Lasalocid : Cluster information
Compound
| Entry name | Lasalocid |
|---|---|
| PKS Type | TypeI modular |
| Starter Unit | malonyl-CoA |
| Chain Length | 12 |
| Sugar Unit | none |
| Classification | Polyether |
| Activity | Antibacterial Ionophore |
| Composition | A: C34H54O8 |
Original source
| Organism | Streptomyces lasaliensis |
|---|---|
| Strain | ATCC 35851 |
| Contig | AB449340 |
PKS/NRPS Module
| Lasal_00130 lsd11 | 0 |  | malonyl-CoA |
| 1 |  | ethylmalonyl-CoA | |
| 2 |  | malonyl-CoA | |
| Lasal_00140 lsd12 | 3 | | ethylmalonyl-CoA |
| 4 | | methylmalonyl-CoA | |
| 5 |  | ethylmalonyl-CoA | |
| Lasal_00150 lsd13 | 6 |  | methylmalonyl-CoA |
| Lasal_00160 lsd14 | 7 | | methylmalonyl-CoA |
| Lasal_00170 lsd15 | 8 | | malonyl-CoA |
| 9 | | malonyl-CoA | |
| Lasal_00180 lsd16 | 10 |  | methylmalonyl-CoA |
| Lasal_00190 lsd17 | 11 |  | malonyl-CoA |
Reference
- Epoxide hydrolase Lsd19 for polyether formation in the biosynthesis of lasalocid A: direct experimental evidence on polyene-polyepoxide hypothesis in polyether biosynthesis.
- Shichijo Y, Migita A, Oguri H, Watanabe M, Tokiwano T, Watanabe K, Oikawa H[PMID: 18710235]J Am Chem Soc. 130 (2008) 12230-1
- Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis.
- Migita A, Watanabe M, Hirose Y, Watanabe K, Tokiwano T, Kinashi H, Oikawa H[PMID: 19129623]Biosci Biotechnol Biochem. 73 (2009) 169-76
- Intriguing substrate tolerance of epoxide hydrolase Lsd19 involved in biosynthesis of the ionophore antibiotic lasalocid A.
- Matsuura Y, Shichijo Y, Minami A, Migita A, Oguri H, Watanabe M, Tokiwano T, Watanabe K, Oikawa H[PMID: 20394359]Org Lett. 12 (2010) 2226-9
- Enzymatic epoxide-opening cascades catalyzed by a pair of epoxide hydrolases in the ionophore polyether biosynthesis.
- Minami A, Migita A, Inada D, Hotta K, Watanabe K, Oguri H, Oikawa H[PMID: 21375229]Org Lett. 13 (2011) 1638-41
- Enzymatic catalysis of anti-Baldwin ring closure in polyether biosynthesis.
- Hotta K, Chen X, Paton RS, Minami A, Li H, Swaminathan K, Mathews II, Watanabe K, Oikawa H, Houk KN, Kim CY[PMID: 22388816]Nature. 483 (2012) 355-8
- Sequential enzymatic epoxidation involved in polyether lasalocid biosynthesis.
- Minami A, Shimaya M, Suzuki G, Migita A, Shinde SS, Sato K, Watanabe K, Tamura T, Oguri H, Oikawa H[PMID: 22506807]J Am Chem Soc. 134 (2012) 7246-9
- Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.
- Minami A, Oguri H, Watanabe K, Oikawa H[PMID: 23796908]Curr Opin Chem Biol. 17 (2013) 555-61
- Epoxide hydrolase-lasalocid a structure provides mechanistic insight into polyether natural product biosynthesis.
- Wong FT, Hotta K, Chen X, Fang M, Watanabe K, Kim CY[PMID: 25535803]J Am Chem Soc. 137 (2015) 86-9
Data download
| Compound name | Genomic nucleotide sequence |
Nucleotide sequences of the coding regions | Translation of the coding sequences | List of genes/ORFs | GBK | |||||||
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 Lasalocid |
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History
- 2015-02-12[Update]
- 2014-06-25[Update]
- 2013-12-27[Update]
- 2012-08-03[Update]
- 2012-06-04[Update]
- 2012-04-26[Release]
