Jado_00240 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ISP5230 (=NBRC 13096)
Entry name	Jadomycin B
Contig	AY026363
Start / Stop / Direction	23,966 / 25,156 / + [in whole cluster] 5,873 / 7,063 / + [in contig]
Location	23966..25156 [in whole cluster] 5873..7063 [in contig]
Type	CDS
Length	1,191 bp (396 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glycosyltransferase
Product (GenBank)	glycosyltransferase
Gene
Gene (GenBank)	jadS
EC number
Keyword	L-digitoxose
Note
Note (GenBank)	JadS
Reference	ACC Q939Q6 PmId [11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002) [17570722] Stereochemical integrity of oxazolone ring-containing jadomycins. (Chembiochem. , 2007) comment [PIMD: 11932454](2002) jadNの下流にある8genesの同定。 jadO, P, Q, S, T, U and Vは不活化による蓄積産物解析により、jadomycin Bのdideoxysugar部分の形成と、それのaglyconeへの付着を媒介することが示された。この結果と配列類似から、sugar生合成経路を提唱。 JadSは、jadomycin aglyconへ活性型L-digitoxoseを移動するglycosyltransferaseであるとされている。各mutantsは蓄積産物としてjadomycin aglyconeしか蓄積しないので、glycosylationはjadomycin B生合成経路における必須の最終反応であると示唆される。 -- [PMID:17570722](2007) abstract precursor-directed biosynthesis studiesにより、glycosyltransferase JadSの受容体基質特異性での柔軟性が示された。
Related Reference	ACC Q9F2F9 NITE Ello_00180 PmId [11306350] Identification of a sugar flexible glycosyltransferase from Streptomyces olivaceus, the producer of the antitumor polyketide elloramycin. (Chem Biol. , 2001) [19233921] Modulation of deoxysugar transfer by the elloramycin glycosyltransferase ElmGT through site-directed mutagenesis. (J Bacteriol. , 2009) comment Blast 10th, id35%, 1e-31 Streptomyces olivaceus_elmgt Elloramycin glycosyltransferase -- [PMID:11306350](2001) sugar flexible glycosyltransferase gene(elmGT)の同定。 S.albusでelmGTを異種性発現すると、自然な糖基質L-rhamnoseに加えて、異なるmonosaccharides(both L- and D-sugars) and disaccharideを elloramycin aglycone 8-DMTCへ移動できた。 -- [PMID:19233921](2009) L309 and N312におけるSite-directed mutagenesisにより、基質特異性がもっと正確になる。

Sequence

>putative glycosyltransferase [glycosyltransferase]
MRYLFTTIPGTSHTLPLVPLAHAALAAGHEVAFAASGPALRAANAAGLQTIAAAGDEAAE
PYEELIAKVTTSDLRRVPGPKILPYVSGIFGEVGARLVEGVAEAARTWRADAVVFPPNHV
AGLLAARMTGLPAVLHGIGTPRPVFAPALAYLEPVAARSASDLPAPVADVEIDLNPASLT
APSLGGPGGGGPAAAHRLPMRYTSYNGGAEIPPGLLGRGERPRVAVTLAPSPRCTARAPC
LREIVDGSADLGIELVITTGGAELPALTGSLPPHVTCVDWVPLRTLLASCDAIVHHGGMG
STFTAFDAGVPQLAIPLTGPESVSNGRVAADRGTGIVLDPPLSVPLTAATVKSSLHELLS
NPAHRTAAAEVAAEMREMPAPAATLVQLNALLGGTA

>putative glycosyltransferase [glycosyltransferase]
ATGCGATATCTGTTCACCACCATCCCGGGCACGTCCCACACGCTGCCCCTGGTCCCGCTC
GCCCACGCGGCGCTCGCCGCCGGGCACGAGGTCGCCTTCGCCGCCTCGGGCCCCGCCCTG
CGCGCGGCCAACGCCGCCGGTCTCCAGACCATCGCGGCCGCGGGCGACGAGGCCGCGGAG
CCGTACGAGGAGCTGATCGCCAAGGTCACCACGAGCGACCTGCGCAGAGTTCCCGGCCCG
AAGATCCTCCCGTACGTCTCCGGGATCTTCGGCGAGGTCGGCGCGCGTCTGGTGGAGGGC
GTCGCAGAGGCGGCCCGCACCTGGCGGGCCGACGCCGTGGTCTTCCCGCCCAACCACGTC
GCAGGACTGCTCGCCGCCCGGATGACCGGCCTGCCCGCCGTGCTGCACGGCATCGGCACC
CCCCGCCCGGTCTTCGCCCCGGCCCTCGCGTACCTGGAGCCGGTCGCCGCCCGCTCGGCG
TCTGACCTCCCGGCCCCGGTCGCCGACGTGGAGATCGACCTCAACCCGGCCTCCCTCACC
GCGCCGTCCCTCGGCGGCCCCGGCGGCGGCGGACCGGCCGCCGCGCACCGGCTCCCGATG
CGCTACACCTCGTACAACGGCGGCGCGGAGATCCCGCCCGGCCTGCTCGGCCGCGGCGAG
CGGCCCCGGGTCGCCGTGACGCTGGCTCCCTCGCCGCGCTGTACGGCGAGGGCACCATGC
CTCCGCGAGATCGTCGACGGCTCGGCGGACCTCGGCATCGAACTGGTCATCACCACCGGC
GGCGCCGAACTGCCCGCCCTCACCGGCTCGTTGCCCCCGCACGTCACCTGCGTGGACTGG
GTCCCGCTGCGCACCCTGCTCGCGAGCTGCGACGCGATCGTCCACCACGGCGGCATGGGC
AGCACCTTCACCGCCTTCGACGCCGGGGTCCCGCAGCTGGCGATCCCGCTCACCGGCCCG
GAGAGCGTCTCCAACGGCCGGGTCGCCGCCGACCGCGGCACCGGCATCGTCCTCGACCCG
CCGCTGTCCGTCCCGCTCACCGCCGCGACCGTGAAGTCCTCGCTGCACGAGCTGCTCAGC
AACCCCGCGCACCGCACGGCGGCCGCCGAAGTCGCCGCCGAGATGCGGGAGATGCCGGCG
CCCGCCGCCACCCTGGTCCAGCTCAACGCGCTCCTCGGAGGGACGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive Eukaryota
TMHMM	No significant hit

Jado_00230 Jado_00250