Medem_00160 Medem_00160   Medem_00180 Medem_00180

Medem_00170 : CDS information

close this sectionLocation

Organism
StrainAM-7161
Entry nameMedermycin
Contig
Start / Stop / Direction16,063 / 17,127 / + [in whole cluster]
16,063 / 17,127 / + [in contig]
Location16063..17127 [in whole cluster]
16063..17127 [in contig]
TypeCDS
Length1,065 bp (354 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glucose-1-phosphate thymidylyltransferase
putative dTDP-glucose synthase
Product (GenBank)NDP-1-glucose synthase
Gene
Gene (GenBank)med-ORF18
EC number2.7.7.24
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003)
comment
entire medermycin(med) biosynthetic gene clusterの報告。
Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。

ORF18: NDP-1-glucose synthase

配列解析のみ。
gra-ORF16 homolog.
Related Reference
ACC
Q2HR55
NITE
Sch475_00270
PmId
[18051285] Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. (J Microbiol Biotechnol. , 2007)
comment
Blast 4th, id65%, 1e-129
Streptomyces sp. SCC 2136_schS6
Putative dNDP-glucose synthetase

schS6をE.coliで発現、精製、酵素活性測定。
glucose-1-phosphate thymidylyltransferase活性を示す。
dTTP + alpha-D-glucose-1-phosphate → dTDP-D-glucose + pyrophosphate

close this sectionSequence

selected fasta
>putative glucose-1-phosphate thymidylyltransferase [NDP-1-glucose synthase]
MKALVLSGGAGTRLPPFSYSMPKQLIPIANKPVLEHVLENIRAVGITEVGVIVGDWADEI
AQVIGDGSRLGLRITLIPQEKPLGLAHCVALARPFLGDDDFVMYLGDNMLPGGIDTIAAD
FTARRPDALVVVHKVRDPPAFGVAELAPDGSVLRLVEKPERPRSDLALIGVYFFTAAIHE
AVAAIEPGARGELEITDAVQWLVAHGSHVAASEYDGYWKDTGRVEEVLACNRELLGDQTA
RVDGEVDAASRVTGRVVLEAGARLVRSTVTGPAVIGAGTVIEDSAVGPHTSIGRGCLLRD
TTLTDSIVMDGASITAVTGLQGSLIGRNAVVGAGAEGDGHRLVIGDHTRIEVAA
selected fasta
>putative glucose-1-phosphate thymidylyltransferase [NDP-1-glucose synthase]
ATGAAGGCCCTCGTGCTGTCCGGCGGAGCGGGCACCCGCCTGCCCCCCTTCAGCTACTCG
ATGCCCAAGCAGCTCATCCCCATCGCCAACAAGCCCGTCCTCGAACACGTCCTGGAGAAC
ATCCGCGCGGTGGGCATCACCGAGGTCGGCGTCATCGTCGGCGACTGGGCCGACGAGATC
GCCCAGGTGATCGGCGACGGCTCCCGGCTGGGGCTGCGCATCACCCTGATCCCCCAGGAG
AAGCCGCTCGGCCTCGCGCACTGCGTCGCCCTCGCCCGGCCCTTCCTCGGCGACGACGAC
TTCGTGATGTACCTCGGCGACAACATGCTCCCGGGCGGGATCGACACGATCGCCGCCGAC
TTCACCGCCCGACGGCCCGACGCCCTGGTCGTCGTCCACAAGGTGCGCGACCCCCCGGCC
TTCGGCGTGGCCGAACTCGCCCCCGACGGGAGCGTGCTGCGGCTCGTGGAGAAGCCCGAG
CGGCCCAGGAGCGACCTGGCCCTCATCGGCGTGTACTTCTTCACCGCCGCCATCCACGAG
GCCGTCGCCGCCATCGAGCCCGGCGCCCGCGGCGAACTGGAGATCACCGACGCCGTCCAG
TGGCTCGTCGCCCACGGCTCGCACGTCGCGGCCAGCGAGTACGACGGGTACTGGAAGGAC
ACCGGCCGGGTCGAGGAGGTCCTCGCCTGCAACCGGGAGCTGCTCGGCGACCAGACGGCC
CGGGTGGACGGCGAGGTCGACGCGGCCAGCCGGGTGACCGGCCGCGTCGTGCTGGAGGCG
GGAGCCCGCCTCGTGCGTTCCACGGTCACCGGACCGGCCGTCATCGGCGCCGGCACGGTG
ATCGAGGACAGCGCGGTCGGACCCCACACCTCCATCGGCCGGGGCTGTCTCCTGCGCGAC
ACGACCCTGACCGACTCCATCGTCATGGACGGGGCCTCCATCACCGCCGTGACCGGCCTG
CAGGGCTCGCTGATCGGCCGCAACGCCGTCGTCGGCGCCGGCGCCGAGGGCGACGGGCAC
CGTCTCGTGATCGGCGACCACACCCGTATCGAGGTGGCGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-234] 6.40000000000004e-50 PF00483
PF00483   NTP_transferase
IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family)
[2-351] 1.69999999999998e-122 TIGR01208
TIGR01208   RmlA_long
IPR011004 Trimeric LpxA-like (Domain)
[267-351] 3.00000067992871e-06 SSF51161
SSF51161   Trimer_LpxA_like
SignalP
[1-20] 0.226 Signal
Eukaryota   
TMHMM No significant hit
Medem_00160 Medem_00160   Medem_00180 Medem_00180
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