Medem_00290 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	28,272 / 27,628 / - [in whole cluster] 28,272 / 27,628 / - [in contig]
Location	complement(27628..28272) [in whole cluster] complement(27628..28272) [in contig]
Type	CDS
Length	645 bp (214 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	pyran ring cyclase/possible exporter (ActVI-3 homologue)
Gene
Gene (GenBank)	med-ORF5
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q7WT13 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment entire medermycin(med) biosynthetic gene clusterの報告。 Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。 ORF5: pyran ring cyclase/possible exporter 配列解析のみ。 actVI-ORF3 homologue.
Related Reference	ACC Q9ADD7 NITE Actino_00050 PmId [10805574] Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis. (J Antibiot (Tokyo). , 2000) [12421300] Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. (Mol Microbiol. , 2002) comment Blast 2nd, id39%, 2e-31 Streptomyces coelicolor_SCO5074 Putative dehydratase [Actino_00050]hypothetical protein_actVI-ORF3 -- [PMID: 10805574](2000) ORF3破壊株でもactinorhodin産生あり。wildの55%。 -- [PMID:12421300](2002) 菌糸体抽出液のプロテオミクス解析。全長ActVI-ORF3は検出されない。 N末31 or 34aaが切断されたformで検出され、SignalP予測もこれに一致する。 whole cell extractよりexternal SDS extractで検出される。よって、ActVI-ORF3は細胞壁マトリックスに排出されると示唆される。

Sequence

>hypothetical protein [pyran ring cyclase/possible exporter (ActVI-3 homologue)]
MKKKSAAGIAAALSISAATAMLSVASPANATGPGTSTSSTVGYVCQSRYDGAWFPINGYS
RGFTTTAPAQVGKGVAFNVSFDPAPILAVGDYNKELTDIEVAYKVSPEARVLGYKLVGGS
NLGTARFHVERRGAEFVVTSPDTLPGGVEFDLPTLVVVRAPSTAGTVTTAPGGSSFENRS
FGWNRLHPANNQWDPFQCYANPATPVVFSTTNVA

>hypothetical protein [pyran ring cyclase/possible exporter (ActVI-3 homologue)]
ATGAAGAAGAAGAGCGCGGCGGGGATCGCCGCCGCCCTGAGCATCTCGGCGGCCACCGCA
ATGCTGAGCGTCGCGTCTCCGGCGAACGCCACCGGCCCGGGGACCAGCACCTCGTCGACC
GTCGGCTACGTGTGCCAGAGCCGCTACGACGGCGCGTGGTTCCCGATCAACGGGTACTCC
CGGGGCTTCACCACCACGGCTCCGGCGCAGGTCGGCAAGGGCGTCGCGTTCAACGTGTCG
TTCGACCCCGCGCCGATCCTGGCGGTGGGCGACTACAACAAGGAGCTCACCGACATCGAG
GTCGCCTACAAGGTGTCGCCCGAGGCCCGCGTCCTCGGCTACAAGCTGGTCGGCGGCAGC
AACCTCGGCACGGCCAGGTTCCACGTCGAACGCCGCGGCGCCGAGTTCGTCGTCACCTCG
CCCGACACCCTGCCCGGCGGCGTCGAGTTCGATCTGCCCACCCTCGTGGTCGTGCGCGCG
CCCTCCACCGCGGGCACGGTCACGACCGCCCCCGGCGGTTCGAGCTTCGAGAACCGCTCC
TTCGGCTGGAACCGTCTGCACCCGGCGAACAACCAGTGGGACCCGTTCCAGTGCTACGCC
AACCCGGCGACGCCGGTCGTGTTCAGCACGACGAACGTCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Medem_00280 Medem_00300