Jado_00260 Jado_00260   Jado_00280 Jado_00280

Jado_00270 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction26,828 / 27,805 / + [in whole cluster]
8,735 / 9,712 / + [in contig]
Location26828..27805 [in whole cluster]
8735..9712 [in contig]
TypeCDS
Length978 bp (325 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 ketoreductase
Product (GenBank)NDP-4-keto-6-deoxyhexose 4-ketoreductase
Gene
Gene (GenBank)jadV
EC number
Keyword
  • L-digitoxose
Note
Note (GenBank)
  • JadV
Reference
ACC
PmId
[11932454] Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone. (Microbiology. , 2002)
comment
jadNの下流にある8genesの同定。

jadO, P, Q, S, T, U and Vは不活化による蓄積産物解析により、jadomycin Bのdideoxysugar部分の形成と、それのaglyconeへの付着を媒介することが示された。
この結果と配列類似から、sugar生合成経路を提唱。

JadV(NDP-4-keto-2,6-dideoxyhexose 4-ketoreductase)はNDP-4-hexuloseのketo基を還元するとされている。
Related Reference
ACC
Q9F834
NITE
Megalo_00080
PmId
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
Blast 5th, id55%, 2e-74
Micromonospora megalomicea subsp. nigra_megDV
TDP-4-keto-6-deoxyhexose 4-ketoreductase

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。
MegDVがmegosamine生合成に特異的に関与する4-ketoreductaseであることを確認。

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-4-keto-6-deoxyhexose 4-ketoreductase]
MVVLGAAGFVGSAVLRELARHDLRVRAVSRRPVTVPAGARAEVETRSVDLTVPGAMAGAI
EGADVVVHSIAYIAGSSTWRITDGDTGAERVNVGLVRDLVEAVRASGRPDTAVLFTGSVS
AAGPSERGVYDGTEPDEPQGEYDRQKLAAERVLLAASAEGVLRGISLRLPTVFGYGPDST
ARDKGIVSSMVRRAVAGEPITMWHDGTVRRDLLYVEDVARAIAAALGHLDALAGRHWVLG
SGHGAELGPVFTTVARMVAERTGKPPVAVRSVEPPAHAEARDFASVTVDPRAFHEVTGWR
AEVPLDEALDRTTAFSASGAEEHFG
selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-4-keto-6-deoxyhexose 4-ketoreductase]
GTGGTCGTCCTCGGCGCCGCCGGCTTCGTCGGCTCCGCCGTCCTGCGCGAACTCGCCCGC
CACGACCTGCGGGTGCGGGCCGTCTCCCGGCGCCCGGTGACCGTCCCGGCCGGCGCCCGC
GCCGAGGTGGAGACCCGCAGCGTCGACCTCACCGTCCCCGGCGCGATGGCCGGGGCGATC
GAGGGCGCCGACGTGGTCGTCCACTCGATCGCCTACATCGCGGGCTCCTCGACCTGGCGC
ATCACGGACGGCGACACCGGCGCCGAGCGGGTCAACGTGGGACTCGTACGGGACCTGGTC
GAGGCCGTGCGGGCGAGCGGCCGCCCCGACACCGCCGTCCTGTTCACCGGCTCCGTCTCC
GCCGCCGGACCCTCGGAGCGGGGCGTCTACGACGGTACGGAGCCGGACGAGCCGCAGGGC
GAGTACGACCGGCAGAAGCTGGCGGCCGAGCGGGTGCTGCTCGCGGCGAGCGCCGAGGGC
GTGCTGCGCGGGATCTCGCTCCGGCTCCCCACGGTCTTCGGCTACGGCCCCGACTCGACC
GCGCGGGACAAGGGCATCGTCTCCTCGATGGTCCGCCGGGCGGTGGCGGGCGAGCCGATC
ACCATGTGGCACGACGGCACGGTCCGCCGCGACCTGCTGTACGTGGAGGACGTGGCGCGC
GCTATCGCGGCGGCCCTCGGCCACCTCGACGCGCTCGCGGGACGGCACTGGGTGCTCGGC
AGCGGGCACGGCGCCGAGCTCGGCCCGGTCTTCACCACGGTCGCCCGGATGGTGGCGGAG
CGGACCGGCAAGCCGCCGGTGGCCGTGCGCTCGGTGGAGCCGCCCGCGCACGCCGAGGCC
CGGGACTTCGCCAGCGTCACCGTCGACCCGCGCGCCTTCCACGAGGTCACCGGCTGGCGG
GCCGAGGTCCCGCTGGACGAGGCCCTGGACCGGACGACGGCCTTCAGCGCGAGCGGCGCG
GAGGAGCACTTCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[1-240] 1.5e-48 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[2-221] 1.5e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-41] 0.296 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Jado_00260 Jado_00260   Jado_00280 Jado_00280
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