Jado_00250 Jado_00250   Jado_00270 Jado_00270

Jado_00260 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction26,196 / 26,789 / + [in whole cluster]
8,103 / 8,696 / + [in contig]
Location26196..26789 [in whole cluster]
8103..8696 [in contig]
TypeCDS
Length594 bp (197 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)NDP-4-keto-6-deoxyhexose 5-epimerase
Gene
Gene (GenBank)jadU
EC number5.1.3.-
Keyword
  • L-digitoxose
Note
Note (GenBank)
  • JadU
Reference
ACC
PmId
[11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002)
comment
jadNの下流にある8genesの同定。

jadO, P, Q, S, T, U and Vは不活化による蓄積産物解析により、jadomycin Bのdideoxysugar部分の形成と、それのaglyconeへの付着を媒介することが示された。
この結果と配列類似から、sugar生合成経路を提唱。

JadU(NDP-4-keto-2,6-dideoxy-5-epimerase)は中間体NDP-2,6-dideoxy-D-threo-4-hexuloseをL-erythro型へ変換するとされている。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 14th, id49%, 2e-43
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [NDP-4-keto-6-deoxyhexose 5-epimerase]
MQVTPLAIEGAFAFTPPVFKDSRGLFASPYQGDVFAGQLGRPLFQVNQVSHNLSARGTLR
GVHFTATPPGMAKYVYCPYGRLRDFLIDLRVGSPTFGQWVMNELDAETSRALYVPVGVGH
AFVSLQDDSMCVYVMSQGYSPPTNWPCTRSTRRSDLPAGRRRADPVRPDKIAPTLAEARD
RGLLPDFDTCRKLEELL
selected fasta
>putative NDP-hexose epimerase [NDP-4-keto-6-deoxyhexose 5-epimerase]
ATGCAGGTGACCCCCCTGGCCATCGAGGGCGCCTTCGCCTTCACGCCCCCCGTCTTCAAG
GACAGCCGCGGCCTGTTCGCCTCCCCCTACCAGGGGGACGTGTTCGCCGGGCAGCTCGGC
CGCCCGCTCTTCCAGGTCAATCAGGTCAGCCACAACCTGTCCGCCAGGGGCACCCTCCGC
GGCGTCCACTTCACCGCGACCCCGCCGGGCATGGCGAAGTACGTCTACTGCCCGTACGGC
AGGCTCCGGGACTTCCTCATCGACCTCCGGGTCGGCTCACCGACCTTCGGGCAGTGGGTG
ATGAACGAACTCGACGCGGAGACCAGCCGCGCCCTCTACGTCCCGGTCGGCGTCGGCCAC
GCGTTCGTCTCGCTCCAGGACGACTCCATGTGCGTATACGTGATGTCGCAGGGGTACTCC
CCGCCAACGAACTGGCCCTGCACCCGCTCGACCCGGCGCTCGGACTTACCTGCCGGACGA
CGTCGAGCTGATCCTGTCCGACCGGACAAGATCGCCCCCACCCTCGCCGAGGCCCGCGAC
CGCGGCCTCCTGCCCGACTTCGACACCTGCCGCAAGCTGGAGGAACTGCTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[4-143] 1.5e-40 PF00908
PF00908   dTDP_sugar_isom
[1-133] PD001462
PD001462   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[1-182] 1.99999636034521e-50 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[1-149] 1.2e-44 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP
[1-13] 0.345 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Jado_00250 Jado_00250   Jado_00270 Jado_00270
Page top