Merid_00010 : CDS information

Location

Organism	Streptomyces violaceusniger
Strain	DSM 4137
Entry name	Meridamycin
Contig	DQ885223
Start / Stop / Direction	7,793 / 12,610 / + [in whole cluster] 7,793 / 12,610 / + [in contig]
Location	7793..12610 [in whole cluster] 7793..12610 [in contig]
Type	CDS
Length	4,818 bp (1,605 aa)

Annotation

Category	1.2 NRPS
Product	non-ribosomal peptide synthetase
Product (GenBank)	MerP
Gene
Gene (GenBank)	merP
EC number
Keyword	L-pipecolate
Note
Note (GenBank)	non-ribosomal peptide synthetase
Reference	ACC A0FCL0 PmId [17159202] Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. (Microbiology. , 2006) comment meridamycin biosynthetic gene clusterを同定した文献。 merP(1,605a.a): Non-ribosomal peptide synthetase merP自体の機能実験は行っていないが、相同性よりpipecolate unitをpolyketide chainに付加し、その後に生じるmacrocycle形成に関与することが提唱されている。
Related Reference	ACC Q1WEL0 PmId [16806745] Isolation and characterization of meridamycin biosynthetic gene cluster from Streptomyces sp. NRRL 30748. (Gene. , 2006) comment 2nd, id93%, 0.0 Streptomyces sp. NRRL 30748_merP NRPS for pipecolate incorporation Streptomyces sp. NRRL 30748でのmeridamycin生合成gene cluster同定。 MerP(1572aa): NRPS for incorporating pipecolic acid (single module with 4 domains: C, A, T, C) この株にはlysine cyclodeaminaseをコードする遺伝子が見当たらないので、pipecolate合成はrapamycin and FK506/FK520生合成で述べられている経路とは異なる。 merP破壊でmeridamycin非産生。
	ACC Q54298 NITE Rapam_00130 PmId [9266694] The pipecolate-incorporating enzyme for the biosynthesis of the immunosuppressant rapamycin--nucleotide sequence analysis, disruption and heterologous expression of rapP from Streptomyces hygroscopicus. (Eur J Biochem. , 1997) comment 3rd, id45%, 0.0 Streptomyces hygroscopicus_rapP pipecolate incorporating enzyme putative pipecolate-incorporating enzymeをコードするrapPを同定、配列解析。 rapP破壊mutantでrapamycin非産生。 rapPをE.coli, S.coelicolorで発現、精製。 recombinant RapPの翻訳後修飾を確認。
	ACC Q9KID8 NITE Asco_00160 PmId [15835888] Elucidating the substrate specificity and condensation domain activity of FkbP, the FK520 pipecolate-incorporating enzyme. (Biochemistry. , 2005) comment 8th, id46%, 0.0 Streptomyces hygroscopicus subsp. ascomyceticus_fkbP FkbP FkbPは4つのdomainのNRPS。 AA挿入の後、鎖を環化するのにもおそらく役立つ。 recombinant FkbPの発現と精製。FkbPはvirtoでphosphopantetheinylateされていたことを示し、そのPCP domainはL-pipecolic acidと共にうまくロードされた。 Site-directed mutagenesisで、N末adenylation domainがtransfer reactionを触媒することを確認。 FkbPのadenylation domainはpipecolate取り込みのための一次選択フィルターである。

