Jado_00190 Jado_00190   Jado_00210 Jado_00210

Jado_00200 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction19,940 / 20,458 / + [in whole cluster]
1,847 / 2,365 / + [in contig]
Location19940..20458 [in whole cluster]
1847..2365 [in contig]
TypeCDS
Length519 bp (172 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Productjadomycins binding protein
chloramphenicol binding protein
Product (GenBank)JadX
Gene
Gene (GenBank)jadX
EC number
Keyword
Note
Note (GenBank)
  • similar to MmyY
Reference
ACC
PmId
[11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002)
[26814718] JadX is a Disparate Natural Product Binding Protein. (J Am Chem Soc. , 2016)
comment
[PMID: 11932454]
jadNの下流にある8genesの同定。

jadXの機能は不明。
jadXはjadomycin B生合成に必須ではないが、jadX破壊mutantではwildに比べて収量が10-20%減る。

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[PMID: 26814718]
JadX blocked mutantsは、chloramphenicol と jadomycin の生産レベルに影響がある。また、JadXはchloramphenicol にも jadomycin にも結合する珍しいクラスの天然物結合タンパク質である。以上から、制御またはシグナル伝達での潜在的役割が示唆されている。

close this sectionSequence

selected fasta
>jadomycins binding protein [JadX]
MTTTDLTAVTTTDGPALAAELAELRATVRELSDRAAISAVCDRYAMHLDKDRGSDDWFGA
VFTDDVHLVFPMGEYKGMDGLAAFQQMARTTFARTHHISGAYAIDLRGDEATVRAHLTAF
HVRDAAAPSAHFAIGGHYDAHVVRTPEGWRIRSFTFDLVWNAGEAPGAKGHS
selected fasta
>jadomycins binding protein [JadX]
ATGACCACCACCGACCTCACCGCCGTGACCACCACCGACGGACCGGCCCTCGCCGCCGAG
CTCGCCGAACTCCGCGCCACCGTCCGCGAGTTGTCCGACCGCGCCGCGATATCCGCGGTC
TGCGACCGCTACGCCATGCACCTCGACAAGGACCGGGGCAGCGACGACTGGTTCGGCGCG
GTGTTCACCGACGACGTCCACCTCGTCTTCCCGATGGGCGAGTACAAGGGCATGGACGGC
CTCGCCGCCTTCCAGCAGATGGCCCGCACGACCTTCGCCCGCACCCACCACATCAGCGGC
GCCTACGCGATCGACCTGCGCGGCGACGAGGCGACCGTACGGGCCCACCTCACCGCCTTC
CACGTCCGCGACGCCGCCGCGCCCTCCGCCCACTTCGCCATCGGCGGGCACTACGACGCC
CACGTCGTCCGCACCCCCGAGGGCTGGCGCATCCGCTCCTTCACCTTCGACCTGGTGTGG
AACGCCGGCGAGGCCCCCGGCGCGAAGGGCCACTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-39] 0.051 Signal
Eukaryota   
[1-35] 0.052 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Jado_00190 Jado_00190   Jado_00210 Jado_00210
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