Lasal_00180 Lasal_00180   Lasal_00200 Lasal_00200

Lasal_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 35851
Entry nameLasalocid
Contig
Start / Stop / Direction71,575 / 75,510 / + [in whole cluster]
71,575 / 75,510 / + [in contig]
Location71575..75510 [in whole cluster]
71575..75510 [in contig]
TypeCDS
Length3,936 bp (1,311 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)putative polyketide synthase
Gene
Gene (GenBank)lsd17
EC number
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。

lsd17: polyketide biosynthesis, module 11

module11(KS, AT, ACP, TE)

TE domainが存在することから、ポリケタイド鎖が切断された後ポリエーテル環形成が進行すると予想している。
Related Reference
ACC
B5M9L4
NITE
Lasal2_00220
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
comment
GenBank,Uniprotでは、NRRL3382Rと登録されている。

S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

lasAVII: polyketide synthase KS, AT, ACP, TE

integral C-terminal thioesteraseはマクロライドPKSのようにchain releaseを直接触媒すると推測している。

module11(KS, AT, ACP, TE)

close this sectionPKS/NRPS Module

11 malonyl-CoA
KS35..411
AT566..868
ACP937..1007
TE1105..1307

close this sectionSequence

selected fasta
>polyketide synthase [putative polyketide synthase]
MTTSPEKIVEALRASLSENERLRRQNQQLTTASREPVAIVGMACRFPGGVESPEQLWRLL
TEGRDAVADFPSDRGWDLDPLYDPDPDHPGTSYTSQGGFLYDAGQFDPLLFGISPREALA
MDPQQRLLLETSWEVLERAGIDPAALRGTDTGVFAGASASGYGSGAQEATEGAEGYAMTG
AATSVISGRVAYTFGLEGPAVTLDTACSSSLVALHLACQALRNGDCSLALACGVTVMATP
QVFTEFSRQRGLAADGRCKAFAAAADGTGFSEGVGVLLVERLSDARRNGHQVLAVVRGSA
VNQDGASNGLTAPNGPSQQRVIRQALANARLTPADVDAVEAHGTGTTLGDPIEAQALLAT
YGQNRTEDQPLWLGSVKSNIGHTQAAAGVAGVIKMVMALRHEVLPQTLHVDAPSPHIDWA
SGHVSLLTEELKWPGTDRPRRAGVSSFGISGTNAHVILEAAPPETPPADDEPERAAGPVP
WVLSARTRKALQSQAARLLTFTEETPDVSVAAVSSALLGTRAALDHRAVVLASDRVEFSR
RLHALASGRPASGVTTGSVRSGRLAFMFTGQGSQRLGMGHELYDTEPAFAAALEEAFAAL
DPHLPQPLRDIIFGDDPELLQQTQYAQPALFAVETALYRLLTDTYGLRPDYLLGHSIGEL
TAAHAAGVLTLPDAARLVTARGHLMQTAPTGGTMIAIQATEDEITEHLTPGVAIAALNSP
SSTVISGDTAEAEAIADRFRALGRKTSRLKVSHAFHSPHMDPVLDDFRQIAATLDYHPAR
IPVISNTTGQPAENLTSPDYWTQHIRRPVRFTDSLHHLTQQGVTTFLELGPDATLAMLAD
AHATAVLRRNSSEPQALRTALAELWVHGVPVAWPHSTDRRVDLPTYAFDHQHYWLDAAPG
TSATSVRPSVPVEAADAPPTLADRFEGLGAGERTSMAEELVRETVAVVLGFADPEDVDVS
TPFIKLGIDSLTAVELRRRLGGYTGLEIPAVVVFEHPTTHDLAEYLIEAMSAPPGATPDG
ASGAPSGVRATAAVDSLSDLFVRACGRGEFAEAHRLALGLAEYRPTFAGELDGARSPDVV
