Lasal_00180 : CDS information

Location

Organism	Streptomyces lasaliensis
Strain	ATCC 35851
Entry name	Lasalocid
Contig	AB449340
Start / Stop / Direction	65,864 / 71,578 / + [in whole cluster] 65,864 / 71,578 / + [in contig]
Location	65864..71578 [in whole cluster] 65864..71578 [in contig]
Type	CDS
Length	5,715 bp (1,904 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	putative polyketide synthase
Gene
Gene (GenBank)	lsd16
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B6ZK69 PmId [19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009) comment GenBank,Uniprotでは、ATCC31180と登録されている。 S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。 lsd16: polyketide biosynthesis, module 10 module10(KS, AT, dh, KR, ACP) module10のDH-KR domainはE-olefinを提供し、C2-C7の環化はほとんど起こらないと推測。DH10はactive siteを保存しているがinactiveでundehydrated productを産生するだろうと推定している。
Related Reference	ACC B5M9L3 NITE Lasal2_00210 PmId [19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008) [22025396] Insights into lasalocid A ring formation by chemical chain termination in vivo. (Angew Chem Int Ed Engl. , 2011) comment GenBank,Uniprotでは、NRRL3382Rと登録されている。 --- [PMID:19025863](2008) S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。 lasAVI: polyketide synthase KS, AT, DH, KR, ACP module10(KS, AT, DH, KR, ACP) Fig.1, scheme2で示したmodule10によって起こる二重結合はZ-olefinで示したが、このPKSのDH-KR domainはE-olefinを産生すると予測されていると述べている。 --- [PMID: 22025396](2011) carba(dethia) N-acetyl cysteamine esters 5a-c(実際に作用するのは加水分解されたactive biosynthetic probes 6a-c)を用いて、lasalocid A PKSから中間体をin vivoで放出させ、その誘導体を分離・解析している。推定されたdienes 8a-cでの3-OH基保持から、LasVI（LasAVIの書き間違いと思われる)のPKS DHは芳香環の形成に関与しないと示唆される。これは遺伝子解析に基づくこれまでの提唱と一致する。

