Jado_00230 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ISP5230 (=NBRC 13096)
Entry name	Jadomycin B
Contig	AY026363
Start / Stop / Direction	23,016 / 23,915 / + [in whole cluster] 4,923 / 5,822 / + [in contig]
Location	23016..23915 [in whole cluster] 4923..5822 [in contig]
Type	CDS
Length	900 bp (299 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	NDP-glucose phosphate nucleotidyltransferase
Gene
Gene (GenBank)	jadQ
EC number	2.7.7.24
Keyword	L-digitoxose
Note
Note (GenBank)	JadQ
Reference	ACC Q939Q7 PmId [11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002) comment jadNの下流にある8genesの同定。 jadO, P, Q, S, T, U and Vは不活化による蓄積産物解析により、jadomycin Bのdideoxysugar部分の形成と、それのaglyconeへの付着を媒介することが示された。この結果と配列類似から、sugar生合成経路を提唱。 JadQ(glucose-1-phosphate nucleotidyltransferase)は、NDP派生体へとglucoseを活性化すると割り当てられている。
Related Reference	ACC Q9AGY4 PmId [12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002) comment Blast 30th, id63%, 1e-104 Aneurinibacillus thermoaerophilus_rmlA Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24) 活性測定あり

Sequence

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose phosphate nucleotidyltransferase]
MRLGTPEERTVKGIFLAGGHGTRLHPITLGTSKQMLPVYDKPMIYYPLSVLMLAGIRDIQ
IISSPDDIENFRRLLGDGSPLGISLSYAVQEQPRGLAEAFLISADHIGDDSVALVLGDNI
FHGPGFAGILQDKAVDVDGCVLFGYPVRDPERYGVGEVDADGRLVSLEEKPEHPRSDLAI
TGLYFYDNDVIDIAKNLTPSARGELEITDVNRIYLERGKAELVSLGRGFVWLDAGTHDAL
TEAGQYVQILEHRQGVRLACLEEIAWRMGFIDREACLRLGEELSKSPYGQYVMEIARAG

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose phosphate nucleotidyltransferase]
GTGAGGCTGGGAACCCCAGAGGAGAGAACTGTGAAAGGCATCTTCCTGGCCGGAGGCCAC
GGCACCCGGCTGCACCCGATCACCCTCGGCACGTCCAAGCAGATGCTGCCGGTCTACGAC
AAGCCGATGATCTACTACCCGCTCTCGGTGCTGATGCTCGCCGGGATCCGGGACATCCAG
ATCATCTCGTCCCCCGACGACATCGAGAACTTCCGCCGGCTGCTCGGCGACGGCTCCCCG
CTCGGCATCTCGCTCAGCTACGCCGTCCAGGAGCAGCCCCGCGGCCTCGCCGAGGCCTTC
CTCATCTCCGCCGACCACATCGGGGACGACTCCGTCGCCCTCGTCCTCGGCGACAACATC
TTCCACGGCCCCGGCTTCGCCGGAATCCTCCAGGACAAGGCCGTGGACGTGGACGGCTGC
GTGCTCTTCGGCTACCCGGTCCGCGACCCCGAGCGCTACGGCGTCGGCGAGGTCGACGCG
GACGGCCGGCTGGTCTCCCTGGAGGAGAAGCCGGAGCACCCGCGCTCCGACCTCGCCATC
ACCGGCCTCTACTTCTACGACAACGACGTCATCGACATCGCCAAGAACCTCACGCCCTCG
GCGCGCGGCGAACTGGAGATCACCGACGTCAACCGGATCTACCTGGAGCGCGGCAAGGCC
GAACTGGTGTCCCTGGGACGCGGGTTCGTCTGGCTCGACGCCGGAACGCACGACGCGCTG
ACCGAGGCCGGCCAGTACGTGCAGATCCTGGAGCACCGCCAGGGCGTCCGGCTCGCCTGC
CTCGAGGAGATCGCCTGGCGCATGGGGTTCATCGACCGGGAGGCCTGCCTGCGGCTCGGC
GAGGAGCTCTCGAAGTCCCCGTACGGCCAGTACGTCATGGAGATAGCCCGGGCCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family) TIGR01207 RmlA
SignalP	No significant hit
TMHMM	No significant hit

Jado_00220 Jado_00240