PLRRGTASPAVVCFPSFVWKPDFQQYLSLARELTEDRDVAVSGLPGFRPGEPLPASAEAL
ARALAAVVRRVAGGRRCVLLGHSSGGYVAAAVAAQLEEAGEAPAGLVLVDTPWWGSAGGL
GSQEWLPVINEMLLARGAAAGAEDSDAGDAWVTARAKYFSLDFAIAPRDVRTLLLRPAEP
LAGSAADAGWRAEWRPGTTVVEVPGNHFSMLDSAHAQACARAVEGWLATLG
selected fasta
>polyketide synthase [putative polyketide synthase]
ATGACCACATCGCCAGAGAAGATCGTCGAGGCGCTGCGTGCATCGCTGTCGGAGAACGAG
CGGCTGCGACGCCAGAACCAGCAGCTCACCACGGCGTCCCGGGAGCCGGTGGCCATCGTC
GGCATGGCCTGCCGGTTCCCCGGCGGGGTGGAGAGCCCCGAGCAGCTGTGGCGGCTGCTG
ACCGAGGGCCGGGACGCCGTCGCGGACTTCCCGAGCGACCGCGGCTGGGACCTGGACCCC
CTCTACGACCCGGATCCCGACCACCCGGGGACGTCGTACACGAGCCAGGGCGGATTCCTG
TACGACGCGGGGCAGTTCGACCCGCTGCTGTTCGGCATCTCGCCCCGCGAGGCCCTGGCG
ATGGACCCCCAGCAGCGCCTGCTGCTGGAGACCTCCTGGGAAGTCCTCGAACGCGCCGGC
ATCGATCCGGCCGCCCTGCGTGGCACTGACACCGGAGTCTTCGCCGGCGCCTCCGCGTCG
GGTTACGGCAGTGGGGCCCAGGAGGCCACGGAGGGGGCCGAGGGGTACGCGATGACCGGT
GCGGCCACCAGCGTCATCTCCGGCCGTGTCGCGTACACCTTCGGCCTGGAGGGCCCCGCG
GTGACGCTGGACACCGCGTGCTCCTCCTCCCTGGTCGCCCTGCACCTGGCCTGCCAGGCC
CTGCGCAACGGCGACTGCTCACTCGCGCTGGCGTGCGGTGTCACGGTGATGGCGACCCCG
CAGGTGTTCACGGAGTTCAGCAGGCAGCGGGGCCTCGCGGCGGACGGCCGCTGCAAGGCC
TTCGCCGCGGCGGCCGACGGCACCGGTTTCTCCGAGGGCGTGGGCGTGCTGCTGGTGGAG
CGCCTGTCCGACGCCCGCCGCAACGGCCACCAGGTGCTCGCCGTCGTCCGCGGCTCCGCC
GTCAACCAGGACGGCGCCAGCAACGGCCTCACCGCACCCAACGGCCCCTCCCAGCAACGG
GTGATCCGCCAGGCCCTCGCCAACGCCCGCCTCACCCCCGCCGACGTCGACGCCGTCGAG
GCGCACGGCACCGGCACCACGCTCGGCGACCCCATCGAAGCCCAAGCCCTCCTCGCCACC
TACGGGCAGAACCGCACGGAGGACCAGCCGCTGTGGCTCGGCTCGGTGAAGTCCAACATC
GGCCACACGCAGGCCGCCGCCGGTGTCGCGGGCGTCATCAAGATGGTCATGGCGCTGCGC
CACGAGGTACTGCCACAGACACTGCACGTCGACGCGCCCTCACCCCACATCGACTGGGCG
TCGGGCCACGTCTCCCTCCTCACCGAGGAACTGAAGTGGCCGGGGACGGACCGGCCGCGC
CGGGCCGGTGTGTCCTCCTTCGGCATCAGCGGCACCAACGCCCACGTCATCCTTGAAGCC
GCCCCGCCGGAGACCCCGCCCGCCGATGACGAGCCGGAGCGGGCGGCCGGACCGGTGCCG
TGGGTCCTCTCCGCCAGGACCAGGAAGGCCCTCCAGTCCCAGGCGGCCCGGTTGCTCACC
TTCACCGAGGAGACCCCGGACGTCTCCGTCGCCGCCGTGTCCTCCGCGCTGCTCGGCACG
CGTGCCGCCCTCGACCACCGGGCCGTCGTCCTGGCCTCGGACCGTGTCGAATTCTCACGA
CGCCTGCACGCACTGGCGTCCGGCAGGCCCGCGAGCGGAGTGACCACCGGGAGCGTGCGG
TCGGGGCGGCTGGCGTTCATGTTCACCGGCCAGGGCTCCCAACGCCTCGGCATGGGCCAC
GAGCTCTACGACACCGAACCCGCCTTCGCCGCAGCACTGGAAGAAGCCTTCGCAGCACTG
GACCCACACCTGCCACAGCCACTGCGCGACATCATCTTCGGCGACGACCCCGAGCTACTG
CAGCAAACCCAGTACGCACAACCCGCGCTGTTCGCCGTCGAGACCGCCCTGTACCGGCTC
