCGGCCTCGAAGCACGGCGTCGTCGGCTTCACCAAGGCCCTCGGCAACGAGCTCGCCCCG
ACCGGCATCACCGTCAACGCGGTCTGCCCCGGTTACGTCGAGACGCCGATGGCCCAGCGC
GTCCGCCAGGGCTACGCCGCCGCGTACGAGACCTCCGAGGACGCCATCCTCGAGAAGTTC
CAGTCGAAGATCCCGCTCGGCCGCTACTCCACGCCCGAGGAGGTCGCCGGTCTCGTCGGC
TACCTGGCCTCCGACACCGCGGCCTCCATCACCTCGCAGGCGCTCAACGTCTGCGGCGGC
CTGGGCAACTTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[85-96] 1.69999922284049e-13 PR00080 [140-148] 1.69999922284049e-13 PR00080 [160-179] 1.69999922284049e-13 PR00080
PR00080   SDRFAMILY
[1-256] 5.09999882439978e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
[11-178] 3.60000000000002e-34 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[11-28] 9.19998414420354e-50 PR00081 [85-96] 9.19998414420354e-50 PR00081 [134-150] 9.19998414420354e-50 PR00081 [160-179] 9.19998414420354e-50 PR00081 [181-198] 9.19998414420354e-50 PR00081 [225-245] 9.19998414420354e-50 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[4-261] 5.09999999999993e-81 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[147-175] PS00061
PS00061   ADH_SHORT
SignalP
[1-30] 0.857 Signal
Bacteria, Gram-positive   
[1-26] 0.505 Signal
Bacteria, Gram-negative   
TMHMM
[12-31] Transmembrane (i-o)
Transmembrane 1   
Jado_00100 Jado_00100   Jado_00120 Jado_00120
Page top