Lasal_00110 Lasal_00110   Lasal_00130 Lasal_00130

Lasal_00120 : CDS information

close this sectionLocation

Organism
StrainATCC 35851
Entry nameLasalocid
Contig
Start / Stop / Direction14,560 / 12,833 / - [in whole cluster]
14,560 / 12,833 / - [in contig]
Locationcomplement(12833..14560) [in whole cluster]
complement(12833..14560) [in contig]
TypeCDS
Length1,728 bp (575 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative 3-hydroxyacyl-CoA dehydrogenase
putative beta-keto-reductase
Product (GenBank)putative 3-hydroxybutyryl-CoA dehydrogenase
Gene
Gene (GenBank)lsd10
EC number1.1.1.35
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
Reference
ACC
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S.lasaliensis ATCC35851のLasalocid生合成gene clusterの報告。

lsd10: ethylmalonyl-CoA biosynthesis
homolog : putative oxidoreductase_S.kanamyceticus

lasalocid 生合成のlate stepにおいてepoxidase以外にredox enzymeがないため、lsd9,lsd10はethylmalonyl-CoA生合成に対して働くと推測している。しかし、lsd10のホモログであるplmT8で提唱されているC-8 hydroxyethyl側鎖のC-8 hydroxyl基をamineで置換する機能と相違があるとも記載有り。
Related Reference
ACC
B5M9K7
NITE
Lasal2_00150
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
comment
GenBank,Uniprotでは、NRRL 3382Rと登録されている。

S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

las6: 3-hydroxybutyryl CoA dehydrogenase
homolog: PlmT8_Streptomyces sp. HK803

このORFとlas5は、3-hydroxyacyl CoA dehydrogenaseとcrotonyl-CoA reductaseをエンコードすると推測された。両ORFは、PKSのためのunusual butyrate unitsの原料であるethylmalonyl CoAの供給に関与しそうだと推測している。
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
comment
Clostridium acetobutylicum
3-hydroxybutyryl-CoA dehydrogenase_hbd

BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス。
crt geneの上流にあるプロモーターによって制御される。transcriptional unitであることを確認。

BCS operonをE. coliで過剰発現。
crotonase, beta-hydroxybutyryl-CoA dehydrogenase activity確認。

BCS operonをC. acetobutylicum で発現。
crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activity上昇。