PKS/NRPS Module

methylmalonyl-CoA

KS	58..434

AT	604..921

dh	971..1135

KR	1462..1642

ACP	1747..1817

Sequence

>polyketide synthase [putative polyketide synthase]
MSNEPNEPQDSHEPNVRSAVREGALSNEERLRDYLKWVTTDLHDTRRRLREVQDAQSEPI
AIVGMACRFPGGADSPDTFWELIAEGRDAISGMPTDRGWDLDALYDDDPSHAGTSYAREG
GFLPDAGHFDAALFGISPREALAMDPQQRLVLEASWEALERAGINPQSLRGSRTGVFVGH
VASGYGAHVTDAPDGVEGYLGTGTSASVASGRVAYTLGLEGPAVTVDTACSSSLVALHWA
EQALRARECSLAIVAGVTVLPSPLAFIEFSRQRGLAADGRCKPFSAAADGFGFAEGVGAL
LVQRLTDARREGREVLAVIRGSAVNQDGASNGLTAPNGPSQQRVIRQALTNARLTPGDID
MVEAHGTGTALGDPIEAQALLATYGQDRPQDRPLRLGSVKSNIGHTQAAAGMAGLIKTVL
AMRHGVMPRTLHLEEPTPHVDWTSGRVSLLTEQTAWPASDQTAWPASDRPRRAGVSSFGM
SGTNAHVILEEAPQPSTADDADDAADGPARQEDGSAPVPWVLSGKSPEALRAQAARLRAH
VVASPAPVADLGLSLATTRAALEHRGVILATDLTDAVQGLDALASGEPAPGVVSGSAHPG
SGVVFVFPGQGSQWTGMGRELLDSSPEFAAYIAECDAALSDFVDWSLTDVLRGTEGAPGY
DRVDVVQPALFAVMVSLARLWQHHGIHPDAVIGHSQGEIAAAHIAGALTLNDAARIVALR
SQALLPLAGLGGMTSLALPHDQALNLIQPWGQDLSIASVNGPHSTVVSGTTHALDQLHTT
CGTQGVRARRIPVDYASHSAQVESIRDTVFQAATGINPQPTTIPLYSTVTGQPIDGTRLD
ADYWYTNLRHTVRFEETVRTLLSHGHRHFIETTAHPVLTLALEETAQATTTDPTITGTLR
RDHGDLTQLHTALATAWTHGLPVDWASLPAFRSARPVALPTYAFQRERYWLEEGHGGPAD
VEAAGLASPGHPLLGAMVRPAGGDPLVLTGRLSLRTHPWLADHVALGTVLLPGAAFVELA
LRAGDEVGCECLEELTLETPLVLPERGAVRTQVVIGEADGTGCRSLHVYSSPEDETDESG
PWTRHASGVLAKQADVTPFEFAVWPPADAEPVDLDGFYEGLAEVGHGYGPVFRGLRAAWR
LGPDVYAEVALPEEVGDEAGKFGLHPALLDASMHAIGVGDFFADTERVRLPFAWSDVSLH
AVGARTLRVRLSPADTDAVALQLADATGRQVASVGSMVSRPVSPEQLPAARQSERDLFSQ
VWEPVRASGAAPDPDTDWVVLGADTGPLVAAVSATGVRTAVHGDVVDLLAALDAGGAVPA
VALLFCDPVATGDVAADARACTSAVLSEVQTWLSDSRLESSQLVVVTRGAVTTGAEAEVT
DLVQAPVWGLLRSAQSENPARFTVVDLDDRPASHATLPGAVATALAVNEPQVALRDGVVV
VPRLARTDGPAQPVVPDWDPSGTVLITGGTGTLGALFARHLVTRYGVRHLLLTSRRGAEA
PGASELVAELAESGAAAEVVACDVADRGALAGVLAGIDPDHPLRAVVHTAGVLDDGVIGT
MTPERIDWVMRPKTDAAVHLHELTREQDLTAFVLFSSIAGLFGNPGQANYAAANVFTDAL
ARHRHAQGLPALSLAWGLWADATGMTGHLDEGERRRMSRSGVVPLSAEHGLALFDAACAL
DEPCVAPVQLDPPALRGLADAGVLPGVLRGLVRATARRAADHGGGDGGAVLVRRLAGLAE
ADRHRELLDLVRTQAANVLGHASSASIDPDHAFNELGFDSLTAVELRNLLNAATGLRLPP
SLVFDYPTPTRLAEHLRAGLEPEIGAVPHVDPEEAAVREALASLSLQQLREAGLVDVLLS
LAGGDDAMAGTGSEAEEETLSIATMDVDDLVQLALDTPETEFRK

