Meili_00310 Meili_00310   Meili_00330 Meili_00330

Meili_00320 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameMeilingmycin
Contig
Start / Stop / Direction99,015 / 96,121 / - [in whole cluster]
99,015 / 96,121 / - [in contig]
Locationcomplement(96121..99015) [in whole cluster]
complement(96121..99015) [in contig]
TypeCDS
Length2,895 bp (964 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)LuxR-family transcriptional regulator
Gene
Gene (GenBank)meiR
EC number
Keyword
Note
Note (GenBank)
  • similar to AveR
Reference
ACC
PmId
[20348291] Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification. (Appl Environ Microbiol. , 2010)
comment
Meilingmycin Biosynthesis Gene Clustersを同定した文献。

Table1.
MeiR(964 a.a.): LuxR family transcriptional regulator

meiR に関しては、deletionやcomplementationの実験等はしていない。
Related Reference
ACC
Q9S0N8
NITE
Aver_00010
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
comment
<Streptomyces avermitilis_aveR, BLAST 1; id=50%>

--
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

--
[PMID: 19148632](2009)abstract
aveRを不活化することによって、avermectinの生産が完全に無くなること、また、avermectin中間体をいかなるavermectin派生体にも変換できないこと、故にAveRは、avermectinの生合成遺伝子と修飾遺伝子を共に発現調節するpositive regulatorであることが示唆されている。さらに、大量のAveRによって、avermectinは完全に損失することから、avermectinの生産に際してはAveRに最高許容限界濃度があることが示されている。