CTCACCGACACCTACGGCCTGCGCCCCGACTACCTCCTCGGCCACTCCATCGGAGAACTC
ACCGCCGCCCACGCCGCCGGCGTCCTCACCCTGCCCGACGCCGCACGCCTGGTCACCGCC
CGCGGACACCTCATGCAAACCGCACCCACCGGCGGAACCATGATCGCCATCCAAGCCACG
GAAGACGAAATCACCGAACACCTGACCCCCGGGGTGGCTATCGCCGCCCTCAACTCCCCC
AGCAGCACAGTCATATCGGGTGACACCGCCGAGGCAGAAGCCATCGCGGACCGCTTCCGC
GCCCTCGGCCGCAAGACCAGCCGACTGAAGGTCTCCCACGCCTTCCACTCCCCCCACATG
GACCCCGTCCTCGACGACTTCCGGCAGATCGCCGCCACCCTCGACTACCACCCCGCCCGC
ATCCCCGTCATCTCCAACACCACCGGACAGCCCGCCGAAAACCTCACATCCCCCGACTAC
TGGACCCAACACATCCGCCGCCCCGTCCGCTTCACCGACAGCCTCCACCACCTCACCCAG
CAGGGTGTGACGACCTTCCTCGAACTCGGTCCCGATGCCACCCTCGCCATGCTCGCCGAC
GCCCACGCCACCGCCGTCCTGCGCAGGAACTCGTCCGAGCCGCAGGCCCTGCGTACGGCG
CTCGCCGAGCTGTGGGTCCACGGCGTTCCGGTGGCTTGGCCTCACTCGACCGACCGGCGC
GTGGATCTGCCGACCTACGCCTTCGACCACCAGCACTACTGGCTGGACGCGGCGCCCGGA
ACCTCCGCCACCTCCGTCCGCCCGTCGGTGCCGGTGGAGGCCGCCGACGCACCGCCCACG
CTCGCTGATCGGTTCGAGGGACTGGGCGCGGGCGAACGGACCTCCATGGCTGAGGAGTTG
GTCCGTGAGACGGTCGCTGTCGTCCTGGGGTTCGCCGACCCGGAGGACGTGGACGTGTCC
ACGCCGTTCATCAAGCTGGGCATCGACTCACTGACGGCGGTGGAACTGCGCAGGCGGCTG
GGCGGGTACACCGGCCTGGAGATCCCCGCCGTGGTCGTGTTCGAGCATCCGACCACCCAC
GACCTCGCCGAGTACCTGATCGAGGCGATGTCCGCGCCTCCGGGAGCGACGCCGGACGGC
GCTTCGGGTGCGCCTTCCGGCGTTCGGGCGACCGCCGCGGTGGACTCGCTCTCGGATCTC
TTCGTCCGGGCGTGTGGACGGGGGGAGTTCGCCGAGGCCCACCGTCTCGCGCTGGGGCTC
GCCGAGTACCGGCCCACCTTCGCGGGCGAGCTGGACGGGGCGCGGTCGCCGGACGTCGTA
CCGCTTCGTCGAGGTACGGCGTCGCCGGCCGTGGTGTGCTTCCCGTCGTTCGTCTGGAAG
CCCGACTTCCAGCAGTACCTGTCGCTGGCCCGGGAGTTGACCGAGGACCGGGACGTCGCG
GTGTCGGGGCTGCCCGGGTTCCGGCCCGGGGAGCCGTTGCCCGCTTCGGCGGAGGCGCTG
GCGCGGGCGCTGGCGGCCGTGGTGCGCCGGGTCGCCGGTGGGCGGCGGTGTGTTCTGCTC
GGTCACTCGTCGGGGGGCTATGTGGCGGCGGCGGTGGCGGCACAGCTGGAGGAGGCGGGC
GAGGCGCCCGCCGGGCTGGTGCTGGTCGACACGCCGTGGTGGGGTTCCGCGGGCGGGCTG
GGGTCGCAGGAGTGGCTTCCGGTGATCAACGAGATGCTGCTGGCGCGCGGTGCGGCTGCG
GGTGCGGAGGACAGCGACGCGGGTGACGCCTGGGTCACCGCGCGGGCCAAGTACTTCAGC
CTGGACTTCGCGATCGCGCCACGGGATGTGCGGACCCTGCTGCTGCGGCCGGCCGAACCG
CTGGCCGGTTCCGCCGCGGACGCCGGCTGGCGCGCCGAGTGGCGGCCAGGCACCACGGTC
GTCGAGGTGCCGGGGAACCACTTCTCGATGCTGGACAGCGCCCATGCCCAGGCATGCGCC
AGGGCGGTGGAGGGCTGGCTCGCCACTCTGGGATAG
[11] KS35..411
[11] AT566..868
[11] malonyl-CoA753..757
[11] ACP937..1007
[11] TE1105..1307
[11] KS103..1233
[11] AT1696..2604
[11] malonyl-CoA2257..2271
[11] ACP2809..3021
[11] TE3313..3921