>polyketide synthase [putative polyketide synthase]
ATGTCGAATGAGCCGAACGAGCCGCAGGACTCCCACGAGCCGAACGTGAGGTCGGCCGTG
CGCGAGGGTGCGCTCTCGAACGAGGAGAGGCTCCGCGACTACCTGAAGTGGGTCACCACC
GACCTGCACGACACGCGCCGCCGGCTGCGCGAGGTCCAGGACGCCCAGAGCGAACCGATC
GCGATCGTGGGGATGGCGTGCCGGTTCCCCGGCGGCGCCGACTCCCCGGATACCTTCTGG
GAGTTGATCGCCGAGGGCCGTGACGCGATATCGGGGATGCCCACCGATCGCGGCTGGGAC
CTGGACGCCCTCTACGACGACGACCCCAGCCACGCCGGGACGTCGTACGCACGCGAGGGC
GGCTTCCTGCCCGACGCCGGTCACTTCGACGCGGCCCTCTTCGGGATCTCACCCCGTGAG
GCCCTCGCGATGGACCCGCAGCAGCGGCTCGTCCTCGAGGCATCCTGGGAGGCACTGGAG
CGGGCCGGTATCAACCCCCAGTCGCTGCGCGGCAGTCGTACCGGCGTCTTCGTCGGCCAT
GTGGCCTCCGGATACGGCGCACACGTCACGGACGCTCCCGACGGGGTGGAGGGGTACCTG
GGTACCGGCACCTCCGCCAGTGTCGCCTCGGGCCGCGTCGCCTACACCCTGGGGCTGGAA
GGCCCGGCGGTCACCGTCGACACCGCCTGTTCCTCCTCGCTGGTGGCGCTGCACTGGGCC
GAGCAGGCCCTGCGGGCCCGCGAGTGCTCGCTGGCGATCGTCGCCGGGGTGACGGTGCTG
CCGTCCCCGCTGGCGTTCATCGAGTTCAGCCGGCAGCGCGGACTCGCCGCCGACGGCCGC
TGCAAGCCCTTCTCGGCGGCGGCCGACGGCTTCGGTTTCGCGGAGGGCGTGGGCGCCCTG
CTCGTGCAACGCCTGACGGACGCGCGCCGCGAGGGCCGCGAGGTCCTGGCGGTGATCCGC
GGCTCGGCCGTGAACCAGGACGGCGCCAGCAACGGCCTCACCGCACCCAACGGCCCCTCC
CAGCAGCGGGTGATCCGCCAGGCCCTCACCAACGCCCGCCTCACGCCCGGCGACATCGAC
ATGGTGGAGGCCCACGGCACCGGCACCGCGCTCGGCGACCCGATCGAGGCGCAGGCGTTG
CTGGCGACGTACGGCCAGGACCGCCCGCAGGACCGCCCGCTGCGGCTGGGGTCCGTGAAG
TCCAACATCGGGCACACCCAGGCGGCCGCGGGCATGGCCGGGCTCATCAAGACCGTCCTC
GCGATGCGGCACGGGGTGATGCCCCGCACCCTGCACCTCGAGGAACCCACCCCTCACGTC
GACTGGACCTCAGGCCGCGTCTCCCTGCTCACCGAGCAGACGGCCTGGCCCGCGTCGGAC
CAGACGGCCTGGCCCGCGTCGGACCGTCCGCGCCGGGCCGGTGTCTCCTCCTTCGGCATG
AGCGGCACCAACGCCCACGTGATTCTCGAGGAGGCCCCGCAGCCCTCCACCGCCGACGAC
GCCGACGACGCCGCTGACGGGCCCGCTCGCCAGGAGGACGGGTCGGCGCCCGTCCCCTGG
GTCCTGTCCGGCAAGAGTCCCGAGGCCCTGCGTGCCCAGGCCGCACGCCTGCGTGCGCAC
GTGGTGGCGTCCCCCGCTCCGGTCGCCGACCTCGGCCTGTCCCTGGCGACGACGCGCGCT
GCGCTGGAACACCGTGGGGTGATCCTGGCGACGGACCTCACCGACGCCGTCCAGGGGCTG
GACGCGCTGGCGTCAGGTGAGCCCGCGCCGGGAGTCGTGTCCGGCTCCGCACATCCGGGG
TCCGGGGTGGTGTTCGTGTTCCCGGGTCAGGGTTCGCAGTGGACCGGCATGGGACGCGAA
CTCCTCGACAGCTCGCCCGAGTTCGCCGCGTACATTGCCGAGTGCGATGCCGCGCTCAGC
GACTTCGTGGACTGGTCGCTCACCGACGTCCTGCGCGGCACCGAAGGAGCCCCCGGCTAC
GACCGCGTCGACGTCGTCCAGCCCGCCCTCTTCGCCGTCATGGTCAGCCTCGCCCGCCTC
TGGCAACACCACGGCATCCACCCCGACGCCGTCATCGGCCACTCCCAAGGCGAAATCGCC