--
[PMID: 20012992](2010)abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [LuxR-family transcriptional regulator]
MLIDRSAHIELAEATLHESAAGDFRLLVAEGGPGCGKSEFLKRFLGIAAESGAVVLDAPG
LPCESGTPLGLVRRLAEHPALPDRTRERLRHAPSQDGPRAVERLCGALCALSASAPLVIA
VDDLHHADQASLRQLLHIAHHARSAKILLIGTESPSGMDSPYSGMERPYGTERPYGRERP
CGRGGDPLLGAELLRHPGFRRITLDPLGPESVARLAATLGDQPVDGRLGAECYALTGGNP
LLLRALLQDRWAVPDTGGRAPRPTAGGPFHQAVVACLARTGTPIRDVAGALAVLGPSATP
ALLSRLLGLDATAVDRALQVLELSGLTDGHRCRHPVVAAAALDHLDAADRRLLHERGALL
LHEQGARATEVADHLLAAQPTVPSWAVAVLRDAATQALVDDDAPAAIRYLDLACRACQEP
PQRAATRIALAAATWRVNPSAAEHHLGDTLADLHTGTPTGSDAALLVRLLISCGRLEEAG
RARARLQTADHPGNDPDNGPGPGPDPGAGPDTDTDGGAVRPSVVHPCPWPIASDESRPRP
PSDTAAPREPWDETPGPGAAAAVWGIPGSGTSEAAADEAERRLRACPLLDGTLAVVANAV
RTLLRAGRTADADEWCHRLLKEATARRSPGWRAEFLAILAEIALRKGVLTDAEECARQAL
ACLPGRGGSVLVGGPLACQVHAYTAMGRYDDATRVLHHPVPGALFQSVYGLAYLRARGHY
HLATDHCRAALGDFLAAGRMAQRWGLDHPAVLPWRTDSAQAFLRLGERTRAARMITEQLS
CAHLWSDPHTRGVSLRLRARVAAAPERPHLLTQAVEVLQRSGDRLELARALADLGAAYRS
VGESIRANNVRRRAWRMAEECGAKALCDAILPWQAMCGGDRPDLPGRDQGAGLSESEMRV
AILAANGNSNREIALQLCVTMSTVEQHLTRVYRKLRISRRQQLPKSLIGPVGTPATDRRE
LSSL
selected fasta
>putative LuxR family transcriptional regulator [LuxR-family transcriptional regulator]
ATGCTCATTGACCGGAGCGCTCACATAGAGCTGGCGGAAGCGACGCTTCATGAGAGTGCG
GCAGGCGATTTCCGGCTGCTGGTCGCCGAGGGCGGGCCGGGGTGCGGCAAAAGCGAATTC
CTGAAGCGGTTCCTGGGGATCGCGGCCGAATCCGGTGCCGTGGTCCTCGACGCGCCCGGC
CTGCCATGCGAGAGCGGCACCCCGCTCGGCCTCGTACGACGGCTGGCCGAGCACCCGGCG
CTGCCGGACCGGACCCGCGAGCGGCTGCGACACGCCCCGTCACAGGACGGGCCCCGGGCG
GTGGAGCGGCTGTGCGGGGCGCTGTGCGCGCTCTCCGCGTCCGCACCGCTGGTGATCGCG
GTCGACGATCTGCACCACGCGGACCAGGCGTCGCTCCGGCAGCTGCTGCATATCGCCCAC
CACGCTCGCTCGGCGAAGATCCTGCTGATCGGCACGGAGAGCCCGTCCGGCATGGACAGC
CCGTACAGCGGCATGGAGCGCCCGTACGGCACGGAGCGACCGTACGGTCGGGAGCGCCCT
TGCGGTCGAGGCGGCGACCCGCTGCTCGGCGCCGAACTGCTGCGCCACCCCGGTTTCCGA
CGCATCACGCTCGACCCGCTCGGCCCCGAAAGCGTGGCCAGGCTGGCCGCCACGCTCGGC
GACCAGCCGGTGGACGGCCGCCTCGGCGCCGAGTGCTACGCACTGACCGGCGGCAATCCG
CTGTTGCTGCGGGCCCTGCTGCAGGATCGGTGGGCGGTGCCGGACACCGGTGGCCGGGCG
CCTCGGCCCACCGCCGGGGGACCCTTCCACCAGGCGGTGGTGGCCTGCCTGGCCCGCACC
GGGACGCCCATCCGCGACGTCGCCGGCGCACTGGCCGTACTCGGGCCGTCCGCCACACCC
GCGCTGCTGAGCCGACTGCTGGGACTGGACGCGACAGCGGTCGACCGCGCCCTCCAGGTG
CTGGAGCTGAGCGGCCTGACGGATGGGCACCGCTGCCGGCACCCGGTCGTCGCGGCGGCA
GCTCTGGACCATCTGGATGCCGCCGACCGGCGGTTGCTGCACGAGCGCGGCGCCCTGCTG
CTGCACGAGCAGGGCGCCCGGGCCACCGAGGTCGCCGACCACCTGCTGGCCGCCCAGCCC
ACGGTCCCCTCCTGGGCTGTCGCCGTACTGCGCGACGCCGCCACCCAGGCCCTGGTCGAC
GACGACGCACCTGCGGCGATCAGGTATCTGGACCTCGCCTGCCGCGCCTGCCAGGAGCCG
CCCCAGCGGGCCGCGACCCGGATCGCGCTCGCCGCCGCCACCTGGCGGGTCAACCCCTCC
GCCGCCGAACACCACCTCGGTGACACGCTCGCCGACCTGCACACCGGCACGCCGACGGGC
AGTGACGCGGCCCTGCTCGTACGGCTGCTGATCAGCTGTGGCCGACTGGAGGAGGCGGGC
CGGGCACGGGCCCGGCTGCAGACGGCCGACCACCCCGGCAACGACCCTGACAACGGCCCC
GGACCGGGCCCGGACCCGGGCGCGGGCCCGGACACGGACACAGACGGGGGCGCGGTGCGG
CCGTCGGTGGTGCACCCGTGCCCCTGGCCCATCGCGTCCGACGAGAGCCGGCCGCGGCCC
CCGTCCGACACCGCCGCCCCCCGCGAGCCCTGGGACGAGACTCCCGGCCCCGGCGCGGCC
GCCGCCGTCTGGGGCATCCCCGGCTCCGGCACCAGCGAGGCCGCCGCGGACGAGGCCGAA
CGGCGGCTGCGCGCCTGCCCGCTGCTGGACGGCACGCTGGCGGTGGTGGCCAACGCCGTA
CGGACCCTGCTGCGGGCCGGCCGTACGGCCGATGCCGACGAGTGGTGCCACCGGCTGCTC
AAGGAGGCGACGGCCCGCCGCTCGCCCGGCTGGCGGGCGGAGTTCCTTGCCATCCTTGCC
GAGATCGCCCTGCGCAAGGGCGTGCTGACGGACGCCGAGGAGTGCGCCCGGCAAGCGCTG
GCCTGCCTGCCCGGCCGCGGTGGCAGTGTGCTCGTCGGAGGTCCGCTCGCCTGCCAGGTC
CATGCCTATACCGCCATGGGTCGGTACGATGACGCGACCCGTGTGCTGCACCACCCGGTG
CCGGGCGCGCTGTTCCAGAGCGTGTACGGCCTGGCGTATCTGCGCGCCCGCGGCCACTAC
CACCTGGCCACCGACCACTGCCGCGCCGCACTCGGCGACTTCCTGGCCGCCGGCCGTATG
GCACAGCGCTGGGGCCTGGACCACCCCGCCGTGCTGCCCTGGCGCACCGACAGCGCCCAG
GCGTTCCTGCGACTCGGCGAGCGCACCAGGGCGGCGCGGATGATCACGGAGCAGCTGTCC
TGTGCCCACCTGTGGAGCGATCCGCACACCCGTGGCGTCTCGCTCAGGCTCCGGGCCCGG
GTCGCCGCGGCGCCCGAGCGCCCGCATCTGCTCACCCAGGCAGTCGAGGTGCTGCAGCGG
TCCGGGGACCGGCTCGAACTCGCCCGGGCGCTGGCCGACTTGGGCGCCGCATACCGGAGC
GTCGGCGAGTCGATCAGGGCCAACAATGTGCGGCGCAGGGCGTGGCGGATGGCCGAGGAG
TGCGGCGCCAAGGCGCTGTGCGACGCCATTCTGCCCTGGCAGGCGATGTGCGGCGGGGAC
CGGCCGGACCTCCCTGGCCGTGACCAGGGAGCGGGACTGAGCGAGTCGGAAATGCGTGTG
GCCATCCTCGCCGCCAACGGCAACTCGAACCGTGAGATAGCGCTGCAGCTGTGCGTGACG
ATGAGCACGGTCGAACAGCATCTGACACGGGTGTACCGCAAGCTGCGCATCAGCCGCCGC
CAGCAACTGCCGAAGAGTCTGATCGGTCCGGTGGGGACACCGGCGACGGACCGCAGAGAG
CTGTCCTCGCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[892-943] 7.79999999999999e-11 PF00196
PF00196   GerE
[907-934] PS00622
PS00622   HTH_LUXR_1
[893-907] 1.10000029896148e-07 PR00038 [907-923] 1.10000029896148e-07 PR00038 [923-935] 1.10000029896148e-07 PR00038
PR00038   HTHLUXR
[890-947] 3.60000433965927e-17 SM00421
SM00421   HTH_LUXR
IPR011990 Tetratricopeptide-like helical (Domain)
[569-901] 8.00000000000001e-18 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[902-943] 7.59999999999999e-16 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[871-949] 3.59999643435987e-14 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Meili_00310 Meili_00310   Meili_00330 Meili_00330
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