Naphcy_00050 : CDS information

Location

Organism	Streptomyces arenae
Strain	DSM 40737
Entry name	Naphthocyclinone
Contig	AF098966
Start / Stop / Direction	1,679 / 1,071 / - [in whole cluster] 1,679 / 1,071 / - [in contig]
Location	complement(1071..1679) [in whole cluster] complement(1071..1679) [in contig]
Type	CDS
Length	609 bp (202 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative LuxR family transcriptional regulator
Product (GenBank)	regulator
Gene
Gene (GenBank)	ncnR
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q9X465 PmId [10206788] Isolation and characterization of the naphthocyclinone gene cluster from Streptomyces arenae DSM 40737 and heterologous expression of the polyketide synthase genes. (Gene. , 1999) comment S. coelicolorのactinorhodin PKS由来保存領域でのハイブリによって、putative naphthocyclinone gene clusterを含むcosmid cloneを分離。 NcnR: putative regulatory protein 配列解析のみ。 reguratory protein DnrNとid48%
Related Reference	ACC Q54821 NITE Adria_00100 PmId [7868594] Regulation of daunorubicin production in Streptomyces peucetius by the dnrR2 locus. (J Bacteriol. , 1995) [8892835] The DnrN protein of Streptomyces peucetius, a pseudo-response regulator, is a DNA-binding protein involved in the regulation of daunorubicin biosynthesis. (J Bacteriol. , 1996) [10846224] The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene. (Microbiology. , 2000) [20122958] The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology. (Int J Biol Macromol. , 2010) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 90th, id48%, 7e-45 Streptomyces peucetius_dnrN Regulatory protein [DoBISCUIT]transcriptional activator --- [PMID: 7868594](1995) DnrNはresponse regulatorに配列は似ているが、putative phosphorylation siteの置換(D55E, D55N)をしても活性が完全にはなくならず、近くにHKが見当たらないことからpseudo-response regulatorとも呼ばれている。 dnrN::aphII mutationはdnrIで相補可能。 dnrI::aphII mutantはdnrNで相補できない。よってdrnNがdnrIの転写を活性化するというregulatory cascadeの存在が示唆される。 --- [PMID: 8892835](1996) DnrNをE.coliで過剰発現し、抽出液でgel mobility shift analysis. DnrNは高い親和性で、dnrI promoter領域に特異的に結合する。 wild-type DnrNとputative phosphorylation site置換(D55N)mutantで結合能力に違いがなく、DnrNのリン酸化はDNA bindingに必要ない。 dnrN発現がDNR産生と同時に起こり、DnrNのレベルはDNRを産生しないmutant株で低いので、dnrNとdnrIの発現はDNRによってfeedback調節されると推測される。 --- [PMID: 10846224](2000) dnrO::aphII mutant strainはdaunorubicin (DNR)非産生。このmutant strainにdnrN or dnrI を導入すると生合成可能。 dnrI 発現はDnrNが必要なことがわかっているので、dnrN発現にDnrOが必要ということが示される。 DnrO → dnrN = DnrN → dnrI = DnrI →DNR生合成genes DnrOをE.coliで過剰発現し、抽出液でmobility shift DNA-binding assays。 DnrOが特異的にoverlapping dnrN/dnrO(p1) promoter regionへ結合するので、DnrOはdnrNとdnrOの両方の発現を調節するかもしれない。 --- [PMID: 20122958][PMID: 20529134][PMID: 20158521](2010) dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 DnrOはDnrNと競合してfeedback的にDNRの細胞内濃度を調節している。

Sequence

>putative LuxR family transcriptional regulator [regulator]
MIRVLLAEDMGMVRGALVALMEMEQDLSVVAQVADGREVLSAGLAHRPDVAVIDVGLPGV
DGIEAAAQLHEHLPECRTLMLTGTDQPGTVRRAMDARVFGYLLKDAPPSELAAAIRKVAD
GQRVIDTRLAFTVWQRGDHPLTERESDVLSLASEGAEASEIAAQLHLAVGTVRNYLSSAV
TKLGARNRLDAVRIARDAGWIG

>putative LuxR family transcriptional regulator [regulator]
GTGATTCGCGTCTTGCTCGCCGAGGACATGGGGATGGTGCGAGGGGCGCTGGTGGCGCTG
ATGGAGATGGAGCAGGATCTCAGTGTCGTGGCGCAGGTGGCCGACGGGCGCGAGGTCCTC
TCCGCCGGGCTGGCGCACCGCCCAGACGTCGCCGTGATCGACGTGGGACTTCCCGGGGTG
GACGGCATCGAGGCCGCGGCCCAGTTGCACGAGCACCTGCCGGAGTGCCGGACCCTGATG
CTCACCGGCACGGACCAGCCGGGCACCGTGCGCCGGGCGATGGACGCCAGGGTCTTCGGC
TACCTGCTCAAGGACGCCCCGCCGTCGGAGCTGGCCGCGGCGATCCGCAAGGTGGCGGAC
GGCCAGCGGGTGATCGACACCCGGCTGGCCTTCACCGTATGGCAGCGCGGCGACCATCCG
CTGACCGAGCGGGAGTCCGACGTCCTGTCACTGGCGTCGGAAGGGGCTGAGGCCTCGGAG
ATCGCCGCCCAGCTTCACCTGGCCGTCGGTACCGTCCGGAACTACCTGAGTTCGGCGGTG
ACCAAGCTGGGCGCCCGCAACCGTCTCGACGCGGTGCGGATCGCCCGGGACGCCGGCTGG
ATCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PF00196 GerE SM00421 HTH_LUXR PR00038 HTHLUXR PS00622 HTH_LUXR_1 PS50043 HTH_LUXR_2 IPR001789 Signal transduction response regulator, receiver domain (Domain) SM00448 REC PF00072 Response_reg PS50110 RESPONSE_REGULATORY IPR011006 CheY-like superfamily (Domain) SSF52172 CheY_like IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

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