Jado_00110 Jado_00110   Jado_00130 Jado_00130

Jado_00120 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction12,294 / 13,226 / + [in whole cluster]
6,046 / 6,978 / + [in contig]
Location12294..13226 [in whole cluster]
6046..6978 [in contig]
TypeCDS
Length933 bp (310 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productcyclase/dehydratase
Product (GenBank)bifunctional cyclase/dehydrase
GenejadD
Gene (GenBank)
EC number
Keyword
  • 1st ring
Note
Note (GenBank)
Reference
ACC
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
[PMID:7881555](1994)
ORF4(=jadDに相当)は配列解析からbifunctional cyclase/dehydraseであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadD, DpsFの両cyclasesは、C-9で還元されたdecaketidesの位置特異的なfirst-ring cyclase/dehydrasesであることが示された。

close this sectionSequence

selected fasta
>cyclase/dehydratase [bifunctional cyclase/dehydrase]
MTTREVEHEITVAAPAQAVYRLIAEVENWPRIFPPTLYVDHVERGEREERIRIWATANGA
PKNWTSKRTLDPDNLRITFRQEVSTPPVAAMATWIIEPVSATESGSALLHDYRAIDDDPE
GLKWVDEAVDRNSRSELAALKTNVELAHASEEITFSFEDTVQIDGSAKDAYDFVNEANLW
PERLPHVATVRFEEETPGLQSLEMDSRAKDGSTHTTKSYRVTFPHHRIAYKQITLPALMT
LHTGYWTFEENEDGVAASSQHTVVLNTENIARILGADATVADAREYVQTALSTNSRATLG
YAKAYAEGKR
selected fasta
>cyclase/dehydratase [bifunctional cyclase/dehydrase]
ATGACGACCCGTGAGGTCGAGCACGAGATCACCGTCGCGGCCCCGGCCCAGGCCGTGTAC
CGGCTGATCGCCGAGGTGGAGAACTGGCCGCGGATCTTCCCGCCCACCCTCTACGTCGAC
CACGTCGAGCGCGGCGAGCGGGAGGAGCGCATCCGGATCTGGGCGACCGCCAACGGCGCC
CCGAAGAACTGGACGTCCAAGCGGACCCTGGACCCGGACAACCTCCGGATCACCTTCCGC
CAGGAGGTCTCCACCCCGCCGGTCGCCGCCATGGCGACCTGGATCATCGAGCCGGTCTCC
GCGACCGAGTCCGGATCCGCCTTGCTGCACGACTACCGGGCGATCGACGACGACCCCGAG
GGCCTGAAGTGGGTCGACGAGGCCGTCGACCGCAACTCGCGCTCCGAGCTCGCCGCCCTC
AAGACCAACGTCGAACTCGCCCACGCCTCCGAGGAGATCACCTTCTCCTTCGAGGACACC
GTGCAGATCGACGGCTCCGCCAAGGACGCCTACGACTTCGTCAACGAGGCGAACCTCTGG
CCGGAGCGGCTGCCGCACGTCGCCACCGTGCGCTTCGAGGAGGAGACCCCGGGCCTGCAG
AGCCTGGAGATGGACTCCCGGGCCAAGGACGGCTCCACCCACACGACGAAGTCGTACCGG
GTGACCTTCCCGCACCACCGGATCGCCTACAAGCAGATCACCCTGCCGGCGCTCATGACC
CTGCACACCGGCTACTGGACGTTCGAGGAGAACGAGGACGGCGTCGCCGCCTCCTCGCAG
CACACGGTCGTCCTCAACACCGAGAACATCGCCCGGATCCTCGGCGCCGACGCCACCGTC
GCCGACGCCCGCGAGTACGTGCAGACGGCGCTCAGCACCAACAGCCGGGCCACCCTCGGG
TACGCCAAGGCCTACGCCGAGGGCAAGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR019587 Polyketide cyclase/dehydrase (Family)
[5-145] 2.3e-11 PF10604 [155-286] 7.4e-07 PF10604
PF10604   Polyketide_cyc2
IPR023393 START-like domain (Domain)
[5-82] 1.2e-14 G3DSA:3.30.530.20 [157-256] 1.2e-14 G3DSA:3.30.530.20
G3DSA:3.30.530.20   G3DSA:3.30.530.20
SignalP No significant hit
TMHMM No significant hit
Jado_00110 Jado_00110   Jado_00130 Jado_00130
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