Medem_00210 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	20,331 / 21,308 / + [in whole cluster] 20,331 / 21,308 / + [in contig]
Location	20331..21308 [in whole cluster] 20331..21308 [in contig]
Type	CDS
Length	978 bp (325 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	NDP-4-keto-6-deoxyhexose reductase
Gene
Gene (GenBank)	med-ORF14
EC number
Keyword	angolosamine
Note
Note (GenBank)	GTG start codon
Reference	ACC Q7WT21 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF14: NDP-4-keto-6-deoxyhexose reductase 配列解析のみ。gra-orf22 homolog. 本文中でmed-ORF'15'と間違って記載されている。 medermycinのdeoxysugarであるangolosamine生合成経路で、 NDP-3-amino-4-keto-2,3,6-trideoxyglucose → NDP-3-amino-2,3,6-trideoxyglucose への4-ketoreductionが割り当てられている。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment 6th(O33708) 43%, 1e-49 Streptomyces peucetius_dnmV thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [Adria_00200]NDP-hexose C-4 ketoreductase --- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 --- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [NDP-4-keto-6-deoxyhexose reductase]
MTAGPPTVPRTAPARRVMVLGGTGFVGRTVCADFRAAGWDVLAVARTAPERPAATRFLAL
DLAETPSATLARVLEAERPDVVVNATGSIWSRDDAAMERICTVPTLRLLDALATLRHRPR
LVHLGTVLEYGPLPPDGTPRPDPAPEPTTPYGRAKLAPSRAVLEAAASGAVRALVLRVSN
VSGVGAPDVSLLGRVAERLVTAPPAAGRSPSNSPRCGPTRHYVDVRDVSEAVLAAALSPA
SGRAVDIGRGEAVPVRWLVDLLVRVSGVPARIVERPAEPGAASERDWIRTDLAPAREVLG
WRPRRGLQESLAAYWADVSGRPAPV

>putative NDP-hexose C-4 ketoreductase [NDP-4-keto-6-deoxyhexose reductase]
GTGACGGCCGGACCGCCCACCGTCCCGCGGACCGCACCCGCCCGGCGCGTCATGGTGCTC
GGCGGCACGGGCTTCGTGGGGCGGACCGTCTGCGCGGACTTCCGCGCCGCCGGCTGGGAC
GTCCTGGCCGTGGCACGGACCGCGCCGGAGAGGCCCGCCGCGACCCGGTTCCTCGCCCTC
GACCTGGCGGAGACACCGTCGGCCACGCTCGCCCGCGTCCTGGAGGCCGAACGCCCCGAC
GTGGTCGTCAACGCCACCGGGAGCATCTGGAGCCGGGACGACGCCGCCATGGAACGCATC
TGCACCGTCCCCACCCTGCGGCTCCTCGACGCCCTCGCCACGCTGCGGCACCGGCCCCGT
CTCGTCCACCTCGGCACCGTGCTCGAGTACGGTCCGCTGCCGCCCGACGGCACCCCCCGC
CCCGACCCGGCACCCGAGCCGACCACCCCGTACGGAAGGGCGAAGCTCGCGCCGTCCCGC
GCCGTCCTGGAGGCGGCCGCGAGCGGCGCCGTGCGGGCCCTCGTCCTGCGCGTCTCGAAC
GTGTCGGGCGTCGGCGCCCCCGACGTCAGCCTGCTCGGCAGGGTCGCCGAGCGCCTGGTG
ACGGCTCCGCCGGCGGCGGGACGGTCACCGTCGAACTCGCCCCGCTGCGGGCCCACCCGG
CACTACGTCGACGTACGGGACGTGTCGGAGGCCGTGCTCGCGGCGGCGCTCTCGCCCGCC
AGCGGCCGGGCCGTCGACATCGGACGCGGCGAGGCCGTGCCCGTGCGGTGGCTCGTGGAC
CTCCTGGTGCGGGTGAGCGGGGTGCCCGCCCGGATCGTCGAACGGCCGGCGGAGCCCGGC
GCCGCCTCGGAGCGGGACTGGATCCGGACGGATCTCGCCCCGGCCCGTGAGGTCCTCGGC
TGGCGGCCGAGGCGCGGTCTGCAGGAGTCGCTCGCCGCCTACTGGGCGGACGTCAGCGGC
CGGCCCGCCCCCGTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-positive Eukaryota
TMHMM	No significant hit

Medem_00200 Medem_00220