Kirro_00450 Kirro_00450   Kirro_00470 Kirro_00470

Kirro_00460 : CDS information

close this sectionLocation

Organism
StrainTü365
Entry nameKirromycin
Contig
Start / Stop / Direction103,763 / 103,254 / - [in whole cluster]
103,763 / 103,254 / - [in contig]
Locationcomplement(103254..103763) [in whole cluster]
complement(103254..103763) [in contig]
TypeCDS
Length510 bp (169 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative acetyltransferase
Product (GenBank)putative GNAT family acetyltransferase
Gene
Gene (GenBank)kirE
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008)
comment
kirromycin生合成gene clusterの報告。

kirE: Acetyltransferase, GNAT family

GCN5-related N-acetyltransferase domainを持つORF。
同じdomainを持つPKSの機能解析より、PKS loading moduleのACPへのacetyl-CoA starter unitのloadingに関与しているかもしれないと考察している。

このORFについて機能解析実験はされていない。
Family/Domain
FD
IPR000182
comment
[IPR000182] GCN5-related N-acetyltransferase (Domain)

close this sectionSequence

selected fasta
>putative acetyltransferase [putative GNAT family acetyltransferase]
MPDVSVRLANTADRPVVERLWLMFRHDMSELGNLLPNADGTFRSDRLHMAFSEPDWAPYL
LTSGDRPAGFAFVRGLTAPTRVLNSFFVVRGARRTGIGLHAVQEIAARHPGSWEVAFQDA
NIAAVHFWRRVATEISDDAWTEERRPIPGRPELPPDVWISFNAPGETDG
selected fasta
>putative acetyltransferase [putative GNAT family acetyltransferase]
GTGCCAGACGTATCCGTTCGTCTCGCGAACACCGCCGACCGCCCCGTGGTGGAGCGGCTC
TGGCTGATGTTCCGCCATGACATGTCGGAGTTGGGCAATCTGCTGCCGAACGCTGACGGA
ACCTTCCGCAGCGACCGGCTCCACATGGCCTTCTCCGAGCCCGACTGGGCGCCGTACCTC
CTCACAAGCGGTGACCGACCGGCCGGCTTCGCGTTCGTCCGCGGTCTGACCGCCCCGACA
CGGGTGCTGAACAGCTTCTTCGTGGTGCGTGGTGCACGGCGGACAGGGATCGGGCTGCAC
GCTGTTCAGGAGATCGCGGCCAGACACCCAGGCTCGTGGGAAGTCGCGTTCCAGGATGCC
AACATCGCCGCTGTGCACTTCTGGCGTCGCGTCGCCACGGAGATCTCCGACGACGCGTGG
ACCGAGGAACGCCGACCGATCCCAGGGCGGCCCGAACTGCCTCCTGATGTCTGGATCTCG
TTCAACGCCCCTGGGGAGACGGACGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000182 GNAT domain (Domain)
[18-164] PS51186
PS51186   GNAT
IPR016181 Acyl-CoA N-acyltransferase (Domain)
[1-130] 4.5e-07 G3DSA:3.40.630.30
G3DSA:3.40.630.30   Acyl_CoA_acyltransferase
[3-130] 2.19999900980708e-10 SSF55729
SSF55729   Acyl_CoA_acyltransferase
SignalP No significant hit
TMHMM No significant hit
Kirro_00450 Kirro_00450   Kirro_00470 Kirro_00470
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