Jado_00220 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ISP5230 (=NBRC 13096)
Entry name	Jadomycin B
Contig	AY026363
Start / Stop / Direction	21,912 / 22,910 / + [in whole cluster] 3,819 / 4,817 / + [in contig]
Location	21912..22910 [in whole cluster] 3819..4817 [in contig]
Type	CDS
Length	999 bp (332 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-3 ketoreductase
Product (GenBank)	NDP-hexose 3-ketoreductase
Gene
Gene (GenBank)	jadP
EC number
Keyword	L-digitoxose
Note
Note (GenBank)	JadP
Reference	ACC Q939Q8 PmId [11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002) comment jadNの下流にある8genesの同定。 jadO, P, Q, S, T, U and Vは不活化による蓄積産物解析により、jadomycin Bのdideoxysugar部分の形成と、それのaglyconeへの付着を媒介することが示された。この結果と配列類似から、sugar生合成経路を提唱。 JadO(NDP-hexose 2,3-dehydratase)、JadP(oxidoreductase)はNDP-2,6-dideoxy-D-threo-4-hexuloseを産生する反応が割り当てられている。
Related Reference	ACC Q9ALN5 NITE Spino_00080 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 13th, id49%, 3e-68 Saccharopolyspora spinosa_spnN Probable NDP-hexose-3-ketoreductase Forosamine合成過程で、3位のケト基の還元に関与する。 -- NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

Sequence

>putative NDP-hexose C-3 ketoreductase [NDP-hexose 3-ketoreductase]
MLGCADIAVRRMMPAMAALPGTEVTAVASRDAAKAAVAAAPYGAAAVEGYQELLRRPDVD
AVYVPLPAALHAEWTEAALRAGKHVLAEKPLTTDPESTAALLGLAAASGLALMETSCSST
TASTPTSRKLVADGAIGELRALHACSASPGCPTPTFRHDPGLGGGALGDVGVYPLRAAQH
LLGDELDVLGAHLVRGPGSRVETAGGALLATPAGVTAHIAFGMDHGYRSAYELWGSQGRL
VVERAYTPPADHRPVVRLETRTGVEALQKWVQGWFNLVLDADDQVRNTVAAFAALVARTG
AGHPGDGGAPDPEAPLRQARLLDAVRRRAALV

>putative NDP-hexose C-3 ketoreductase [NDP-hexose 3-ketoreductase]
GTGCTCGGCTGCGCCGACATCGCCGTCCGGCGCATGATGCCCGCGATGGCCGCGCTCCCC
GGGACCGAGGTCACCGCCGTCGCCAGCCGCGACGCGGCGAAGGCCGCCGTGGCCGCCGCC
CCGTACGGCGCGGCGGCCGTCGAGGGCTACCAGGAGCTGCTGCGCCGCCCGGACGTCGAC
GCCGTCTACGTACCGCTGCCCGCCGCCCTGCACGCCGAGTGGACCGAGGCCGCCCTGCGG
GCCGGCAAGCACGTCCTGGCCGAGAAGCCGCTCACCACGGACCCGGAGAGCACCGCCGCG
CTGCTCGGCCTGGCCGCCGCGTCCGGGCTCGCGCTGATGGAGACGTCATGTTCGTCCACC
ACCGCCTCCACACCGACGTCCCGCAAGCTCGTCGCGGACGGAGCCATCGGCGAACTCCGC
GCCCTGCACGCCTGTTCGGCATCCCCCGGCTGCCCGACACCGACATTCCGGCACGACCCC
GGGCTCGGCGGCGGCGCCCTCGGCGACGTCGGCGTCTACCCGCTGCGAGCCGCCCAGCAC
CTGCTCGGCGACGAACTCGACGTCCTCGGCGCGCACCTCGTCCGGGGGCCCGGCAGCCGG
GTCGAGACCGCCGGCGGGGCGCTGCTCGCCACCCCGGCGGGCGTCACCGCGCACATCGCC
TTCGGCATGGACCACGGCTACCGCTCGGCGTACGAGCTGTGGGGCAGCCAGGGCCGGCTC
GTCGTCGAGCGGGCCTACACCCCGCCCGCCGACCACCGCCCGGTGGTGCGCCTGGAGACC
CGCACCGGCGTCGAGGCGCTTCAGAAGTGGGTTCAAGGATGGTTCAACCTCGTCCTCGAC
GCCGACGACCAGGTCCGCAACACGGTGGCCGCCTTCGCCGCCCTCGTGGCGCGCACCGGA
GCCGGCCACCCCGGGGACGGCGGCGCGCCCGACCCGGAGGCCCCGCTGCGCCAGGCGCGG
CTGCTCGACGCCGTGCGGCGCCGGGCCGCCCTCGTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000683 Oxidoreductase, N-terminal (Domain) PF01408 GFO_IDH_MocA IPR004104 Oxidoreductase, C-terminal (Domain) PF02894 GFO_IDH_MocA_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Jado_00210 Jado_00230