Kirro_00440 : CDS information

Location

Organism	Streptomyces collinus
Strain	Tü365
Entry name	Kirromycin
Contig	AM746336
Start / Stop / Direction	100,994 / 102,361 / + [in whole cluster] 100,994 / 102,361 / + [in contig]
Location	100994..102361 [in whole cluster] 100994..102361 [in contig]
Type	CDS
Length	1,368 bp (455 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative crotonyl-CoA reductase
Product (GenBank)	putative crotonyl-CoA reductase
Gene
Gene (GenBank)	kirN
EC number	1.3.1.-
Keyword	ethylmalonyl-CoA
Note
Note (GenBank)
Reference	ACC B0B524 PmId [18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008) comment kirromycin生合成gene clusterの報告。相同性から機能付けしている。 KirNは前駆体butyryl-CoAを十分量生産するために要求され、引き続きethylmalonyl-CoAのカルボキシル化を引き起こすと予測している。
Related Reference	ACC Q53865 PmId [8521864] Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. (Eur J Biochem. , 1995) comment Streptomyces collinus Crotonyl CoA reductase(ccr) 分子量からおそらくhomodimerで機能していると考えられる。 E.coli でcloning､発現､精製し、活性をみている。crotonyl-CoAとenoyl-CoA（C4）には高い基質特異性を見せるが、enoyl- CoA thioester, acryloyl-CoAのような短鎖(C3)には測定可能な活性はみられなかった。
	ACC Q9RNU6 PmId [10542184] Role of crotonyl coenzyme A reductase in determining the ratio of polyketides monensin A and monensin B produced by Streptomyces cinnamonensis. (J Bacteriol. , 1999) comment Streptomyces cinnamonensis Crotonyl-CoA reductase(ccr) S. cinnamonensis_ccr、そのS. cinnamonensisで異種性発現させたS. collinus_ccr、ccr-disrupted mutantなどでCCR activity測定あり。 monensin A/monensin Bの比率は、CCR活性のレベルと、CCRの基質であるcrotonyl-CoAの両方に依存する。
	ACC B8XVS5 PmId [17548827] Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. (Proc Natl Acad Sci U S A. , 2007) [19458256] Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase. (Proc Natl Acad Sci U S A. , 2009) comment Rhodobacter sphaeroides Crotonyl-CoA carboxylase/reductase(ccr) この酵素だけでcrotonyl-CoAのreductionとreductive carboxylationが触媒されることを確かめている。 crotonyl-CoA＋NADPH＋CO2 → ethylmalonyl-CoA-＋NADP+

Sequence

>putative crotonyl-CoA reductase [putative crotonyl-CoA reductase]
MQDIIDAVLDPDTRPEDFAALPVPESYRAVTLHADEVEMFVRVAAEDRDPRRSMHLDEVA
VPQVGPGEALVAVMAGSINYNTVWSALFEPLPTFTFLARYGRRSPLARRHDLPYHVIGSD
LSGVVLRTGPGVFAWNPGDEVVAHCLSAELEAPDGHSDTMLDPDQRIWGYETNFGGLAQL
ALVKANQLMPKPAHLTWEEAGTPGLVASTAYRQLVSRNGARLKQGDNVLIWGASGGVGAF
ATQFALAGGANPICVVSSPAKAAICRAMGADAIIDRADEGYTFWKDEYTQDPAEWKRFGR
RIRELTGGEDPDIVFEHPGRDTFGASVYVTRKGGTIVTCASTSGYRHEYDNRYLWMSLKR
IVGSHFANYREAWESNRLIAKGRIHPTLSRTYTLEHAGQAVDDVYRNRHLGKVGVLALAP
HEGLGVRDTAMRERHLDAINRFRQTNRTPTGKGNK

>putative crotonyl-CoA reductase [putative crotonyl-CoA reductase]
ATGCAAGACATCATCGACGCCGTTCTCGACCCGGACACCCGGCCGGAGGACTTCGCCGCG
CTGCCCGTCCCGGAGAGCTACCGCGCCGTGACCCTGCACGCGGACGAGGTCGAGATGTTC
GTCCGGGTCGCGGCCGAGGACCGCGATCCGCGCCGGTCGATGCACCTCGACGAAGTCGCC
GTGCCCCAGGTCGGGCCCGGCGAGGCGCTGGTGGCGGTGATGGCCGGCTCGATCAACTAC
AACACCGTGTGGTCCGCGCTCTTCGAACCGCTGCCGACGTTCACGTTCCTGGCCCGGTAC
GGCCGCCGCTCGCCCCTGGCCCGGCGGCACGACCTCCCCTACCACGTCATCGGCTCCGAC
CTCTCCGGAGTCGTGCTGCGTACCGGGCCCGGCGTGTTCGCGTGGAACCCCGGCGACGAG
GTCGTCGCGCACTGCCTGTCGGCCGAGTTGGAGGCGCCCGACGGGCACAGCGACACGATG
CTCGATCCGGACCAGCGCATCTGGGGGTACGAGACCAACTTCGGCGGCCTGGCACAACTG
GCGCTGGTCAAGGCGAACCAGCTGATGCCCAAACCGGCGCACCTGACCTGGGAGGAGGCC
GGCACACCCGGTCTGGTCGCCTCCACCGCGTACCGGCAACTGGTCTCCCGCAACGGTGCC
CGGCTGAAGCAGGGCGACAACGTGCTCATCTGGGGCGCGTCCGGCGGCGTGGGGGCGTTC
GCGACGCAGTTCGCGCTGGCCGGCGGCGCCAACCCGATCTGTGTGGTGTCCAGCCCGGCC
AAGGCCGCCATCTGCCGGGCGATGGGCGCCGACGCGATCATCGACCGTGCCGACGAGGGC
TACACCTTCTGGAAGGACGAGTACACCCAGGACCCGGCGGAATGGAAACGGTTCGGCCGC
CGGATCCGGGAGCTCACGGGTGGGGAGGATCCCGACATCGTCTTCGAGCATCCCGGCCGG
GACACCTTCGGCGCCTCGGTGTACGTCACCCGCAAGGGCGGCACCATCGTCACCTGTGCC
TCCACCTCCGGCTACCGCCACGAGTACGACAACCGCTACCTGTGGATGTCGCTGAAGCGG
ATCGTCGGATCGCATTTCGCCAACTACCGGGAGGCATGGGAGTCCAACCGGCTCATAGCC
AAGGGCAGGATCCACCCCACCCTCTCCCGCACGTACACCCTGGAACACGCCGGTCAGGCA
GTCGACGACGTGTACCGCAACCGGCACCTCGGCAAGGTCGGCGTGCTGGCCCTGGCACCG
CACGAGGGGCTGGGCGTCCGTGACACCGCGATGCGCGAGCGGCATCTCGACGCGATCAAC
CGCTTCCGGCAGACGAACCGCACGCCTACAGGGAAGGGAAACAAATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010085 Crotonyl-CoA reductase (Family) TIGR01751 Crot-CoA-red IPR011032 GroES-like (Domain) SSF50129 GroES_like IPR013149 Alcohol dehydrogenase, C-terminal (Domain) PF00107 ADH_zinc_N IPR013154 Alcohol dehydrogenase GroES-like (Domain) PF08240 ADH_N IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Kirro_00430 Kirro_00450