Medem_00270 Medem_00270   Medem_00290 Medem_00290

Medem_00280 : CDS information

close this sectionLocation

Organism
StrainAM-7161
Entry nameMedermycin
Contig
Start / Stop / Direction26,416 / 27,546 / + [in whole cluster]
26,416 / 27,546 / + [in contig]
Location26416..27546 [in whole cluster]
26416..27546 [in contig]
TypeCDS
Length1,131 bp (376 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative C-glycosyltransferase
Product (GenBank)C-glycosyl transferase
Gene
Gene (GenBank)med-ORF8
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003)
comment
entire medermycin(med) biosynthetic gene clusterの報告。
Streptomyces coelicolor CH999でmed clusterを発現してmedermycin産生を確認している。

ORF8: C-Glycosyl transferase

系統樹で、UrdGT2, Gra-ORF14, Med-ORF8, LanGT2 and SnoTはO-glycosyltransferasesと別になる。
UrdGT2はC-glycosylation活性が既に実証されている。

alignmentを取ると、構造研究で明らかになっている高保存Asp(有望なcatalytic base)が主にO-glycosyltransferasesに制限され、UrdGT2, Gra-ORF14などには保存が見られなかった。
この残基（の不在）はC-glycosylationのための特異性を決定するかもしれない。
Related Reference
ACC
Q9ZGC0
NITE
Lando_00200
PmId
[15977274] LanGT2 Catalyzes the First Glycosylation Step during landomycin A biosynthesis. (Chembiochem. , 2005)
[21513888] Rational design of an aryl-C-glycoside catalyst from a natural product O-glycosyltransferase. (Chem Biol. , 2011)
comment
Blast 4th, id56%, 1e-101
Streptomyces cyanogenus_lanGT2
Glycosyl transferase homolog
[Lando_00200]D-olivose glycosyltransferase

---
[PMID: 15977274](2005) abstract
lanGT2不活化mutantの産物は糖側鎖を欠損。
よってLanGT2がhexasaccharide生合成においてpriming glycosyl transfer(polyketide骨格のO-8へのD-olivoseの付着)を触媒することを示す。

lanGT2 mutantでurdGT2を発現すると新規C-glycosylated angucyclineが産生されるのを確認している。
よってLanGT2とUrdGT2は似ているが異なる機能を持つ。

---
[PMID: 21513888](2011)
O-glycosyltransferase LanGT2を操作して、UrdGT2のようなC-Glycosylation活性を持つようにしている。
LanGT2, UrdGT2の反応メカニズムを提唱。
ACC
Q9RPA7
NITE
Urd_00200
PmId
[10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000)
[12696864] The C-Glycosyltransferase UrdGT2 is unselective toward d- and l-configured nucleotide-bound rhodinoses. (J Am Chem Soc. , 2003)
[17640665] Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin. (J Mol Biol. , 2007)
comment
Blast 5th, id50%, 3e-91
Streptomyces fradiae_urdGT2
Glycosyl transferase
[Urd_00200]D-olivose C-glycosyltransferase

[PMID: 10658661](2000)
urdGT2が削除されたmutantは、urdamycin Aの代わりに、C-C connected deoxysugar moietyを欠いたtetracyclic angucyclinonesであるurdamycin I, urdamycin J and urdamycin Kを産生した。したがって、UrdGT2はNDP-D-olivoseのC-glycosyltransferを触媒する。

構造解析など続報あり。

close this sectionSequence

selected fasta
>putative C-glycosyltransferase [C-glycosyl transferase]
MRILFVAGPSPATVFGLASLAVAARQAGHRVIVAGTEELVPHITGVGLPGAAVTPRTWES
LIVTDRSGGAVPRMPRGVEPERRHHGGWFGRLAAESLHGLRELAAHWRPGLVVGGSMSYA
APLLAAELGVPYVRQAWDLYDTRGVDPYAAEELRPELAALGLDRLPEPDLRVDVCPPSLR
PPDADPAQPMRWIPYNRQQELEPWMYGRGDRPRVCVTSGSRETEANRDFLGRLVADLAAF
DAEILVAAPEEAAARLRETAGRVRIGWMPLDVVAPTCDLLVHHGGGVTGMTALAAGTPQL
LLSRWDIFASTMRRTAERGAARVLFPEDTSPEAVLRACRELLEEPGPRKRSAEIAAEIAA
LPGPAEVVTALERLAA
selected fasta
>putative C-glycosyltransferase [C-glycosyl transferase]
ATGAGGATCCTCTTCGTCGCCGGCCCCAGCCCGGCGACCGTCTTCGGGCTCGCGTCCCTG
GCCGTCGCCGCACGTCAGGCGGGACACCGGGTGATCGTGGCCGGGACGGAGGAGCTCGTC
CCGCACATCACCGGCGTCGGCCTGCCCGGCGCGGCGGTCACGCCACGCACCTGGGAGAGC
CTGATCGTCACCGACCGGTCCGGCGGCGCGGTGCCCCGGATGCCCCGGGGCGTCGAGCCG
GAACGCCGCCACCACGGCGGCTGGTTCGGCCGGCTGGCCGCCGAGAGCCTGCACGGCCTG
CGCGAACTCGCCGCCCACTGGCGCCCCGGACTGGTCGTCGGCGGCAGCATGTCGTACGCG
GCCCCGCTGCTCGCGGCCGAGCTGGGCGTCCCGTACGTCCGCCAGGCCTGGGACCTGTAC
GACACCCGGGGGGTCGACCCGTACGCGGCGGAGGAGCTCCGCCCGGAGCTGGCCGCGCTG
GGGCTCGACCGGCTCCCCGAGCCGGACCTCCGGGTCGACGTCTGCCCGCCGAGCCTGCGG
CCCCCGGACGCCGACCCGGCGCAGCCGATGCGCTGGATCCCGTACAACCGGCAGCAGGAG
CTCGAACCGTGGATGTACGGCCGGGGCGACCGGCCCCGGGTGTGCGTGACCTCGGGCAGC
CGCGAGACGGAGGCCAACCGGGACTTCCTGGGCCGGCTGGTCGCGGACCTCGCCGCCTTC
GACGCGGAGATCCTCGTCGCCGCCCCGGAGGAGGCCGCGGCCCGGCTGCGGGAGACCGCC
GGGCGGGTACGGATCGGGTGGATGCCGCTCGACGTCGTCGCCCCGACCTGCGACCTCCTC
GTCCACCACGGCGGGGGAGTCACCGGGATGACCGCGCTCGCCGCCGGGACGCCCCAACTG
CTGCTGTCCCGCTGGGACATCTTCGCCTCGACCATGCGCCGCACGGCCGAGCGGGGTGCG
GCGCGGGTCCTCTTCCCCGAGGACACCTCGCCCGAGGCCGTGCTGCGGGCGTGCCGGGAG
CTCCTGGAGGAGCCCGGCCCCCGGAAGAGGTCCGCAGAGATCGCCGCCGAGATCGCCGCC
CTGCCCGGGCCGGCGGAGGTGGTCACGGCCCTGGAGCGGCTGGCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-135] 1.1e-06 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[172-264] 1e-24 PF06722
PF06722   DUF1205
SignalP
[1-27] 0.98 Signal
Eukaryota   
[1-23] 0.993 Signal
Bacteria, Gram-negative   
[1-24] 0.982 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Medem_00270 Medem_00270   Medem_00290 Medem_00290
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