Medem_00240 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	23,946 / 22,972 / - [in whole cluster] 23,946 / 22,972 / - [in contig]
Location	complement(22972..23946) [in whole cluster] complement(22972..23946) [in contig]
Type	CDS
Length	975 bp (324 aa)

Annotation

Category	3.3 modification reduction
Product	C-3 ketoreductase
Product (GenBank)	stereospecific keto reductase
Gene
Gene (GenBank)	med-ORF12
EC number
Keyword	S-stereochemistry
Note
Note (GenBank)
Reference	ACC Q7WT18 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) [16169737] Functional studies on a ketoreductase gene from Streptomyces sp. AM-7161 to control the stereochemistry in medermycin biosynthesis. (Bioorg Med Chem. , 2005) [22489461] [Expression detection of med-ORF12 encoding a stereochemical ketoreductase possibly involved in medermycin biosynthesis]. (Wei Sheng Wu Xue Bao. , 2012) comment [PMID:12855716](2003) entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF12: Stereospecific ketoreductase(Tailoring) 配列解析のみ。 actVI-ORF1 homologue -- [PMID:16169737](2005) abstract Medermycinはその強力な抗癌活性に重大なピラン環において、actinorhodinと同じtrans立体配置(3S, 15R)を示す。 actinorhodin生合成において、ActVI-ORF1がC-3での立体化学を決定すると既に証明されている。 actVI-ORF1不全のある株をmed-ORF12で機能的に相補できた。 actinorhodinの早期段階生合成遺伝子とmed-ORF12の同時発現から、med-ORF12がピラン環の形成においてC-3でエナンチオ選択性還元を担うketoreductaseをコードすると実証。 bicyclic intermediate→(S)-DNPA -- [PMID:22489461](2012) Med-ORF12のポリクロナール抗体を作成し、発現パターンを調査。 medermycinの蓄積に一致し、後期静止期に高い発現量を示した。

Sequence

>C-3 ketoreductase [stereospecific keto reductase]
MSGTGRPTVAVIGAGTIGLGWITLFLGHGLDVRVNSRRPEARSVVLEGLRTFVPTLPGGA
GLDPEELLERITFEPDVARAVEGVDVVQENAPEDLPLKQELYRLIGEAAPGRALLLSSTS
TLLPDAMGERMKDSGRLVVGHPFNSPHIVPLVEVVGGERTDPDAIARAVGFYRSVDRTPV
VLRRPIARFVANRLQSALLQESIHLVREGVVTMEELDTVVTRSIGLRWSTIGPFHSFHLG
GGEGGLRKWLGHLGVGLEKSWAELGSPRMTEETIDALGSEAERLFGGTTYAHLCRERDLR
HRAVLDALAAVEAERGEAEPGSAS

>C-3 ketoreductase [stereospecific keto reductase]
ATGAGCGGAACCGGCCGGCCCACCGTGGCGGTGATCGGCGCGGGGACGATCGGCCTCGGG
TGGATCACCCTGTTCCTCGGACACGGACTGGACGTGCGGGTGAACAGCCGCCGTCCCGAA
GCGCGGAGCGTCGTCCTGGAGGGCCTGCGGACCTTCGTGCCCACGCTGCCCGGCGGCGCC
GGACTCGACCCCGAGGAGCTCCTGGAACGGATCACCTTCGAGCCCGACGTGGCCCGCGCC
GTGGAGGGCGTCGACGTCGTCCAGGAGAACGCCCCGGAGGACCTGCCGCTCAAGCAGGAG
CTGTACCGCCTGATCGGCGAGGCCGCTCCGGGGCGGGCGCTGCTGCTGTCGTCGACCTCG
ACCCTGCTGCCCGACGCCATGGGCGAGCGGATGAAGGACTCCGGCCGTCTGGTCGTCGGG
CACCCCTTCAACTCCCCCCACATCGTTCCTCTGGTGGAGGTCGTCGGAGGCGAGCGGACG
GACCCGGACGCGATCGCGCGGGCGGTCGGCTTCTACCGCTCGGTGGACCGGACGCCCGTC
GTGCTGCGCCGGCCGATCGCCCGGTTCGTCGCGAACCGGCTGCAGTCCGCGCTGCTCCAG
GAGAGCATCCACCTCGTCCGTGAGGGCGTGGTGACGATGGAGGAGCTGGACACCGTCGTG
ACGCGGTCGATCGGCCTGCGGTGGTCCACGATCGGCCCGTTCCACTCCTTCCATCTCGGG
GGCGGGGAGGGCGGTCTGCGGAAGTGGCTGGGCCATCTCGGCGTCGGCCTCGAGAAGAGC
TGGGCCGAGCTCGGTTCGCCCCGGATGACCGAGGAGACGATCGACGCCCTCGGCTCGGAG
GCCGAACGTCTCTTCGGCGGGACCACGTACGCGCACCTGTGCCGGGAGCGCGACCTGCGG
CACCGCGCGGTGCTCGACGCGCTGGCGGCGGTCGAGGCGGAGCGCGGAGAAGCCGAGCCC
GGGAGCGCGTCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	Eukaryota
TMHMM	Transmembrane 1

Medem_00230 Medem_00250