Medem_00220 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	21,350 / 22,366 / + [in whole cluster] 21,350 / 22,366 / + [in contig]
Location	21350..22366 [in whole cluster] 21350..22366 [in contig]
Type	CDS
Length	1,017 bp (338 aa)

Annotation

Category	5.1 general function
Product	putative phosphotransferase
Product (GenBank)	putative carbohydrate kinase
Gene
Gene (GenBank)	med-ORF21
EC number
Keyword
Note
Note (GenBank)	GTG start codon
Reference	ACC Q7WT20 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF21: Putative carbohydrate kinase(possible kinase gene) 配列解析のみ。 regulatory genesのひとつとして述べられている。
Family/Domain	FD IPR011611, IPR002173 comment [IPR011611] Carbohydrate/purine kinase (Domain) no function registration [IPR002173] Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site) function; phosphotransferase activity, alcohol group as acceptor

Sequence

>putative phosphotransferase [putative carbohydrate kinase]
MRIAVTGSIATDHLLTFPGRFGEQLIEGELDKVSLSFLADELDVRRGGVAANIAFGLGAL
GLRPLLVGSVGRDFAEYGRLLAAHGVDTGSVHVSETRHTARFICTTDADQNQIGTFYAGA
MSEARHIALAPVRDRAGGLDLVLVSPNDPEAMLRHTREAAALGVAFSADPSQQLARLGRD
EVRLLLRGPRHLFTNAYEAQLVQERTGWTERQVLERVGTWVITRGADGVRILASGRPAVD
VAAVPADGPVEPTGAGDAFRAGFLAATARADDPVRAARLGAALATTALESVGPQAYTLDG
APLLARIADAYGPEAAADLEPEVKAVRPAETAVGAEPS

>putative phosphotransferase [putative carbohydrate kinase]
GTGCGTATCGCGGTGACAGGCTCCATCGCCACCGACCATTTGCTGACGTTTCCCGGCCGG
TTCGGGGAGCAGCTCATCGAGGGCGAACTGGACAAGGTCTCGCTCTCGTTCCTCGCCGAC
GAGCTCGACGTGCGCCGGGGCGGTGTGGCGGCGAACATCGCCTTCGGCCTCGGCGCCCTC
GGACTGAGGCCCCTCCTCGTCGGGTCCGTCGGCAGGGACTTCGCCGAGTACGGGCGGCTG
CTCGCGGCACACGGGGTCGACACCGGCTCCGTCCACGTCAGCGAGACCCGGCACACGGCC
CGGTTCATCTGCACCACCGACGCGGACCAGAACCAGATCGGCACCTTCTACGCGGGCGCC
ATGAGCGAGGCACGGCACATCGCCCTCGCACCGGTGCGCGACCGCGCCGGGGGCCTCGAC
CTGGTGCTCGTCTCGCCGAACGACCCGGAGGCGATGCTGCGGCACACCAGGGAGGCCGCC
GCGCTCGGCGTCGCGTTCAGCGCCGACCCCTCCCAGCAGCTGGCGCGGCTCGGCAGGGAC
GAGGTCCGGCTGCTCCTGCGCGGGCCCCGCCACCTCTTCACCAACGCGTACGAGGCCCAG
CTCGTCCAGGAACGGACGGGGTGGACCGAGCGGCAGGTCCTGGAGCGGGTCGGCACCTGG
GTGATCACCCGGGGCGCGGACGGTGTCCGGATCCTGGCGTCCGGCCGGCCCGCCGTCGAC
GTGGCGGCCGTCCCCGCGGACGGGCCCGTGGAGCCCACCGGAGCCGGCGACGCCTTCCGG
GCCGGATTCCTCGCGGCCACGGCCCGCGCGGATGATCCGGTGCGGGCCGCCCGCCTCGGC
GCCGCCCTCGCCACCACGGCCCTGGAGTCCGTGGGGCCGCAGGCGTACACGCTCGACGGC
GCGCCGCTCCTCGCCCGCATCGCGGACGCGTACGGGCCCGAGGCCGCCGCCGACCTGGAG
CCGGAGGTCAAGGCCGTACGCCCGGCCGAAACGGCGGTCGGCGCCGAGCCCTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002173 Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site) PS00583 PFKB_KINASES_1 IPR011611 PfkB (Domain) PF00294 PfkB
SignalP	No significant hit
TMHMM	No significant hit

Medem_00210 Medem_00230