Lando_00240 Lando_00240   Lando_00260 Lando_00260

Lando_00250 : CDS information

close this sectionLocation

Organism
StrainS136
Entry nameLandomycin
Contig
Start / Stop / Direction26,033 / 26,611 / + [in whole cluster]
26,033 / 26,611 / + [in contig]
Location26033..26611 [in whole cluster]
26033..26611 [in contig]
TypeCDS
Length579 bp (192 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)NDP-hexose 3,5-epimerase homolog
Gene
Gene (GenBank)lanZ1
EC number5.1.3.-
Keyword
  • L-rhodinose
Note
Note (GenBank)
  • LanZ1
Reference
ACC
PmId
[9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999)
comment
landomycin生合成gene clusterの同定論文。

lanZ1: NDP-hexose 3,5-epimerase

配列解析のみ。
S. peucetius_DnmUに似ている。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 22th, id50%, 7e-40
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [NDP-hexose 3,5-epimerase homolog]
MQAVEAKVPGVHVLAPWTGSKDERGAFFESLRTDLLQDVVGHPFEVKQINYSVSRRNHLR
GIHSVTSPPGQAKYVSCVRGAFRDFVVDLRVGSPTFGQYDVNLLDAASGRALYIPEGVGH
GFLTLTEDACICYVLSSTYVPGTQIDIDPLDPDLALPWGFTEPPLISEKDRTARSLAATL
EAGLLSEWPGGN
selected fasta
>putative NDP-hexose epimerase [NDP-hexose 3,5-epimerase homolog]
GTGCAGGCAGTCGAGGCGAAAGTGCCGGGTGTCCACGTCCTGGCACCGTGGACCGGATCC
AAGGACGAGCGCGGTGCCTTCTTCGAGTCGCTGCGCACCGACCTGCTCCAGGACGTCGTG
GGGCACCCCTTCGAAGTGAAGCAGATCAACTACTCGGTGTCTCGCCGCAACCACCTGCGG
GGGATCCACAGCGTCACCAGCCCGCCGGGGCAGGCCAAGTACGTCTCCTGCGTCCGCGGC
GCGTTCCGTGACTTCGTCGTCGACCTGCGGGTGGGCTCGCCGACCTTCGGGCAGTACGAC
GTCAACCTCCTCGATGCCGCATCGGGCCGCGCCCTCTACATCCCGGAGGGCGTGGGGCAC
GGCTTCCTCACGCTCACCGAGGACGCCTGCATCTGCTACGTCCTGTCGAGCACGTACGTC
CCCGGCACCCAGATCGACATCGACCCCCTCGACCCGGATCTCGCCCTGCCGTGGGGCTTC
ACCGAGCCGCCGCTGATCTCCGAGAAGGACAGGACGGCGCGTTCGCTCGCCGCCACCCTG
GAGGCCGGACTCCTCTCGGAGTGGCCCGGCGGCAACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[1-177] PD001462
PD001462   dTDP_sugar_isom
[5-177] 2.19999999999997e-46 PF00908
PF00908   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[1-183] 8.20001307135067e-62 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[1-183] 9.69999999999989e-55 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP No significant hit
TMHMM No significant hit
Lando_00240 Lando_00240   Lando_00260 Lando_00260
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