GCCGCCCACATCGCCGGAGCCCTCACCCTCAACGACGCCGCCCGCATCGTCGCCCTACGC
TCCCAAGCCCTCCTCCCCCTCGCCGGCCTCGGCGGCATGACCTCCCTCGCCCTCCCCCAC
GACCAAGCCCTCAACCTCATCCAGCCCTGGGGCCAAGACCTGTCCATCGCCTCCGTCAAC
GGACCCCACTCCACCGTCGTCTCCGGCACCACCCACGCCCTCGACCAACTCCACACCACC
TGCGGCACCCAAGGAGTTCGGGCCCGCCGCATCCCCGTCGACTACGCCTCCCACTCCGCC
CAAGTCGAAAGCATCCGCGACACCGTCTTCCAAGCAGCCACCGGCATCAACCCACAGCCG
ACCACCATCCCCCTCTACTCGACGGTCACCGGCCAACCCATCGACGGCACACGCCTCGAC
GCCGACTACTGGTACACCAACCTCCGCCACACCGTCCGCTTCGAGGAGACGGTCCGCACC
CTCCTCAGCCACGGCCACCGCCACTTCATCGAGACCACCGCCCACCCCGTCCTCACCCTC
GCCCTCGAGGAGACGGCTCAGGCCACCACCACCGACCCGACCATCACCGGCACCCTCCGC
CGCGACCACGGCGACCTCACCCAACTCCACACCGCCCTCGCCACCGCCTGGACCCACGGT
CTCCCCGTCGACTGGGCGTCGCTGCCCGCGTTCCGATCCGCCCGGCCCGTGGCGCTGCCG
ACCTACGCGTTCCAGCGCGAACGGTACTGGCTGGAGGAGGGGCACGGCGGTCCCGCGGAC
GTCGAAGCGGCGGGACTCGCCTCTCCCGGACATCCGCTGCTCGGTGCCATGGTGCGACCC
GCCGGAGGTGACCCGTTGGTCCTCACCGGGCGGCTGTCCCTGCGCACGCACCCCTGGCTG
GCGGACCACGTGGCATTGGGCACCGTACTGCTGCCGGGTGCCGCTTTCGTGGAACTGGCG
TTGCGGGCGGGCGACGAGGTCGGGTGCGAGTGCCTGGAGGAACTCACCCTCGAGACGCCG
CTGGTCCTGCCGGAGCGCGGCGCGGTGCGCACGCAGGTGGTGATCGGCGAGGCCGACGGG
ACCGGGTGCCGGTCGCTGCACGTGTACTCCTCCCCCGAGGACGAGACCGATGAGTCAGGT
CCGTGGACGCGGCACGCGAGCGGTGTGCTGGCCAAGCAGGCCGACGTGACGCCGTTCGAG
TTCGCGGTGTGGCCGCCCGCGGACGCCGAACCGGTCGATCTGGACGGCTTCTACGAGGGT
CTGGCCGAGGTGGGACACGGCTACGGGCCGGTGTTCCGTGGGCTGCGCGCGGCCTGGCGG
CTGGGGCCGGACGTCTACGCGGAGGTGGCCCTGCCGGAAGAGGTCGGGGACGAGGCGGGG
AAGTTCGGGCTGCATCCGGCGCTGCTCGACGCCTCGATGCACGCCATCGGTGTAGGGGAC
TTCTTCGCCGACACCGAGCGGGTGCGGCTGCCGTTCGCATGGAGCGACGTGTCCCTCCAT
GCCGTGGGCGCCCGGACCTTGCGGGTGCGGCTGTCGCCGGCCGACACGGACGCGGTGGCA
CTGCAGTTGGCGGACGCGACAGGCCGCCAGGTCGCGTCCGTCGGGTCCATGGTGTCGCGT
CCTGTCTCGCCCGAGCAGCTGCCCGCCGCCCGACAGTCGGAGCGGGACTTGTTCAGCCAG
GTCTGGGAGCCGGTGCGGGCTTCGGGTGCCGCGCCGGATCCGGACACGGACTGGGTCGTC
CTCGGGGCGGACACGGGCCCCTTGGTCGCGGCCGTCTCGGCGACGGGCGTACGGACGGCG
GTCCACGGTGACGTCGTAGACCTGTTGGCGGCTCTGGACGCGGGTGGGGCCGTGCCGGCC
GTGGCCTTGCTGTTCTGCGATCCGGTGGCCACCGGTGACGTGGCCGCGGACGCCCGTGCC
TGCACGTCCGCGGTGTTGAGCGAGGTGCAGACCTGGCTGTCCGACTCCCGGCTGGAGTCG
TCGCAGTTGGTCGTGGTCACGCGAGGCGCGGTCACCACTGGTGCGGAGGCCGAGGTCACC
GACCTGGTGCAGGCTCCGGTGTGGGGGCTGCTGCGGTCGGCGCAGTCCGAGAACCCCGCA
CGCTTCACGGTGGTCGACCTGGACGACCGTCCTGCCTCTCACGCGACGCTGCCGGGCGCG
