Medem_00250 Medem_00250   Medem_00270 Medem_00270

Medem_00260 : CDS information

close this sectionLocation

Organism
StrainAM-7161
Entry nameMedermycin
Contig
Start / Stop / Direction24,919 / 25,359 / + [in whole cluster]
24,919 / 25,359 / + [in contig]
Location24919..25359 [in whole cluster]
24919..25359 [in contig]
TypeCDS
Length441 bp (146 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)unknown (ActVI-A homologue)
Gene
Gene (GenBank)med-ORF10
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003)
[26541165] Expression, Crystallization and Preliminary X-ray Diffraction Analyses of Med-ORF10 in the Biosynthetic Pathway of an Antitumor Antibiotic Medermycin. (Protein J. , 2015)
comment
entire medermycin(med) biosynthetic gene clusterの報告。
Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。

ORF9: Unknown(Tailoring)

配列解析のみ。actVI-ORFA homologue.
fren-ORFX、gra-ORF31、mtmX、dpsHも同じfamily member.
遺伝子機能はまだ推測だが、与えられたclusterで生合成酵素の効率を明らかに調節する。
act clusterでの提唱[PMID: 10805574]を参照している。
Related Reference
ACC
Q53925
NITE
Actino_00020
PmId
[17227452] Possible involvement of ActVI-ORFA in transcriptional regulation of actVI tailoring-step genes for actinorhodin biosynthesis. (FEMS Microbiol Lett. , 2007)
comment
Blast 5th, id51%, 5e-32
Streptomyces coelicolor_ORFA(actVI-ORFA)
Hydroxylacyl-CoA dehydrogenase
[Actino_00020]hypothetical protein

actVI-ORFA mutantはactinorhodin産生減少、中間体(S)-DNPAとshunt産物(S)-NHABを蓄積。相補で回復。
また、同じmutantでactVI-ORF1発現量が減少。相補で回復。
よってActVI-ORFAは、post-PKS tailoring enzymeであるactVI-ORF1の発現を通してactinorhodin産生に影響を与えるregulatory roleを持つ可能性あり。

close this sectionSequence

selected fasta
>hypothetical protein [unknown (ActVI-A homologue)]
MSTHRVQGDLYSRVQQFHAAQMQALDSGRFDEYADTFTEDGEFRHSPGRPAAHTRAGIAK
ELAAFHQERFAGEPVQRRHWFNMLNVQEQADGTIHATFYVLALLTREGERVPEIAPSCLV
RDVLVEEDGRLLTRSRRVEHDHLPES
selected fasta
>hypothetical protein [unknown (ActVI-A homologue)]
ATGTCCACCCACCGAGTCCAGGGAGACCTGTACAGCCGGGTGCAGCAGTTCCACGCGGCG
CAGATGCAGGCGCTCGACAGCGGCCGGTTCGACGAGTACGCCGACACCTTCACCGAGGAC
GGCGAGTTCCGGCACTCGCCCGGCAGGCCGGCCGCCCACACCCGGGCCGGCATCGCCAAG
GAGCTCGCGGCGTTCCACCAGGAGCGCTTCGCCGGCGAACCCGTCCAGCGCCGCCACTGG
TTCAACATGCTGAACGTCCAGGAGCAGGCCGACGGCACCATCCACGCCACCTTCTACGTC
CTGGCGCTGCTGACCCGGGAGGGCGAGCGCGTCCCCGAGATCGCCCCCAGCTGCCTGGTC
CGGGACGTCCTCGTCGAGGAGGACGGCCGCCTCCTCACCCGCTCCCGCCGGGTGGAGCAC
GACCACCTGCCGGAATCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
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