Naphcy_00050 Naphcy_00050   Naphcy_00070 Naphcy_00070

Naphcy_00060 : CDS information

close this sectionLocation

Organism
StrainDSM 40737
Entry nameNaphthocyclinone
Contig
Start / Stop / Direction2,030 / 2,962 / + [in whole cluster]
2,030 / 2,962 / + [in contig]
Location2030..2962 [in whole cluster]
2030..2962 [in contig]
TypeCDS
Length933 bp (310 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productcyclase/dehydratase
Product (GenBank)cyclase
Gene
Gene (GenBank)ncnE
EC number
Keyword
  • 2nd ring
Note
Note (GenBank)
Reference
ACC
PmId
[10206788] Isolation and characterization of the naphthocyclinone gene cluster from Streptomyces arenae DSM 40737 and heterologous expression of the polyketide synthase genes. (Gene. , 1999)
comment
S. coelicolorのactinorhodin PKS由来保存領域でのハイブリによって、putative naphthocyclinone gene clusterを含むcosmid cloneを分離。

NcnE: cyclase

actIVとid55.2%あり。
ncnE + actKR, PKS, ARO → 3,8 DMAC and aloesaponarin II産生
act KR, PKS, ARO, CYCでも同じもの産生。
よってNcnE = act CYC(actIV gene)

ActIVはC5-C14での2nd ring cyclizationを担う。
Related Reference
ACC
Q93IZ0
NITE
Actino_00220
PmId
[11851466] Genetic Contributions to Understanding Polyketide Synthases. (Chem Rev. , 1997)
comment
Blast 16th, id56%, 1e-81
Streptomyces coelicolor_SCO5091
Cyclase
[DoBICUIT]cyclase/dehydratase_actIV(actIORF5)

--
[Pubmed登録なし]Engineered Biosynthesis of Novel Polyketides: actVII and actIV Genes Encode Aromatase and Cyclase Enzymes, Respectively. J. Am. Chem. Soc., 1994, 116 (24), pp 10855-10859

act minimal PKS, KR(actIII ), actVII, actIV の様々な組み合わせのrecombinantsによって産生されたpolyketides構造を測定。

minimal PKS = SEK4
minimal PKS + act KR = mutactin
minimal PKS + act KR + actVII = SEK34
minimal PKS + act KR + actVII + actIV = DMAC

ActVII は1st carbocyclic ringの2つのdehydrationsを触媒することを担うaromataseとして機能する。
AcyIV は2つめのringを形成するための分子内aldol縮合を触媒するcyclase(aldolase)である。

ActVII homologsは、gra, fren, tcm, gris生合成clusterにある。
ActIV homologsは、gra, fren, gris生合成clusterにある。

--
[PMID:11851466](1997)
上記PMIDなし論文がRef採用されているレビュー。

close this sectionSequence

selected fasta
>cyclase/dehydratase [cyclase]
MTDERRQDRTAPDDAWLEEVADGVFAYVQPDGGWCLNNAGLVVSDGRAALVDTAATETRA
RRLREAVRGVTAAPPGVLVNTHFHGDHTFGNFVFPEALVVGHERTRSEALAAGLHLTGLW
PDVRWGALELAPPALTYRDGVTLHVGDVRVEVLHPGPAHTTDDSVVWLPEQRVLFTGDIV
MPGVTPFCAMGSVSGSLAVLDRLRALGARTVVPGHGPVAGPEVFDATESYLRWVRATARR
GLADRLTPMQVARACDLGEFAGLRDSERLLPNLRRAYAEEQGAAPGAPLDIGELFAEMIE
FHGRLPTCRA
selected fasta
>cyclase/dehydratase [cyclase]
GTGACCGACGAACGGCGGCAGGACCGCACGGCCCCGGACGACGCGTGGCTGGAGGAGGTG
GCGGACGGCGTCTTCGCCTACGTCCAGCCGGACGGCGGCTGGTGCCTGAACAACGCCGGT
CTCGTCGTGTCCGACGGCCGGGCGGCGCTCGTCGACACCGCCGCGACCGAGACCCGGGCC
CGCCGCCTGCGGGAGGCCGTGCGCGGGGTGACGGCGGCACCCCCCGGCGTCCTGGTCAAC
ACCCACTTCCACGGCGACCACACCTTCGGCAACTTCGTCTTCCCCGAAGCGCTGGTCGTC
GGCCACGAACGGACCCGCAGCGAGGCGCTGGCGGCCGGCCTGCACCTCACCGGCCTGTGG
CCCGACGTGCGCTGGGGCGCCCTCGAACTGGCGCCCCCGGCCCTCACCTACCGCGACGGG
GTGACCCTGCACGTGGGAGACGTGCGCGTCGAGGTGCTGCACCCGGGCCCGGCGCACACG
ACGGACGACTCGGTCGTCTGGCTGCCCGAGCAGAGGGTGCTGTTCACCGGGGACATCGTC
ATGCCGGGCGTCACGCCGTTCTGCGCGATGGGATCGGTCAGCGGATCCCTCGCCGTCCTC
GACCGGCTGCGCGCACTGGGCGCCCGTACCGTGGTGCCGGGCCACGGCCCGGTCGCGGGC
CCCGAGGTGTTCGACGCCACCGAGAGCTACCTGCGGTGGGTCCGGGCGACGGCACGGCGC
GGCCTGGCCGACCGGCTCACCCCCATGCAGGTCGCCCGCGCCTGCGACCTGGGCGAGTTC
GCCGGGCTCCGCGACTCCGAACGGCTACTGCCGAACCTGCGCCGCGCCTACGCGGAGGAG
CAGGGTGCGGCCCCCGGGGCGCCGCTGGACATAGGGGAGCTGTTCGCCGAGATGATCGAG
TTTCACGGCCGGCTGCCGACCTGCCGGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001279 Beta-lactamase-like (Domain)
[33-215] 4.9e-24 PF00753
PF00753   Lactamase_B
[36-215] 2.1000026783403e-34 SM00849
SM00849   Lactamase_B
SignalP
[1-35] 0.036 Signal
Eukaryota   
TMHMM No significant hit
Naphcy_00050 Naphcy_00050   Naphcy_00070 Naphcy_00070
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