PKS/NRPS Module

15
16

C	40..338

A	524..967

PCP	1056..1123

C	1144..1437

Sequence

>non-ribosomal peptide synthetase [MerP]
MGHSGAPKSRGLSAAMSNLFERTRRNESTGIVPVDRGRELRASFAQQRLWFLDQLEPGNA
SYNLPFAVRVRGRLDIPHLSRALSLVVARHEALRTTFGEAGGQPVQRIQPPAPVPVRLEP
VPGDSEEERLAEVRRLAGAEITEPFDLSTGPLLRAKALRLDEHDHVLLLTVHHVATDAWS
QGIVVRELSAAYEALDAGREPVLPPLPVQYADYAEWERDWLSGPTLRRQLDYWTKRLDGM
APALELPTDRPRPSVARQEGDAVRWELPAELIRAARRLGAGENATLYMTLLAAFQLVLGR
YVDSDDITVGTPVANRGRAEVEGLIGFFVNTVVLRTDLSGDPTFRQLLGRVRDMAAGAFA
HGDLPFEYLVEQVHPERDLSRNPLVQVLFQMINVPAERLALPGARTEPYDHGGILTRMDL
EVHLVEIEDGVLGHIVFSKALFDTSTIERLLHHVTVVLRGVLAEPDRCISEISPLDEAER
TKVLEEFNATTGPVPAGSLPALFTAQVERRPDAVAVISGGDRVTYAELDQRANQLAHLLA
GRGVGPEALVGLCVDRGIEMIVAILAILKLGAAYVPIDPNHPRGRVQFVLADAGVTVAVT
QQRFTGLLETPEAPGKPEVSGTHGVPGAAPGAPDAPEASGTPDAPGTPGIRLILLDAERE
PLAGQPRTPPTARPSAQNLAYVIYTSGSTGVPKGILMPATCVLNLVAWQKKALPIGPDAK
TAQFAMLTFDISLQEIFSALLYGETIVVPGEELRMDPVEFATWVHTNEIDQLFVPNVMLR
AISEEVDPHGTELAALRHLSQAGEPLSLHHDLRELCAHRPELRLHNHYGPSEAHVVTSYS
LPAEVAEWPLTAPIGRPIGNTRVYVVDRRLRPVPVGVPGELCVAGEGLARGYLGRPDLTA
ARFVADPFRGDGSRMYRSGDLVRWLPDGNLEFLGRIDDQVKIRGFRIEPGEIEAILARHQ
DVLHTAVMVREDTPADKRLVAYVVADATAADRPGRLTETLRRYVESAVPEYMVPSAFVLL
DTMPVTSGGKIDRKALPAPDLRTVLEVGYVAPRTPEEEAVCRVYTDLLGATRVGIDDDFF
ALGGHSLIATRVVARLRSALGIAVPLKAVFQQRTPRQLAATLTAAARSGPEPELPPLIPT
RRDQPIPLTFAQQQTDLFFGDVLDAGHWNIPMAVRVSGELDLDCLRRAMDLLIDRHEALR
TAFVRETDRYVQVIRPSAPVQVEVAEARDEAEASVLAGQEAARPFDLTRGPLARLRVLRL
AESDHVLVLTLHHLVTDGWSQGVLVRDLSIVYAALLRGTEPDLPPVPVQYADVASWERKW
LHGPLLRRQLDFWKRHFAGMAPAELPTDRPRAASARYESDIFRWRLPKDAVETARRLGES
SNATLYMTLLTALKVVMSARSDNQDVLVGVPTANRGRDELENTVGLVSKMLALRTEVSGA
TDFGTLLATVRDAMSDAHTHQDVPFVSVLKHIGEHTGGLGGGPAGEHTDDRVGGRAGTRL
SDDPPVKVIFQIVNTPPRPLRLTGLTAEPFLMTHPPVTVNVDMEIDLYESAEDGGLAGTV
LYSKSLFDRATIERFCDDVVAVVSTAAADPGRPVSQVWQGRGRDQ

