Medem_00180 Medem_00180   Medem_00200 Medem_00200

Medem_00190 : CDS information

close this sectionLocation

Organism
StrainAM-7161
Entry nameMedermycin
Contig
Start / Stop / Direction18,122 / 19,561 / + [in whole cluster]
18,122 / 19,561 / + [in contig]
Location18122..19561 [in whole cluster]
18122..19561 [in contig]
TypeCDS
Length1,440 bp (479 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose 2,3-dehydratase
Product (GenBank)NDP-deoxyglucose-2,3-dehydratase
Gene
Gene (GenBank)med-ORF16
EC number
Keyword
  • angolosamine
Note
Note (GenBank)
  • GTG start codon
Reference
ACC
PmId
[12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003)
comment
entire medermycin(med) biosynthetic gene clusterの報告。
Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。

ORF16: NDP-deoxyglucose-2,3-dehydratase

配列解析のみ。gra-orf27 homolog.
deoxysugar生合成での共通ステップ
glucose-1-P → EC 2.7.7.24 → EC 4.2.1.46 → dTDP-4-keto-6-deoxyglucose
に続く2,3-dehydrationが、angolosamine生合成の提唱経路で割り当てられている。
Related Reference
ACC
Q845B8
PmId
[12889805] Functional identification of rub52 gene involved in the biosynthesis of rubradirin. (Biotechnol Lett. , 2003)
comment
Blast 13th, id62%, 1e-147
Streptomyces achromogenes_rub52
DTDP-glucose-2,3-dehydratase

Streptomyces achromogenes var. nibradiris NRRL3061のrub52を、E.coliで可溶性His-tagged fusion proteinとして発現。in vitro enzyme assayからthymidine diphospho-4-keto-6-deoxyglucoseのC-2 deoxygenation機能性が割り当てられた。
ACC
Q9ALN6
NITE
Spino_00070
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 40th, id51%, 1e-119
Saccharopolyspora spinosa_spnO
Probable NDP-hexose-2,3-dehydratase
[Spino_00070]dTDP-4-keto-6-deoxyglucose 2,3-dehydratase

Forosamine合成の初期段階で
NDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。

dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。
dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。

--
SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

close this sectionSequence

selected fasta
>putative NDP-hexose 2,3-dehydratase [NDP-deoxyglucose-2,3-dehydratase]
MTGTPDTATRAALPGPDPATAALRRRAETGLAERLARSAAATEGAGPRTDEVADWLADPV
RAHRFRVDRIPFAALDGWSFAPGSGNLVHRSGRFFSVEGVGVTVDHGPFPQWQQPIIHQP
EVGILGILAKEIDGVLHFLMQAKMEPGNRNLLQLSPTVQATRSNYTKVHKGADVRYLEYF
LGHGRGRVLADVLQSEHGSWFLRKANRNVLVETDEDVPAHEDFRWLTLGQIGRLLRLDNL
VNMDARTVLAGLPTSSADDRLARALHTDRELLSWITAQRARHEVRVERLPLAEVRGWHRT
ATAVERADGRYFRVVAVAVEAGSRETADWTQPLIEPTGRGVTAFLNPRFGGVPHLLVHAR
GEAGLLDVLELAPTVQYVPENYAHLDPDDRPPFLAEVLAADPRRIRYEAVHSEEGGRFLD
AESRYLVVEADPGTPPDPPEGYLWVTPGQLGALAQHARYVNVQARTLLACINAGAVGPL
selected fasta
>putative NDP-hexose 2,3-dehydratase [NDP-deoxyglucose-2,3-dehydratase]
GTGACCGGCACCCCGGACACCGCCACCCGGGCGGCGCTGCCCGGGCCGGACCCCGCGACG
GCGGCACTGCGCCGCCGCGCGGAGACCGGCCTGGCCGAGCGGCTCGCCCGCTCGGCCGCC
GCGACGGAGGGAGCCGGACCCCGCACGGACGAGGTCGCCGACTGGCTGGCGGACCCCGTG
CGGGCCCACCGGTTCCGGGTCGACCGCATCCCCTTCGCCGCCCTCGACGGCTGGTCGTTC
GCACCGGGCTCCGGGAACCTCGTCCACCGCAGCGGCCGGTTCTTCTCGGTCGAGGGCGTC
GGCGTCACCGTCGACCACGGGCCCTTCCCGCAATGGCAGCAGCCCATCATCCACCAGCCC
GAGGTCGGCATCCTCGGCATCCTGGCCAAGGAGATCGACGGAGTCCTGCACTTCCTGATG
CAGGCGAAGATGGAGCCGGGCAACCGGAACCTGCTCCAGCTCTCCCCGACGGTCCAGGCC
ACCCGCAGCAACTACACCAAGGTGCACAAGGGCGCGGACGTGCGCTACCTGGAGTACTTC
CTCGGGCACGGTCGCGGGCGGGTGCTCGCCGACGTCCTCCAGTCCGAGCACGGCTCCTGG
TTCCTGCGCAAGGCCAACCGGAACGTCCTCGTGGAGACCGACGAGGACGTGCCGGCGCAC
GAGGACTTCCGCTGGCTGACCCTCGGTCAGATCGGACGGCTCCTCCGGCTCGACAACCTC
GTCAACATGGACGCGCGCACCGTCCTCGCCGGGCTGCCGACCTCCTCCGCCGACGACCGC
CTCGCCCGGGCGCTGCACACCGACCGCGAACTGCTCTCCTGGATCACCGCCCAACGCGCC
CGCCACGAGGTCCGCGTGGAGCGGCTCCCGCTCGCCGAGGTGCGCGGCTGGCACCGTACC
GCCACCGCCGTCGAGCGCGCGGACGGCCGCTACTTCCGCGTGGTGGCCGTGGCCGTCGAG
GCCGGCAGCCGCGAGACCGCCGACTGGACGCAGCCGCTCATCGAACCGACCGGGCGGGGC
GTGACCGCCTTCCTCAACCCCCGCTTCGGCGGCGTCCCCCACCTCCTGGTGCACGCGCGC
GGGGAGGCCGGCCTGCTCGACGTCCTGGAGCTGGCCCCCACCGTGCAGTACGTCCCGGAG
AACTACGCCCACCTCGACCCCGACGACCGGCCGCCGTTCCTGGCGGAGGTCCTCGCGGCC
GACCCCCGGCGCATCCGCTACGAGGCGGTCCACTCCGAGGAGGGCGGCCGGTTCCTCGAC
GCCGAGAGCCGCTATCTCGTCGTCGAGGCCGACCCCGGGACCCCGCCCGACCCGCCGGAG
GGCTACCTGTGGGTCACCCCCGGCCAGCTGGGAGCCCTCGCCCAGCACGCGCGCTATGTG
AACGTCCAGGCCCGCACGCTGCTCGCCTGCATCAACGCAGGTGCCGTCGGGCCGCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005212 NDP-hexose 2,3-dehydratase (Domain)
[49-252] 2.70000000000002e-86 PF03559 [266-471] 3.99999999999998e-79 PF03559
PF03559   Hexose_dehydrat
SignalP
[1-32] 0.189 Signal
Bacteria, Gram-positive   
[1-41] 0.122 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Medem_00180 Medem_00180   Medem_00200 Medem_00200
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