GTGGCCACCGCGCTCGCCGTGAACGAGCCGCAGGTCGCCCTGCGTGACGGGGTGGTGGTC
GTGCCGCGGCTCGCCCGGACGGACGGACCGGCGCAGCCTGTGGTCCCGGACTGGGACCCT
TCCGGCACCGTGCTGATCACCGGCGGTACGGGCACCCTCGGTGCCCTCTTCGCCCGGCAT
CTGGTAACGCGGTACGGCGTGCGGCACCTGCTGCTGACCAGCCGTCGCGGCGCGGAGGCT
CCCGGGGCGTCCGAACTGGTCGCGGAGCTGGCCGAGTCGGGGGCGGCGGCCGAGGTGGTC
GCGTGCGACGTGGCCGACCGTGGGGCGCTGGCCGGCGTGCTGGCGGGCATCGACCCCGAC
CACCCGCTGCGGGCGGTCGTGCACACGGCGGGTGTGCTGGACGACGGTGTGATCGGCACC
ATGACCCCGGAGCGGATCGACTGGGTCATGCGGCCCAAGACGGACGCCGCCGTCCACCTC
CACGAACTGACGCGGGAGCAGGACCTGACGGCGTTCGTGCTGTTCTCCTCGATCGCCGGG
CTGTTCGGCAACCCCGGTCAGGCCAACTACGCGGCAGCGAACGTGTTCACGGACGCGCTG
GCCCGGCACCGCCACGCACAGGGTCTGCCGGCGCTGTCCCTCGCCTGGGGTCTGTGGGCC
GACGCGACCGGCATGACAGGGCACCTCGACGAGGGCGAACGACGGCGGATGTCCCGAAGC
GGTGTGGTGCCGCTGTCGGCCGAGCACGGGCTGGCCCTCTTCGACGCGGCCTGCGCTCTG
GACGAGCCCTGTGTGGCCCCGGTCCAGCTCGACCCGCCGGCCCTGCGCGGTCTGGCCGAT
GCCGGGGTGCTGCCCGGCGTCCTGCGCGGCCTGGTCCGCGCCACGGCGCGGCGGGCCGCG
GACCATGGCGGCGGTGACGGCGGTGCGGTGCTGGTCCGGCGGCTCGCCGGTCTGGCCGAG
GCGGACCGCCACCGCGAACTCCTCGACCTCGTGCGCACGCAGGCGGCGAACGTCCTCGGC
CACGCCTCTTCCGCCTCCATCGACCCCGACCACGCCTTCAACGAGCTCGGCTTCGACTCG
CTCACGGCCGTCGAGCTGCGCAACCTGCTCAACGCCGCCACCGGGCTGCGGCTGCCGCCC
AGCCTCGTCTTCGACTACCCGACCCCGACCCGCCTCGCCGAGCACCTGCGCGCCGGCCTG
GAACCGGAGATCGGCGCCGTACCGCACGTCGACCCGGAGGAGGCCGCCGTGAGGGAGGCG
CTGGCCTCGCTGTCCTTGCAGCAGTTGCGCGAGGCCGGACTGGTCGACGTGCTGCTGAGC
CTCGCCGGGGGCGACGACGCCATGGCCGGCACCGGGAGCGAGGCGGAGGAGGAGACGCTG
TCGATCGCCACGATGGACGTCGACGATCTCGTGCAGCTCGCGCTCGACACCCCCGAGACG
GAGTTCCGCAAATGA

[10] KS	58..434

[10] AT	604..921

[10] methylmalonyl-CoA	795..799

[10] dh	971..1135

[10] KR	1462..1642

[10] ACP	1747..1817

[10] KS	172..1302

[10] AT	1810..2763

[10] methylmalonyl-CoA	2383..2397

[10] dh	2911..3405

[10] KR	4384..4926

[10] ACP	5239..5451

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) G3DSA:1.10.1200.10 ACP_like SSF47336 ACP_like PS50075 ACP_DOMAIN PF00550 PP-binding IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking SSF101173 Polyketide_synth_docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020807 Polyketide synthase, dehydratase domain (Domain) SM00826 PKS_DH IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	No significant hit
TMHMM	No significant hit

Lasal_00170 Lasal_00190