Lasal_00150 : CDS information

Location

Organism	Streptomyces lasaliensis
Strain	ATCC 35851
Entry name	Lasalocid
Contig	AB449340
Start / Stop / Direction	46,297 / 49,638 / + [in whole cluster] 46,297 / 49,638 / + [in contig]
Location	46297..49638 [in whole cluster] 46297..49638 [in contig]
Type	CDS
Length	3,342 bp (1,113 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	putative polyketide synthase
Gene
Gene (GenBank)	lsd13
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B6ZK66 PmId [19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009) comment GenBank,Uniprotでは、ATCC31180と登録されている。 S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。 lsd13: polyketide biosynthesis, module 6 module6(KS, AT, ACP)
Related Reference	ACC B5M9L0 NITE Lasal2_00180 PmId [19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008) comment GenBank,Uniprotでは、NRRL3382Rと登録されている。 S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。 lasAIII: polyketide synthase KS, AT, ACP module6(KS, AT, ACP)

PKS/NRPS Module

methylmalonyl-CoA

KS	40..416

AT	568..887

ACP	959..1029

Sequence

>polyketide synthase [putative polyketide synthase]
MEVSRMPNTEEKLLDYLKRVTADLKQTQRRLREAEAADTEPIAIVGMGCRYPGDVRSADD
LWGLLAEGGDAISKFPDDRGWNTEELYDPDPDRPGRNYVREGGFLHDATEFDADFFGISP
REALAMDPQQRIMLEVAWETFEHAGLDPQSFGGSDTGVFVGISNVDYTWGHARVPEAVEG
YFGTGNFASVLSGRLAYTFGLEGPALTVDTACSSSLVALHLATQALRRGECGAALVGGVT
VMSNPSGFVEFSRQRGLAPDGRCKAFAAAADGFGPSEGAGVLLVERLSDARRNGHRVLAV
VRGSAVNQDGASNGLSAPNGPSQQRVIRQALRNARLSPEQVDAVEAHGTGTTLGDPIEAQ
ALLATYGQGRPEDRPLWLGSVKSNIGHTSAAAGVAGVIKMVMAMRHGVLPRSLHIDEPTP
QVDWSSGAVTLLTEPVDWPDSDRPRRAGVSAFGISGTNAHVILEQAPTQDPQQPAPPVPA
APWLLSAKTPAALRAQARRLHTHLTRHPHPDPTDIAHALATTRTPHEHRAALVTDDHGTL
LHGLLALGEESVAPYVVEPGRAVAGKTVFVFPGQGSQWTGMGRELLDTSEQFAAYIAECE
TALNDFVDWSLTDVLRGTEGAPGYDRVDVVQPALFAVMVSLARLWQHHGIHPDAVIGHSQ
GEIAAAHIAGALTLNDAARIVALRSQALLPLAGLGGMTSLALPHHQAHELIQPWGQDLSI
ASVNGPHSTVVSGTTHALDELHTTCNTQGVRARRIPVDYASHSAQVESIRDTVLQAATGI
NPQPTTIPLYSTVTGQPIDGTQLDADYWYTNLRHTVRFEETTRALLGSGHRHFIETTAHP
VLALALEETIEATGSDARVTGTLRRDHGDLTQLHTALATAWTHGVDVDWTAVVPGAGRRR
VDLPTYAFQRRRYWLEPGVVPVSDTVPVAVVDEAESGEAATALARALADASEAGREALLI
DLVQSRVAAVLGHEEPESVDTERAFKDLGFDSLTATQLRNRLNAATGLRLPATLVFSHPT
PKALAQYLAGVIRPDEEAAARPPALRDIDLLEDSLWAQSFDAATRDLVARRLDALLRRWS
AGGTTEEGVDEAALDTASDDELFQILDEQLGNA