close this sectionSequence

selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [putative 3-hydroxybutyryl-CoA dehydrogenase]
MGMAQQRFPTVAVVGLGITGSALMTLLARTGHRVVAVDVDEAALERARDRARAAGVEGVD
FTTRFADVARADLLVEAVPEQLEAKRQVLRRAHEVCPGATVFATTTTSLSVTGLAFESGR
MASTIGLHFFPTAPENGLGAVELIATPVTARALLDDVRELLHGLGSTAVSSTDSPGFVGG
ALTMSYLNGAAAMYEQRYAAEEGIDAAMTLGCGVPLGPLAQLDAMGLDTAHDTLEALYQR
TGDRGYAPAPVLTHMVAAGLLGVKSGRGFHDYAGGRAAGDARTGAQAAVPRTVRTIGVVG
SGVMATGIAEVCARSGHPTTLVARSGVKAKEALAAVGRSLERQVRRGRRTPEELAATTAR
LSGASAYEALADCDLVVEAVAEDLAVKRGVFGALDRVCRPGAVLATSTSSLPVVECATAT
GRPQDVLGMHFFNPAPVMKLVEVVRTVLTAQDTVSTALAAAAALGKRPVQCADRAGFIVN
ALLFPYLNRAVCMMREGVATADEIDTVMTAGHGFPMGPFKLLDVIGLDVSLAIQKTLHRA
FREPALAPARPLEQLVEAGYLGRKAGRGFRSYDTV
selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [putative 3-hydroxybutyryl-CoA dehydrogenase]
ATGGGCATGGCACAGCAGCGTTTCCCGACGGTTGCGGTCGTCGGGCTCGGCATAACGGGT
TCCGCCCTCATGACATTACTGGCCCGTACCGGACACCGGGTGGTCGCGGTCGACGTGGAC
GAGGCGGCTCTGGAGCGCGCCCGGGACCGGGCTCGCGCGGCGGGAGTGGAGGGTGTCGAC
TTCACCACGCGGTTCGCCGACGTCGCCCGGGCCGATCTTCTCGTCGAGGCGGTGCCCGAA
CAGCTGGAGGCCAAGCGGCAGGTCCTGCGCCGCGCCCACGAAGTGTGCCCGGGCGCCACC
GTGTTCGCGACCACCACGACGTCCCTGTCGGTGACCGGTCTCGCCTTCGAGTCCGGGCGG
ATGGCGAGCACGATCGGCCTGCACTTCTTTCCCACGGCCCCTGAAAACGGCCTCGGAGCC
GTGGAGTTGATCGCCACCCCGGTCACGGCCCGGGCGTTGCTGGACGACGTCCGCGAGCTG
CTGCACGGCCTCGGCAGCACCGCCGTGTCCAGCACCGACAGCCCCGGTTTCGTCGGCGGC
GCCCTGACGATGTCGTACCTGAACGGCGCCGCCGCCATGTACGAGCAGCGCTACGCCGCC
GAGGAGGGCATCGACGCCGCGATGACCCTGGGCTGCGGCGTGCCCCTCGGCCCGCTCGCG
CAGCTCGACGCGATGGGCCTGGACACCGCCCACGACACCCTGGAAGCGCTCTACCAGCGC
ACCGGGGACCGCGGTTACGCCCCGGCCCCCGTGCTGACGCACATGGTGGCCGCCGGGCTG
CTCGGCGTGAAGTCGGGGCGCGGCTTCCACGACTACGCGGGCGGACGGGCGGCCGGCGAC
GCCCGCACAGGAGCGCAGGCGGCCGTGCCGCGCACCGTCCGCACCATCGGCGTGGTCGGC
TCCGGCGTCATGGCCACCGGCATCGCCGAGGTGTGCGCGCGCTCCGGACACCCCACCACG
CTGGTCGCCCGCAGCGGGGTGAAGGCGAAGGAGGCCCTGGCCGCGGTCGGGCGCTCACTG
GAGCGGCAGGTCCGCCGCGGCAGGCGGACCCCCGAGGAACTCGCCGCGACCACCGCCCGG
CTCAGCGGCGCGTCGGCCTACGAGGCGCTTGCCGACTGCGACCTCGTGGTGGAGGCCGTC
GCCGAGGACCTCGCGGTCAAACGGGGGGTGTTCGGCGCACTGGACCGGGTGTGCCGGCCC
GGCGCCGTGCTCGCCACCTCCACCTCCAGCCTGCCCGTCGTCGAATGCGCCACGGCCACC
GGTCGCCCGCAGGACGTGCTGGGCATGCACTTCTTCAACCCGGCACCGGTCATGAAGCTC
GTGGAGGTCGTACGGACGGTGCTGACCGCCCAGGACACGGTGTCCACCGCCCTCGCGGCC
GCCGCCGCCCTCGGCAAGCGGCCCGTCCAGTGCGCGGACCGGGCCGGCTTCATCGTGAAC
GCCCTGCTCTTCCCTTACCTCAACCGGGCGGTGTGCATGATGCGCGAGGGGGTGGCCACC
GCCGACGAGATCGACACGGTGATGACGGCCGGCCACGGCTTCCCGATGGGCCCCTTCAAG
CTGCTGGACGTGATCGGCCTCGACGTCTCACTGGCCATCCAGAAGACCCTGCACCGCGCG
TTCCGCGAGCCGGCGCTCGCCCCTGCCCGCCCGCTGGAACAGCTGGTGGAGGCGGGTTAC
TTGGGACGCAAGGCGGGACGCGGCTTCCGCTCGTACGACACCGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
[176-272] 5.30000000000001e-23 PF00725 [476-572] 1.4e-29 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
[10-173] 6.29999999999998e-33 PF02737 [295-473] 5.19999999999996e-57 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[175-276] 4.09999599045784e-25 SSF48179 [476-573] 1.29999924468179e-29 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
[178-272] 5.40000000000002e-56 G3DSA:1.10.1040.10 [478-572] 5.40000000000002e-56 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
[8-177] 5.20000000000001e-81 G3DSA:3.40.50.720 [290-477] 5.20000000000001e-81 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-36] 0.689 Signal
Eukaryota   
[1-36] 0.862 Signal
Bacteria, Gram-positive   
[1-36] 0.329 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Lasal_00110 Lasal_00110   Lasal_00130 Lasal_00130
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