Medem_00200 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	19,612 / 20,334 / + [in whole cluster] 19,612 / 20,334 / + [in contig]
Location	19612..20334 [in whole cluster] 19612..20334 [in contig]
Type	CDS
Length	723 bp (240 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose N,N-dimethyltransferase
Product (GenBank)	N-methyl transferase
Gene
Gene (GenBank)	med-ORF15
EC number	2.1.1.-
Keyword	angolosamine
Note
Note (GenBank)
Reference	ACC Q7WT22 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF15: N-methyl transferase 配列解析のみ。snogX homolog. medermycinのdeoxysugarであるangolosamine生合成経路で、 NDP-3-amino-2,3,6-trideoxyglucose → NDP-angolosamine へのC-3 N,N-dimethylationが割り当てられている。
Related Reference	ACC Q9ZGH6 NITE Pikro_00100 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002) comment Blast 9th, id52%, 2e-59 Streptomyces venezuelae_desVI N,N-dimethyltransferase --- [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesVI: N,N-Dimethyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。 --- [PMID: 12119032](2002)abstract N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。 TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

Sequence

>putative NDP-hexose N,N-dimethyltransferase [N-methyl transferase]
MYGNDSAELYDLLHSARGKDFDGEAAAVAALVDDRMPGAASLLDIACGTGAHLARFAERY
ERVEGVELSEPMLALARTRLPGVPFTLGDMRDFDLGRRFDAAVCLFGSIGYVDSADELDA
TLRSFARHLNPGGVLVVEPWWAPDTYTPGHVSADVVEADGTHVARVSHASLATPAGDASL
MRRSYVVAHADHGVRHFSEDHVCRLFGADRYEAAFTAAGFAFERVASPVSARGFLVGVRK

>putative NDP-hexose N,N-dimethyltransferase [N-methyl transferase]
ATGTACGGAAACGACTCCGCCGAGCTCTACGACCTCCTGCACAGCGCCCGGGGCAAGGAC
TTCGACGGCGAGGCCGCCGCGGTCGCCGCGCTCGTCGACGACCGCATGCCCGGCGCCGCC
TCCCTCCTCGACATCGCCTGCGGCACCGGCGCCCACCTCGCCCGCTTCGCCGAGCGGTAC
GAGCGCGTCGAGGGCGTGGAACTCTCCGAGCCCATGCTGGCCCTCGCCCGGACCCGGCTG
CCCGGCGTCCCGTTCACCCTCGGCGACATGCGCGACTTCGACCTGGGCCGCCGCTTCGAC
GCCGCGGTCTGCCTGTTCGGCTCCATCGGCTACGTCGACTCGGCCGACGAACTCGACGCC
ACCCTGCGCTCCTTCGCCCGCCACCTCAACCCCGGCGGCGTCCTGGTCGTGGAACCGTGG
TGGGCCCCGGACACCTACACCCCCGGGCACGTCTCCGCCGACGTGGTCGAGGCCGACGGA
ACGCACGTGGCCCGCGTCTCGCACGCCTCGCTCGCCACGCCCGCCGGCGACGCCTCCCTC
ATGCGCCGCTCCTACGTGGTGGCCCACGCCGACCACGGCGTAAGGCACTTCAGCGAGGAC
CACGTGTGCCGGCTCTTCGGCGCCGACCGGTACGAGGCCGCCTTCACCGCCGCGGGCTTC
GCCTTCGAGCGGGTCGCGAGCCCGGTGTCCGCCCGCGGGTTCCTCGTCGGGGTCCGCAAG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota
TMHMM	No significant hit

Medem_00190 Medem_00210