>polyketide synthase [putative polyketide synthase]
ATGGAGGTGTCACGGATGCCGAACACGGAAGAGAAACTCCTCGACTACCTGAAGCGGGTC
ACCGCCGACCTCAAGCAGACGCAGCGACGCCTGCGTGAGGCCGAGGCCGCGGACACGGAG
CCGATCGCGATCGTCGGCATGGGCTGCCGCTACCCGGGCGACGTACGGTCCGCCGACGAC
CTGTGGGGGCTGCTGGCCGAGGGCGGCGACGCGATCTCGAAGTTCCCCGACGACCGAGGC
TGGAACACCGAGGAGCTCTACGACCCCGACCCCGACCGCCCCGGCCGCAACTACGTGCGA
GAGGGCGGCTTCCTGCACGACGCGACGGAGTTCGACGCCGACTTCTTCGGCATCTCCCCG
CGTGAGGCACTCGCCATGGATCCGCAGCAGCGCATCATGCTGGAGGTCGCCTGGGAGACG
TTCGAGCACGCGGGCCTGGACCCCCAGTCGTTCGGCGGCAGCGACACCGGCGTGTTCGTC
GGCATCAGCAACGTCGACTACACGTGGGGTCACGCCCGCGTCCCCGAGGCCGTCGAGGGG
TACTTCGGCACGGGCAACTTCGCCAGCGTGCTGTCCGGCCGGCTGGCCTACACGTTCGGG
CTGGAAGGCCCTGCGCTCACCGTCGACACGGCCTGCTCATCGTCGCTCGTGGCCCTGCAC
CTGGCGACGCAGGCCCTGCGCCGGGGCGAGTGCGGGGCAGCCCTGGTGGGCGGGGTCACC
GTGATGTCGAACCCGTCCGGCTTCGTGGAGTTCAGCCGCCAGCGGGGGCTCGCTCCGGAC
GGCCGCTGCAAGGCGTTCGCCGCGGCCGCCGACGGGTTCGGGCCGTCCGAGGGCGCGGGC
GTGCTGCTCGTGGAGCGCCTGTCCGACGCCCGCCGCAACGGTCACCGGGTACTGGCCGTC
GTCCGCGGCTCCGCCGTCAACCAGGACGGCGCCAGCAACGGCCTCTCCGCCCCCAACGGC
CCCTCCCAGCAGCGCGTGATCCGCCAGGCGCTCCGCAACGCACGGCTGTCACCGGAACAG
GTCGACGCCGTCGAGGCGCACGGCACGGGCACCACCCTGGGTGACCCGATCGAGGCGCAG
GCCCTGCTGGCCACCTACGGTCAGGGGCGTCCCGAGGACCGTCCGCTGTGGCTGGGCTCG
GTGAAGTCGAACATCGGGCACACGTCGGCCGCCGCCGGTGTGGCCGGGGTGATCAAGATG
GTGATGGCGATGCGGCACGGCGTCCTGCCCCGCAGCCTCCACATCGACGAGCCGACCCCG
CAGGTCGACTGGTCCTCCGGCGCCGTCACCCTGCTCACCGAACCCGTCGACTGGCCCGAC
TCCGACCGCCCCCGCCGCGCAGGCGTCTCCGCCTTCGGCATCAGCGGCACCAACGCCCAC
GTCATCCTCGAACAAGCCCCCACCCAAGACCCCCAGCAACCCGCCCCACCCGTACCCGCC
GCACCCTGGCTGCTCTCCGCCAAGACCCCGGCAGCACTGCGAGCCCAGGCCCGACGCCTG
CACACCCACCTCACCCGCCACCCCCACCCCGACCCCACCGACATCGCCCACGCCCTCGCC
ACCACCCGCACCCCCCACGAACACCGCGCCGCACTCGTCACCGACGACCACGGGACTTTG
TTACACGGACTGCTCGCGCTCGGGGAGGAGTCGGTAGCACCGTACGTGGTGGAGCCGGGC
AGAGCGGTAGCGGGAAAGACGGTGTTCGTGTTCCCGGGTCAGGGTTCGCAGTGGACCGGC
ATGGGACGCGAACTCCTCGACACCTCGGAGCAGTTCGCCGCCTACATCGCCGAGTGCGAG
ACCGCGCTCAACGACTTCGTGGACTGGTCGCTCACCGACGTCCTGCGCGGCACCGAAGGA
GCCCCCGGCTACGACCGCGTCGACGTCGTCCAACCCGCCCTCTTCGCCGTCATGGTCAGC