>non-ribosomal peptide synthetase [MerP]
GTGGGGCATTCAGGCGCGCCCAAATCCCGTGGTCTCAGCGCGGCCATGAGCAATCTCTTC
GAGCGGACCAGGAGAAACGAATCCACCGGCATTGTGCCGGTCGACCGGGGCCGGGAGCTG
AGGGCGTCGTTCGCCCAGCAACGGCTGTGGTTTCTCGACCAGTTGGAACCCGGCAACGCC
TCGTACAACCTCCCCTTCGCGGTGCGGGTGCGCGGCCGCCTGGACATCCCTCATCTCTCC
CGGGCCCTTTCGCTCGTGGTCGCCCGGCACGAGGCGCTGCGCACCACCTTCGGCGAGGCC
GGCGGTCAGCCGGTGCAGCGGATCCAGCCCCCCGCCCCCGTCCCGGTGCGCCTCGAACCG
GTGCCCGGCGACTCCGAGGAGGAGCGGCTGGCCGAGGTCCGGCGGCTGGCCGGAGCCGAG
ATCACCGAGCCCTTCGACCTGAGCACCGGACCCCTGCTGCGAGCCAAGGCGCTGCGACTG
GACGAACACGACCACGTCCTGCTGCTCACGGTGCACCATGTGGCGACCGACGCCTGGTCA
CAGGGCATTGTGGTGCGTGAGCTGTCCGCCGCGTACGAGGCGCTGGACGCCGGGCGCGAG
CCGGTACTGCCCCCGCTGCCCGTGCAGTACGCCGACTACGCGGAGTGGGAGCGCGACTGG
CTGTCCGGCCCGACCCTGCGCCGCCAGCTGGACTACTGGACGAAGCGGCTGGACGGCATG
GCGCCCGCGCTGGAGCTGCCCACCGACCGGCCCAGGCCCTCGGTCGCCCGCCAGGAAGGC
GACGCGGTGCGCTGGGAGTTGCCGGCCGAACTGATCCGGGCGGCCCGCCGACTGGGCGCC
GGGGAGAACGCGACCCTCTATATGACCCTGCTGGCCGCCTTCCAACTGGTGCTGGGCCGG
TACGTGGACAGCGACGACATCACGGTGGGCACCCCCGTGGCCAACCGGGGCCGCGCCGAG
GTCGAGGGCCTCATCGGGTTCTTCGTCAACACCGTGGTGCTGCGGACCGATCTGTCCGGC
GACCCCACCTTCCGCCAACTGCTGGGCCGGGTCCGCGACATGGCGGCCGGTGCATTCGCC
CATGGCGACCTGCCCTTCGAATATCTGGTGGAGCAGGTGCACCCCGAGCGGGACCTGTCG
CGCAACCCGCTGGTCCAGGTGCTCTTCCAGATGATCAACGTACCGGCGGAGCGGCTCGCC
CTGCCCGGCGCGCGGACCGAGCCCTACGACCACGGCGGCATCCTCACTCGAATGGATCTT
GAGGTCCATCTCGTCGAGATCGAGGACGGAGTTCTCGGACACATCGTCTTCAGCAAGGCC
CTGTTCGACACGAGCACCATTGAACGGCTGCTGCACCACGTCACCGTCGTCCTCCGGGGC
GTCCTGGCCGAGCCGGACCGGTGCATCTCCGAGATCTCGCCGCTCGACGAGGCGGAGCGG
ACGAAGGTCCTGGAGGAGTTCAACGCGACCACGGGCCCCGTACCCGCCGGATCCCTGCCC
GCGCTCTTCACCGCCCAGGTCGAGCGCCGCCCCGATGCGGTGGCCGTGATCAGCGGTGGT
GACCGGGTGACCTACGCCGAGCTGGATCAGCGGGCGAACCAGCTCGCCCATCTGCTGGCG
GGACGGGGGGTCGGCCCCGAGGCCCTGGTCGGGCTCTGCGTCGATCGCGGCATCGAGATG
ATCGTGGCGATCCTCGCGATCCTCAAGCTGGGAGCGGCCTATGTGCCGATCGATCCCAAC
CACCCCCGGGGCCGCGTCCAGTTCGTCCTGGCCGACGCCGGGGTGACCGTCGCCGTCACC
CAGCAGCGTTTCACCGGCCTGCTCGAAACGCCGGAGGCACCCGGGAAGCCGGAAGTATCC
GGGACACATGGGGTGCCTGGAGCAGCACCCGGGGCGCCCGACGCGCCCGAGGCATCCGGG
ACGCCCGATGCGCCCGGGACGCCCGGCATTCGGCTGATCCTGCTTGACGCCGAGCGCGAA
CCGCTCGCCGGGCAGCCCCGGACCCCGCCCACGGCACGGCCCAGCGCCCAGAACCTCGCC
TACGTCATTTACACCTCCGGCTCCACCGGAGTCCCCAAGGGCATCCTCATGCCCGCCACC
TGTGTGCTCAACCTGGTGGCCTGGCAGAAGAAGGCCCTGCCGATCGGTCCCGACGCCAAG
ACGGCACAGTTCGCCATGCTGACCTTCGACATCTCGTTGCAGGAGATCTTCTCCGCGCTG
CTGTACGGCGAGACGATCGTCGTCCCCGGCGAGGAACTCCGCATGGACCCCGTCGAGTTC
GCCACATGGGTCCACACCAACGAGATCGACCAGCTCTTCGTCCCGAATGTGATGCTGCGG
GCGATCTCCGAGGAGGTGGATCCGCACGGCACCGAGCTGGCCGCACTGCGCCACCTCTCA
CAGGCCGGCGAACCCCTCTCCCTCCACCACGATCTGCGCGAGCTGTGCGCCCACCGCCCC
GAGCTGCGGCTGCACAACCACTACGGCCCCAGCGAAGCCCATGTGGTGACGTCGTACTCG
CTCCCCGCCGAGGTGGCCGAGTGGCCGCTCACCGCACCCATCGGCCGCCCGATCGGCAAC
ACCCGGGTGTATGTGGTCGACCGACGGCTCCGGCCCGTCCCGGTGGGGGTGCCCGGCGAG
