Medem_00150 Medem_00150   Medem_00170 Medem_00170

Medem_00160 : CDS information

close this sectionLocation

Organism
StrainAM-7161
Entry nameMedermycin
Contig
Start / Stop / Direction14,936 / 16,066 / + [in whole cluster]
14,936 / 16,066 / + [in contig]
Location14936..16066 [in whole cluster]
14936..16066 [in contig]
TypeCDS
Length1,131 bp (376 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 aminotransferase
Product (GenBank)NDP-deoxyhexose 3-aminotransferase
Gene
Gene (GenBank)med-ORF20
EC number2.6.1.-
Keyword
  • angolosamine
Note
Note (GenBank)
Reference
ACC
PmId
[12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003)
comment
entire medermycin(med) biosynthetic gene clusterの報告。
Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。

ORF20: NDP-deoxyhexose 3-aminotransferase

配列解析のみ。dnrJ homolog.
medermycinのdeoxysugarであるangolosamine生合成経路で、
NDP-3,4-diketo-2,6-dideoxyglucose → NDP-3-amino-4-keto-2,3,6-trideoxyglucose
へのC-3 aminotransferが割り当てられている。
Related Reference
ACC
Q2PC67
NITE
Rubra_00230
PmId
[16614891] Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. (Biotechnol Lett. , 2006)
comment
Blast 10th, id76%, 1e-164
Streptomyces achromogenes subsp. rubradiris_rubN4
Putative aminotransferase
[Rubra_00230]NDP-hexose 3-aminotransferase

abstract:
rubN6 and rubN4をHis-tagged fusion proteinsとしてE.coliで異種性に発現。
それぞれのTDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferaseの機能性を、in vitro enzyme assayによって検証。
ACC
Q9F837
NITE
Megalo_00050
PmId
[16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006)
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
Blast 15th, id74%, 1e-159
Micromonospora megalomicea subsp. nigra_megDII
TDP-3-keto-6-deoxyhexose 3-aminotransaminase
[DoBISCUIT]NDP-hexose C-3 aminotransferase

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。セットの組み合わせによりMegDII の機能は確立されている。

MegDII は、desosamineとmegosamine両方の生合成において3-aminotransferaseとして働く。
基質は各経路で少し異なる。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 aminotransferase [NDP-deoxyhexose 3-aminotransferase]
MTTYVWDYLPEYENERDDLIDAVDTVFRSGRLILGPSVEGFESEFAAYHGLAHCAGVDNG
TNAVKLGLQALGVGPGDEVITVSNTAAPTVVAIDGTGATPVFVDIREQDFLMDTDQGAAA
ITPRTKVLLPVHLYGQCVDMAPLERLAAQHGLKILEDCAQSHGAHHHGRTAGTIGDAAAF
SFYPTKVLGAYGDAGAVVTDDPEVDAALRRLRYYGMAETYYVVQTPGHNSRLDEVQAEIL
RRKLRRLDTYIEGRRAVARRYEEGLGDLVAPDGLRLPVTAEGNDHVHYVYVVRHPRRDEI
IERLKGYGISLNISYPWPVHTMSGFAHLGYAPGSLPVTERLAGEIFSLPMYPSLSEDVQD
KVISALREVLTEGTRS
selected fasta
>putative NDP-hexose C-3 aminotransferase [NDP-deoxyhexose 3-aminotransferase]
ATGACCACCTACGTCTGGGACTACCTCCCGGAGTACGAGAACGAACGCGACGACCTCATC
GACGCGGTGGACACCGTCTTCCGCTCCGGCCGCCTCATCCTCGGCCCGTCGGTCGAGGGC
TTCGAGAGCGAGTTCGCCGCCTACCACGGCCTCGCGCACTGCGCCGGCGTGGACAACGGC
ACCAACGCCGTGAAGCTCGGCCTCCAGGCCCTCGGCGTCGGCCCCGGCGACGAGGTCATC
ACCGTCTCCAACACCGCCGCGCCCACCGTCGTCGCCATCGACGGCACCGGAGCCACCCCC
GTGTTCGTGGACATCCGCGAACAGGACTTCCTGATGGACACCGACCAGGGGGCGGCGGCC
ATCACCCCCCGGACCAAGGTCCTGCTGCCCGTCCACCTCTACGGCCAGTGCGTGGACATG
GCCCCGCTGGAGCGCCTCGCCGCACAGCACGGCCTGAAGATCCTCGAGGACTGCGCCCAG
TCGCACGGCGCCCACCACCACGGCCGGACGGCGGGCACCATCGGCGACGCCGCGGCCTTC
TCCTTCTACCCGACCAAGGTCCTCGGCGCGTACGGCGACGCCGGCGCGGTCGTCACCGAC
GACCCGGAGGTCGACGCCGCCCTGCGGCGCCTGCGCTACTACGGCATGGCGGAGACCTAC
TACGTCGTCCAGACCCCCGGACACAACTCCCGCCTCGACGAGGTGCAGGCCGAGATCCTC
CGGCGCAAGCTCCGCCGCCTCGACACCTACATCGAGGGCCGCCGGGCGGTCGCCCGCCGC
TACGAGGAGGGGCTCGGGGACCTCGTCGCGCCGGACGGGCTCCGGCTGCCCGTCACCGCC
GAGGGCAACGACCACGTCCACTACGTCTACGTCGTCCGCCACCCCCGGCGCGACGAGATC
ATCGAGCGGCTCAAGGGCTACGGCATCTCGCTGAACATCAGCTACCCCTGGCCGGTGCAC
ACGATGAGCGGCTTCGCACACCTCGGATACGCCCCGGGCTCGCTGCCCGTGACCGAGCGG
CTGGCCGGCGAGATCTTCTCCCTGCCGATGTACCCCTCGCTCTCCGAGGACGTCCAGGAC
AAGGTGATCTCCGCCCTGCGGGAGGTCCTCACCGAGGGGACCCGGTCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-374] 1.39999277195148e-102 PIRSF000390
PIRSF000390   PLP_StrS
[15-367] 4.30000000000003e-116 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[16-246] 9.00000000000011e-77 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[247-371] 4.10000000000004e-38 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[6-374] 3.30001976117415e-106 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Medem_00150 Medem_00150   Medem_00170 Medem_00170
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