CTCGCCCGCCTCTGGCAACACCACGGCATCCACCCCGACGCCGTCATCGGCCACTCCCAA
GGCGAAATCGCCGCCGCCCACATCGCCGGAGCCCTCACCCTCAACGACGCCGCCCGCATC
GTCGCCCTACGCTCCCAAGCCCTCCTCCCCCTCGCCGGCCTCGGCGGCATGACCTCCCTC
GCCCTCCCCCACCACCAAGCCCACGAACTCATCCAGCCCTGGGGCCAAGACCTGTCCATC
GCCTCCGTCAACGGACCCCACTCCACCGTCGTCTCCGGCACCACCCACGCCCTCGACGAA
CTACACACCACCTGCAACACCCAAGGAGTTCGGGCCCGCCGCATCCCCGTCGACTACGCC
TCCCACTCCGCCCAAGTCGAAAGCATCCGCGACACCGTCCTCCAAGCAGCCACCGGCATC
AACCCCCAGCCCACCACCATCCCCCTCTACTCCACCGTCACCGGCCAACCCATCGACGGC
ACCCAGCTCGACGCCGACTACTGGTACACCAACCTCCGCCACACCGTCCGCTTCGAAGAA
ACCACCCGCGCTCTGCTCGGCTCCGGCCACCGCCACTTCATCGAAACCACCGCCCACCCC
GTCCTCGCCCTCGCCCTGGAAGAAACCATCGAGGCCACCGGCTCCGACGCCCGCGTCACC
GGCACCCTCCGCCGCGACCACGGCGACCTCACCCAACTCCACACCGCCCTCGCCACCGCC
TGGACCCACGGCGTCGACGTCGACTGGACCGCAGTGGTGCCCGGTGCGGGCAGGAGGCGG
GTGGACCTGCCGACGTACGCCTTCCAGCGTCGGCGTTACTGGCTCGAGCCGGGTGTCGTG
CCGGTGAGCGACACCGTCCCGGTGGCCGTGGTGGACGAAGCGGAGAGCGGGGAGGCGGCC
ACCGCGCTCGCACGCGCCCTGGCGGATGCCTCGGAGGCGGGCCGGGAGGCTCTGCTGATC
GACCTCGTACAGTCTCGCGTGGCCGCCGTGCTCGGTCACGAGGAGCCGGAGTCGGTCGAC
ACCGAGCGGGCCTTCAAGGACCTGGGCTTCGACTCGCTCACCGCGACGCAGTTGCGCAAC
CGGCTGAACGCGGCCACGGGGCTGCGCCTGCCGGCCACGCTCGTCTTCAGCCATCCGACT
CCCAAGGCGCTGGCCCAGTACCTGGCCGGCGTCATACGGCCCGACGAGGAGGCCGCCGCC
CGGCCGCCCGCCCTGCGGGACATCGATCTGCTGGAGGACAGCCTGTGGGCGCAGTCCTTC
GACGCCGCGACCCGTGACCTCGTCGCCCGGCGGCTCGACGCGCTCCTGCGCCGGTGGAGT
GCGGGCGGGACCACCGAGGAGGGCGTCGACGAGGCCGCCCTCGACACCGCCTCCGACGAC
GAGCTCTTCCAGATCCTCGACGAACAGCTGGGCAACGCCTGA

[6] KS	40..416

[6] AT	568..887

[6] methylmalonyl-CoA	759..763

[6] ACP	959..1029

[6] KS	118..1248

[6] AT	1702..2661

[6] methylmalonyl-CoA	2275..2289

[6] ACP	2875..3087

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) PF00550 PP-binding PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 ACP_like SSF47336 ACP_like IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking SSF101173 Polyketide_synth_docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS
SignalP	No significant hit
TMHMM	No significant hit

Lasal_00140 Lasal_00160