Medem_00250 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	24,129 / 24,905 / + [in whole cluster] 24,129 / 24,905 / + [in contig]
Location	24129..24905 [in whole cluster] 24129..24905 [in contig]
Type	CDS
Length	777 bp (258 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative transcriptional regulator
Product (GenBank)	pathway-specific transcriptional activator (SARP)
Gene
Gene (GenBank)	med-ORF11
EC number
Keyword	SARP family
Note
Note (GenBank)	GTG start codon
Reference	ACC Q7WT17 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF11: Pathway-specific transcriptional activator 配列解析のみ。actII-ORF4 homologue. actII-ORF4に見られる珍しいTTA codonは、med-orf11にはない。
Related Reference	ACC P46106 NITE Actino_00160 PmId [1878971] The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces. (Cell. , 1991) [7683365] Stationary-phase production of the antibiotic actinorhodin in Streptomyces coelicolor A3(2) is transcriptionally regulated. (Mol Microbiol. , 1993) comment Blast 10th, id44%, 3e-49 Streptomyces coelicolor_actII-ORF4 Probable actinorhodin operon activatory protein [Actino_00160]transcriptional activator -- [PMID: 1878971](1991) actinorhodin biosynthetic gene clusterのactII 領域の解析論文。 actII-ORF4導入でactinorhodin産生増加。 actII-ORF4破壊でactinorhodin非産生。 actII mutant strainでは、act生合成遺伝子転写物がなくなったり減ったりした(unpublished)。 actIII geneの転写にはactII 領域が同時に存在することが必要(Hallam et al. 1988)。 actII-ORF4にはTTA codonが含まれているため、翻訳にはBldAが必要。 -- [PMID:7683365](1993) abstract 対数期→静止期でactII-ORF4転写(↑) 同時に、actIII, actVI-ORF1転写(↑)、actinorhodin産生(↑) 対数期でmulticopy plasmidによってactII-ORF4を増やすと、 →actII-ORF4 mRNA(↑) →actIII 転写、actinorhodin産生をもたらす

Sequence

>putative transcriptional regulator [pathway-specific transcriptional activator (SARP)]
MKFHLLGPFEITTEDGRSCIPPTPKASQVLALLLSRPREIVPVDAFIQELWAENPPRSAL
TTLQTYIYHLRKILVGEGLAGTGERILVTRSPGYLIDPTGHGVDTREFEELVKSGRRAAD
RGHLELAARTLGDALDMWTGPALANISTGRVLTGYTMYLEELRVRAVEQRIEALKQLGNE
RDLIPELRALIQQYPLNEWFHAQLIESLNNCGRRAEALKVYRDLWRSLSDELGIEPSPDV
QGFHHEVLNPVARTVRAA

>putative transcriptional regulator [pathway-specific transcriptional activator (SARP)]
GTGAAATTCCATCTCCTCGGCCCCTTCGAGATCACCACGGAGGACGGCCGGTCGTGCATC
CCGCCCACCCCGAAGGCCAGTCAGGTCCTCGCCCTGCTGCTCTCCCGGCCCCGGGAGATC
GTGCCGGTCGACGCGTTCATCCAGGAACTCTGGGCCGAGAACCCCCCGCGCAGCGCCCTG
ACCACCCTGCAGACGTACATCTACCACCTGCGCAAGATCCTGGTCGGCGAGGGCCTCGCC
GGAACCGGGGAACGGATCCTCGTCACCCGCAGCCCCGGCTACCTCATCGACCCCACCGGA
CACGGCGTGGACACCCGGGAGTTCGAGGAGCTCGTGAAGTCCGGACGGCGGGCCGCCGAC
CGGGGCCACCTCGAACTGGCCGCCCGCACCCTGGGCGACGCCCTCGACATGTGGACCGGC
CCGGCCCTGGCCAACATCTCCACCGGCCGCGTCCTCACCGGCTACACCATGTACCTGGAG
GAGCTGCGGGTGCGCGCCGTCGAGCAGCGCATCGAGGCGCTCAAGCAGCTCGGGAACGAG
CGCGACCTCATCCCCGAACTGCGCGCGCTCATCCAGCAGTACCCGCTCAACGAGTGGTTC
CACGCCCAGCTCATCGAGTCGCTCAACAACTGCGGCCGGCGGGCCGAGGCCCTCAAGGTC
TACCGCGACCTGTGGCGCTCGCTCTCCGACGAACTGGGCATCGAGCCGTCGCCCGACGTG
CAAGGCTTCCATCACGAGGTACTGAACCCGGTCGCGCGGACCGTCCGCGCGGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) PF00486 Trans_reg_C SM00862 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) SM01043 BTAD PF03704 BTAD IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Medem_00240 Medem_00260