Lando_00160 : CDS information

Location

Organism	Streptomyces cyanogenus
Strain	S136
Entry name	Landomycin
Contig	AF080235
Start / Stop / Direction	16,318 / 17,727 / + [in whole cluster] 16,318 / 17,727 / + [in contig]
Location	16318..17727 [in whole cluster] 16318..17727 [in contig]
Type	CDS
Length	1,410 bp (469 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	NDP-hexose 2,3-dehydratase homolog
Gene
Gene (GenBank)	lanS
EC number
Keyword	D-olivose L-rhodinose
Note
Note (GenBank)	LanS
Reference	ACC Q9ZGC4 PmId [9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999) comment landomycin生合成gene clusterの同定論文。 lanS: NDP-hexose 2,3-dehydratase 配列解析のみ。 L-daunosamineのC-2 deoxygenationで2,3-dehydrataseとして関連するかもしれないS. peucetius_DnmTに似ている。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 34th, id51%, 1e-125 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [NDP-hexose 2,3-dehydratase homolog]
MLSSLVRTGSGTGRLRPRHDPSVAERIAASAAAVTGASLRTEDFPQWLEGRRRAHRFTVD
RIPFDALDGWSFDDATGNLVHRSGRFFSVEGLHVTRDEGPHREWYQPIIKQPEVGILGIL
VKEFDGVLHFLMQAKMEPGNRNLLQLSPTVQATRSNYTKVHKGADVKYIEYFTQPGRGRF
IADVLQSEHGSWFFHKSNRNMIVEAVGDVPLDDDFCWLTLGQIGQLLHRDNVVNMDSRTV
LACAPFPDDGADALLSDTELVSWFTAERSRHDVRAERVPLHDLPGWVRGTSTIDHELGRY
FRVVAVSVEAGSREVTGWTQPLFEPLGLGVTAFLTRRIGGVPHVLVHARVEGGFLDTVEL
GPTVQYTPDNYGHLTGEDRPPFLDLVLDADPARIQYEAVHSEEGGRFLNAESRYLLIEAD
EEQAPLDPPAGFQWVTPAQLTSLVRHGHYVNVQARTLLACLNATAVLSG

>putative NDP-hexose 2,3-dehydratase [NDP-hexose 2,3-dehydratase homolog]
GTGCTTTCCTCCCTTGTCAGGACTGGATCCGGGACCGGTCGGCTGCGGCCGCGCCACGAC
CCCTCGGTCGCCGAGCGCATCGCCGCGTCGGCCGCCGCCGTCACGGGCGCGAGCCTGCGC
ACCGAGGACTTCCCGCAGTGGCTCGAAGGGCGCCGAAGGGCCCATCGGTTCACCGTGGAC
CGGATCCCCTTCGATGCGCTCGACGGCTGGTCCTTCGACGACGCCACCGGCAACCTGGTG
CACCGCAGCGGCCGGTTCTTCTCCGTCGAGGGACTGCACGTCACCCGGGACGAAGGGCCG
CACCGCGAGTGGTACCAGCCCATCATCAAGCAGCCCGAGGTCGGGATCCTCGGCATTCTC
GTCAAGGAGTTCGACGGGGTGCTGCACTTCCTCATGCAGGCCAAGATGGAGCCGGGCAAC
CGCAACCTGCTGCAGCTCTCGCCGACCGTGCAGGCCACCCGGAGCAACTACACCAAGGTC
CACAAGGGCGCCGATGTGAAGTACATCGAGTACTTCACCCAGCCCGGCCGCGGGCGGTTC
ATCGCCGATGTGCTGCAGTCCGAGCACGGATCGTGGTTCTTCCACAAGAGCAACCGCAAC
ATGATCGTCGAGGCCGTCGGCGACGTGCCGCTCGACGACGACTTCTGCTGGCTCACCCTC
GGCCAGATAGGCCAGCTCCTGCACCGCGACAATGTCGTGAACATGGACTCGCGCACGGTC
CTCGCCTGCGCTCCGTTCCCCGACGACGGGGCGGACGCCCTGCTCTCGGACACCGAACTC
GTCTCGTGGTTCACCGCCGAGCGCTCCCGGCACGACGTGCGCGCCGAGCGCGTCCCGCTC
CACGACCTCCCCGGCTGGGTCCGCGGCACGTCGACCATCGATCACGAGCTGGGCCGGTAC
TTCCGCGTCGTCGCCGTCTCCGTGGAGGCGGGCAGCCGCGAGGTCACCGGCTGGACCCAG
CCCCTGTTCGAGCCCCTTGGCCTCGGCGTCACCGCCTTCCTGACCCGGCGGATCGGCGGC
GTGCCGCACGTCCTGGTGCACGCGCGCGTGGAGGGCGGCTTCCTCGACACCGTCGAGCTC
GGCCCCACCGTCCAGTACACGCCGGACAACTACGGGCACCTGACCGGCGAGGACCGGCCG
CCCTTCCTCGACCTGGTCCTCGACGCCGACCCGGCCCGCATCCAGTACGAGGCCGTCCAC
TCGGAGGAAGGCGGCCGCTTCCTCAACGCGGAGAGCCGATACCTTCTGATCGAGGCCGAC
GAGGAGCAGGCCCCGTTGGACCCGCCCGCCGGTTTCCAGTGGGTCACGCCCGCCCAGCTC
ACTTCCCTCGTACGGCACGGCCACTACGTGAACGTCCAGGCCCGAACCCTGCTCGCCTGC
CTCAACGCGACGGCGGTGCTCTCCGGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Lando_00150 Lando_00170