Lando_00220 : CDS information

Location

Organism	Streptomyces cyanogenus
Strain	S136
Entry name	Landomycin
Contig	AF080235
Start / Stop / Direction	22,417 / 23,589 / + [in whole cluster] 22,417 / 23,589 / + [in contig]
Location	22417..23589 [in whole cluster] 22417..23589 [in contig]
Type	CDS
Length	1,173 bp (390 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	D-olivose glycosyltransferase
Product (GenBank)	glycosyl transferase homolog
Gene
Gene (GenBank)	lanGT1
EC number
Keyword	2nd D-olivose 5th D-olivose
Note
Note (GenBank)	LanGT1
Reference	ACC Q9ZGB8 PmId [9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999) [11755402] Elucidation of the function of two glycosyltransferase genes (lanGT1 and lanGT4) involved in landomycin biosynthesis and generation of new oligosaccharide antibiotics. (Chem Biol. , 2001) [16039521] Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A. (Chem Biol. , 2005) comment [PMID:9933932](1999) landomycin生合成gene clusterの同定論文。 lanGT1: glycosyltransferase 配列解析のみ。 --- [PMID:11755402](2001) landomycinとurdamycinのglycosyltransferase gene cassettesで発現実験、産物解析。 LanGT1は、他のD-olivoseの4-OH基へ2つ目のD-olivoseを結合するD-olivosyltransferaseである。 LanGT4がD-olivoseの3-OH基にL-rhodinoseを付着するL-rhodinosyltransferaseである。 LanGT4の基質特異性はゆるい。 --- [PMID:16039521](2005) landomycin E(糖側鎖がtrisaccharide: -D-olivose-D-olivose-L-rhodinose)は、wild株でlandomycin Aへ変換されるが、lanGT1 mutantではlandomycin I (tetrasaccharide: -D-olivose-D-olivose-L-rhodinose-D-olivose)を蓄積。これは5番目の糖の移動がLanGT1の不在下で起こることができないことを示す。したがって、LanGT1はこれまでに示されている2番目に加えて、5番目のglycosyltransferも触媒する。 - Significanceでは2番目と4番目と書いてあるが、内容を確認すると4番目→5番目の間違い。また、同著者らの後発の論文ではlandomycin I はD-olivoseひとつのみを持ち、このtetrasaccharideを持つものはlandomycin Jとされている。

Sequence

>D-olivose glycosyltransferase [glycosyl transferase homolog]
MRVLMINTPVSTHLTPLVPLAWALRAAGHEVLVLGREDVLGAAASAGLNAVSIGDWLKLD
DILVERLGGKRPLQSWGRPALDQLSNVGSLWMPQTLEVLPAYLDLARDFGPDLVLSDPIE
FISLIVGEDLGVPVVQQRWGVDPISDQVRGAGETWFKDILAERGLTGLPRPAVLLDPCPP
SLQLAGVTPGTPMRYVPFNGNGRLPEWLRRDARERGTARRVAVSLGGRTLAYNGVPLMRN
ILDALGSLPDVEVLATVEEEYREALGEVPGNVRVIDPVPLHLLLGSCHAVVHHGGSGTAM
TASAFGLPQLVLPQLADQFWHGDALARVGAAITIESVERQDEPEPVREAAAALLGDPAYE
KAAGELRAEIAAMPAPSRVVTDLEKLVAAR

>D-olivose glycosyltransferase [glycosyl transferase homolog]
ATGCGCGTCCTCATGATCAACACACCCGTTTCGACTCATCTCACCCCGCTGGTCCCGCTC
GCGTGGGCGCTGCGCGCGGCAGGGCACGAGGTGCTGGTGCTCGGCCGCGAGGACGTCCTC
GGCGCGGCCGCGTCAGCGGGCCTGAACGCCGTGAGCATCGGCGACTGGCTCAAGCTGGAC
GACATACTGGTCGAGCGGCTCGGCGGCAAGCGGCCTTTGCAGTCCTGGGGCAGGCCGGCC
CTGGACCAGCTCTCCAACGTCGGCTCCCTGTGGATGCCCCAGACGCTGGAGGTACTGCCC
GCGTACCTGGACCTGGCCCGCGACTTCGGACCCGACCTGGTTCTCTCCGACCCGATCGAG
TTCATCTCGCTGATCGTGGGGGAGGATCTCGGCGTCCCCGTGGTGCAGCAGCGCTGGGGT
GTCGACCCCATCTCGGACCAGGTCCGCGGCGCGGGCGAGACCTGGTTCAAGGACATCCTC
GCGGAGCGGGGCCTGACCGGCCTGCCGCGCCCGGCCGTCCTGCTCGACCCGTGCCCGCCC
AGCCTCCAGCTGGCCGGCGTGACTCCCGGCACCCCGATGCGCTATGTGCCCTTCAACGGC
AACGGCCGACTGCCCGAGTGGCTCCGGCGTGATGCCCGGGAGCGCGGAACGGCCCGGCGC
GTCGCCGTCTCTCTGGGAGGGCGCACTCTCGCGTACAACGGTGTCCCCCTCATGCGGAAC
ATCCTGGACGCGCTCGGCTCGCTGCCGGACGTCGAAGTGCTGGCGACCGTGGAGGAGGAG
TACCGCGAGGCGCTCGGTGAGGTCCCCGGCAACGTACGCGTCATCGATCCCGTCCCGCTA
CACCTGCTGCTCGGCTCCTGCCACGCCGTCGTCCACCACGGCGGCTCGGGCACGGCCATG
ACCGCGAGCGCCTTCGGCCTGCCGCAGCTCGTCCTGCCCCAGCTGGCGGACCAGTTCTGG
CACGGCGACGCTCTGGCTCGGGTGGGCGCGGCCATCACCATCGAGAGTGTCGAGCGGCAG
GACGAGCCGGAGCCGGTGCGCGAGGCAGCGGCCGCGCTGCTCGGCGACCCGGCCTACGAG
AAGGCGGCGGGGGAACTGCGCGCGGAAATCGCCGCGATGCCGGCCCCGTCTCGGGTCGTG
ACCGACCTGGAGAAGCTGGTCGCGGCCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-positive Eukaryota
TMHMM	No significant hit

Lando_00210 Lando_00230