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1105-1307] 9.8e-26 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[753-859] 2.7e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   no description
IPR006162 Phosphopantetheine attachment site (PTM)
[965-980] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[940-1006] 7.8e-15 PF00550
PF00550   PP-binding
[937-1007] 21.804 PS50075
PS50075   ACP_DOMAIN
[930-1046] 1.6e-21 SSF47336
SSF47336   ACP-like
[937-1009] 3.5e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[35-285] 3.8e-100 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-411] 3.3e-51 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[566-868] 3.9e-64 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[563-861] 1.2e-72 SSF52151
SSF52151   FabD/lysophospholipase-like
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[690-752] 3.9e-16 SSF55048
SSF55048   Probable ACP-binding domain of malonyl-CoA ACP transacylase
IPR016038 Thiolase-like, subgroup (Domain)
[37-297] 9.4e-91 G3DSA:3.40.47.10 [298-464] 4.4e-67 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
[27-409] 9.7e-105 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[567-851] 5.2e-116 SM00827
SM00827   Acyl transferase domain in polyketide syntha
IPR020802 Polyketide synthase, thioesterase domain (Domain)
[1092-1307] 2.2e-54 SM00824
SM00824   Thioesterase
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[938-1010] 7.9e-25 SM00823
SM00823   Phosphopantetheine attachment site
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[37-463] 1.5e-233 SM00825
SM00825   Beta-ketoacyl synthase
SignalP No significant hit
TMHMM No significant hit
Lasal_00180 Lasal_00180   Lasal_00200 Lasal_00200
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