Lando_00040 Lando_00040   Lando_00060 Lando_00060

Lando_00050 : CDS information

close this sectionLocation

Organism
StrainS136
Entry nameLandomycin
Contig
Start / Stop / Direction5,135 / 5,404 / + [in whole cluster]
5,135 / 5,404 / + [in contig]
Location5135..5404 [in whole cluster]
5135..5404 [in contig]
TypeCDS
Length270 bp (89 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein homolog
Gene
Gene (GenBank)lanC
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • LanC
Reference
ACC
PmId
[9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999)
comment
landomycin生合成gene clusterの同定論文。

lanC: acyl carrier protein

配列解析のみ。
urdamycin clusterのUrdC, jadomycin clusterのOrf3(JadC)に似ている。
Related Reference
ACC
Q56165
NITE
Jado_00100
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 5th, id71%, 2e-26
Streptomyces venezuelae_acp(jadC)
Acyl carrier protein

--
[PMID:7881555](1994)
ORF3(=JadC)は配列解析からtype II PKSのACPであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadCもACPとして使用されている。

close this sectionPKS/NRPS Module

ACP7..80

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein homolog]
MSQPEFTVEDLKRILVEGAGADEGVDLDSDILDTDFESLGYESLALLETAGRIEREFGIT
LDDEVFIDNPTPRELVAAVNAKLPGSLAA
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein homolog]
ATGTCTCAGCCCGAGTTCACCGTCGAGGACCTCAAGCGCATCCTGGTGGAGGGCGCGGGC
GCCGACGAGGGCGTCGACCTCGACAGCGACATCCTCGACACCGACTTCGAGAGCCTCGGT
TACGAGTCGCTGGCCCTCCTGGAGACTGCCGGGCGCATCGAGCGCGAGTTCGGCATCACC
CTCGACGACGAGGTCTTCATCGACAACCCCACGCCGCGTGAGCTGGTGGCGGCCGTCAAC
GCGAAGCTGCCCGGCTCCCTCGCCGCCTGA
ACP7..80
ACP19..240

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[38-53] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[5-84] 2.5e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[7-80] PS50075
PS50075   ACP_DOMAIN
[26-79] 2.3e-12 PF00550
PF00550   PP-binding
[4-86] 2.39999798157265e-17 SSF47336
SSF47336   ACP_like
SignalP No significant hit
TMHMM No significant hit
Lando_00040 Lando_00040   Lando_00060 Lando_00060
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