Kirro_00310 : CDS information

Location

Organism	Streptomyces collinus
Strain	Tü365
Entry name	Kirromycin
Contig	AM746336
Start / Stop / Direction	91,056 / 92,255 / + [in whole cluster] 91,056 / 92,255 / + [in contig]
Location	91056..92255 [in whole cluster] 91056..92255 [in contig]
Type	CDS
Length	1,200 bp (399 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450 hydroxylase
Gene
Gene (GenBank)	kirOI
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B0B511 PmId [18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008) comment kirromycin生合成gene clusterの報告。 KirOI: Cytochrome P450 hydroxylase 相同性より名付けられている。 KirOIとKirOIIが、C-16とC-30のhydroxylationに直接関与するかどうか、あるいは、tetrahydrofuran環の形成に関与するかを検証するためのmutation実験が今後行われると記述有り。
Related Reference	ACC A6YRR3 PmId [19376895] Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14. (Appl Environ Microbiol. , 2009) comment Streptomyces virginiae Cytochrome P450 monooxygenase 105 cytochrome P450 monooxygenase, FcpCを同定した文献。 FcpCがdiosgenoneのC-25のhydroxylationを担い、isonuatigenoneに変換する機能を持つことがin vitroで示されている。この論文で同定されたfcpC(1191nt) and fcpD(243nt)遺伝子配列のGenBank accession numberはEF646279であると記載あり。よってこのentry=FcpCで合っている。

Sequence

>putative cytochrome P450 [putative cytochrome P450 hydroxylase]
MSETVRPVDGLPMTRGTCPFDPAPELAELREEQPVARMVFPDGHLGWLITGYDEVRRLLA
ARGMSSRGDLLRTPIPLPMAGNRTELAPGMFTAMDPPEHTHYRRRLTAWFSARRTRTMEP
RLTEHVDLYLGRMIEEGGPTDLVAAFAEPVAGLVICELLGVPADRRDVFVKGIKALLTVH
SSAEEAIAGWQNVGGQLMELIRAKREEPTDDLLGTLVSDGAFSDEELATIGSVLLVGGYD
TSKNMIALGTFALLAHPDQYAALAADPGLGAGAVEELLRYVTVMHAGSIRAAGADMDFDG
HHFTEGDAVSLSLAAANRDPSLCEDPDRLDITRPPVAHLSFGYGIHQCVGQQLARLELRI
AFEGLARRLPGLRLAIPEDQVRTNPESIIYGVHELPVTW

>putative cytochrome P450 [putative cytochrome P450 hydroxylase]
GTGTCCGAGACCGTTCGTCCCGTCGACGGCCTGCCCATGACCCGTGGCACGTGCCCGTTC
GACCCCGCACCCGAGCTCGCCGAGCTGCGCGAGGAGCAGCCGGTGGCCCGCATGGTCTTC
CCGGACGGCCACCTCGGCTGGCTGATCACCGGCTACGACGAGGTCCGCCGGCTCCTCGCC
GCCCGGGGCATGAGCTCGCGCGGCGACCTGCTGCGCACGCCGATCCCGCTGCCGATGGCC
GGGAACCGGACCGAACTGGCGCCGGGGATGTTCACGGCGATGGACCCGCCGGAGCACACC
CACTACCGGCGCCGGCTGACCGCCTGGTTCTCGGCCCGCCGCACCCGGACGATGGAGCCG
AGGCTGACCGAGCACGTCGACCTGTATCTCGGCAGGATGATCGAGGAAGGCGGACCCACC
GACCTGGTCGCCGCGTTCGCCGAGCCGGTCGCCGGGCTGGTGATCTGCGAGCTGCTCGGT
GTTCCCGCCGACCGGCGGGACGTGTTCGTCAAGGGCATCAAGGCGCTGCTGACCGTCCAC
TCCAGCGCGGAGGAGGCGATCGCCGGCTGGCAGAACGTGGGCGGGCAGCTGATGGAGCTG
ATCCGCGCCAAACGCGAGGAGCCCACCGACGACCTGCTCGGCACGCTGGTCTCCGACGGC
GCTTTCAGCGACGAGGAGCTGGCGACCATCGGCAGCGTGCTGCTCGTGGGCGGCTACGAC
ACCAGCAAGAACATGATCGCGCTGGGCACCTTCGCCCTGCTGGCACACCCCGACCAGTAC
GCGGCGCTGGCCGCGGACCCGGGCCTGGGCGCGGGCGCGGTGGAGGAACTGCTGCGTTAC
GTGACGGTCATGCACGCCGGCTCGATCCGCGCGGCCGGCGCCGACATGGACTTCGACGGG
CACCACTTCACCGAGGGAGACGCCGTGTCCCTGTCGCTGGCCGCGGCCAACCGGGACCCG
TCGCTCTGCGAGGACCCGGACCGCCTGGACATCACCCGGCCGCCGGTGGCGCACCTGTCG
TTCGGTTACGGCATCCACCAGTGCGTCGGCCAGCAACTGGCCCGCCTTGAGCTGCGGATC
GCGTTCGAGGGGCTGGCCCGGCGGCTGCCCGGGCTGCGGCTGGCGATCCCCGAGGACCAG
GTCCGCACCAACCCCGAATCGATCATCTACGGCGTCCACGAGCTGCCGGTGACATGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Kirro_00300 Kirro_00320