CTGTGCGTGGCCGGAGAGGGCCTGGCCAGGGGCTATCTCGGCCGCCCGGATCTGACCGCC
GCCCGATTCGTGGCCGACCCGTTCCGGGGCGATGGATCGCGTATGTACCGCTCCGGCGAT
CTGGTGCGCTGGCTGCCCGACGGCAACCTGGAATTCCTCGGCCGGATCGACGACCAGGTG
AAGATTCGTGGCTTCCGGATCGAACCGGGCGAGATCGAGGCGATCCTCGCCCGCCACCAG
GACGTTCTGCACACGGCCGTGATGGTGCGCGAGGACACCCCCGCCGACAAGAGGCTGGTG
GCCTATGTGGTGGCCGACGCCACCGCCGCGGACCGGCCCGGCCGGCTGACCGAGACCCTG
CGTCGGTACGTCGAGTCCGCGGTGCCCGAATACATGGTGCCCTCCGCGTTCGTGCTGCTG
GACACCATGCCCGTGACCTCCGGCGGCAAGATCGACCGGAAGGCGCTGCCCGCTCCCGAT
CTGCGCACCGTGCTCGAGGTCGGCTACGTCGCCCCCCGCACCCCCGAGGAAGAGGCCGTC
TGTCGGGTCTACACGGATCTGCTCGGCGCCACCAGGGTCGGCATCGACGACGACTTCTTC
GCACTGGGCGGCCATTCCCTCATCGCCACCAGGGTGGTCGCCAGGCTCAGGTCCGCCCTC
GGTATCGCCGTACCGCTGAAGGCCGTCTTCCAGCAGCGCACCCCCCGACAGCTGGCGGCC
ACGCTCACCGCCGCGGCCCGCTCCGGTCCCGAACCCGAGCTGCCGCCGCTGATTCCCACG
CGGCGCGACCAGCCCATCCCCCTCACCTTCGCACAGCAGCAGACGGACCTCTTCTTCGGC
GATGTCCTGGACGCCGGGCACTGGAACATCCCCATGGCGGTGCGGGTGTCGGGCGAACTG
GACCTCGACTGCCTGCGCCGGGCGATGGACCTGCTGATCGACCGCCACGAGGCCCTGCGT
ACCGCCTTCGTCAGGGAAACCGACAGATACGTCCAGGTGATCCGGCCGAGCGCGCCGGTC
CAGGTGGAGGTGGCCGAGGCGCGGGACGAGGCCGAAGCCTCGGTACTGGCCGGCCAGGAG
GCGGCCCGTCCCTTCGACCTCACCCGCGGCCCGCTGGCGAGACTGCGCGTGCTGCGGCTG
GCCGAGTCCGACCATGTGCTGGTGCTCACCCTGCACCACCTGGTCACCGACGGCTGGTCC
CAGGGAGTGCTGGTCCGCGACCTGTCCATCGTGTACGCGGCACTGCTGCGCGGCACCGAA
CCCGATCTGCCACCCGTACCCGTCCAGTACGCCGATGTCGCGAGCTGGGAGCGGAAGTGG
TTGCACGGTCCGCTGCTGCGACGCCAACTCGACTTCTGGAAGCGGCATTTCGCGGGCATG
GCCCCCGCCGAACTGCCCACCGACCGGCCCCGCGCCGCATCGGCCCGCTACGAGAGCGAC
ATCTTCCGCTGGCGACTGCCGAAGGACGCAGTCGAGACCGCCCGACGGCTGGGCGAATCG
TCCAACGCCACCTTGTACATGACGCTGCTGACCGCCCTGAAGGTGGTCATGTCCGCCCGC
TCGGACAACCAGGACGTCCTCGTCGGTGTGCCCACGGCCAACCGGGGCCGGGACGAACTG
GAGAACACGGTGGGCCTCGTCTCCAAGATGCTCGCACTGCGCACCGAAGTGTCCGGCGCC
ACGGACTTCGGCACGCTGCTGGCCACGGTGCGCGATGCGATGTCCGACGCCCATACACAC
CAGGACGTGCCCTTCGTGTCCGTTCTGAAGCACATCGGCGAACACACCGGTGGCCTCGGC
GGCGGCCCCGCTGGTGAGCACACCGATGACCGCGTCGGCGGCCGGGCCGGGACGCGGCTG
TCGGACGATCCACCGGTGAAGGTGATCTTTCAGATCGTCAACACCCCGCCGCGGCCACTC
CGGCTCACCGGACTGACGGCCGAGCCGTTCCTGATGACCCACCCGCCGGTGACCGTCAAC
GTGGACATGGAGATCGACCTGTACGAGAGCGCGGAGGACGGCGGCCTCGCCGGCACCGTG
CTGTACAGCAAGTCCCTCTTCGACCGTGCCACGATCGAGCGGTTCTGTGACGACGTGGTG
GCGGTCGTCTCCACGGCCGCCGCGGATCCCGGACGGCCGGTCTCACAGGTGTGGCAGGGC
CGAGGTCGCGACCAGTGA

[15] C	40..338

[15] A	524..967

[15] PCP	1056..1123

[16] C	1144..1437

[15] C	118..1014

[15] A	1570..2901

[15] PCP	3166..3369

[16] C	3430..4311

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR001242 Condensation domain (Domain) PF00668 Condensation IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) PF00550 PP-binding SSF47336 ACP_like G3DSA:1.10.1200.10 ACP_like PS50075 ACP_DOMAIN IPR010071 Amino acid adenylation domain (Domain) TIGR01733 AA-adenyl-dom IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

first Merid_00020