Ncarz_00090 Ncarz_00090   Ncarz_00110 Ncarz_00110

Ncarz_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 15944
Entry nameNeocarzinostatin
Contig
Start / Stop / Direction27,188 / 26,475 / - [in whole cluster]
27,188 / 26,475 / - [in contig]
Locationcomplement(26475..27188) [in whole cluster]
complement(26475..27188) [in contig]
TypeCDS
Length714 bp (237 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative nucleotidyltransferase
Product (GenBank)NDP-hexose synthetase
GenencsC
Gene (GenBank)
EC number2.7.7.-
Keyword
  • deoxy aminosugar
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005)
comment
Neocarzinostatin生合成遺伝子クラスターの報告。

ncsC: dNDP-mannose synthase

相同性より機能付けしている。
dNDP-D-mannose synthasesに対するNcsCの配列相同性をもとに、sugar moietyの生合成はdNDP-D-mannoseから始まると提案している。
C-2ヒドロキシル基はNeocarzinostatin(NCS)のdeoxy aminosugar moietyでは最終的に欠損しているためD-glucose-1-phosphateから合成が始まり、NcsCがdNDP-D-glucose synthaseであることは除外出来ない。しかし、NcsC1のC末領域が既知のNGDHs(dNDP-D-glucose 4,6-dehydratase)と相同性が乏しくglucose 4,6-dehydratase enzymesの配列を用いたプライマーでNcsC1の増幅が出来ないことから、おそらくdNDP-D-mannoseがNcsC1の基質であると推定している。
Family/Domain
FD
IPR005835
comment
[IPR005835] Nucleotidyl transferase (Domain)

close this sectionSequence

selected fasta
>putative nucleotidyltransferase [NDP-hexose synthetase]
MHAVILAGGKGTRLRPYTSRLPKPLVPIGDEFSILEIVLSQLSQRGFDTVTLAIGYLGHL
IRSFVGDGSRWNMHITYASEDTPRGTIGPVLGILDQLPEHFLVMNGDILTDLDYADVLAH
HTRGEAPLTVATCTREVRIDFGVLTTTSNRVVHFVEKPTVDYRVSMGVYGLSRTTLETYP
SHMPFGFDDLVLDLLARGVQPAEYPFDGYWLDIGRPDDYDRANAEFDSIKGTLLKGA
selected fasta
>putative nucleotidyltransferase [NDP-hexose synthetase]
ATGCACGCAGTGATCCTGGCGGGCGGCAAGGGGACGAGGCTGCGGCCGTACACCAGCCGG
CTGCCCAAACCCCTGGTGCCGATCGGTGACGAGTTCTCGATCCTGGAGATCGTCCTGAGC
CAGCTCTCCCAGCGGGGATTCGACACGGTGACGCTCGCCATCGGCTACCTCGGCCACCTG
ATCCGTTCCTTCGTGGGCGACGGCAGCCGATGGAACATGCACATCACCTACGCCTCGGAG
GACACGCCCCGGGGGACGATCGGCCCGGTCCTGGGCATCCTCGACCAACTGCCCGAACAC
TTCCTGGTCATGAACGGTGACATCCTCACCGACCTGGACTACGCCGATGTGCTGGCCCAT
CACACCAGGGGTGAGGCGCCGCTGACGGTGGCGACGTGCACGCGCGAGGTGCGTATCGAC
TTCGGCGTGCTGACGACGACCAGCAACCGGGTGGTCCACTTCGTGGAGAAGCCGACGGTC
GACTACCGGGTCAGCATGGGGGTCTACGGCCTGAGCCGCACCACCCTCGAAACCTATCCG
TCCCACATGCCGTTCGGCTTCGACGACCTGGTCCTCGACCTGCTGGCCCGCGGTGTCCAG
CCGGCGGAGTACCCCTTCGACGGGTACTGGCTGGACATCGGCCGCCCTGACGACTACGAC
CGCGCCAACGCCGAGTTCGACTCGATCAAGGGGACCCTGCTCAAGGGGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[3-225] 5e-36 PF00483
PF00483   NTP_transferase
SignalP No significant hit
TMHMM No significant hit
Ncarz_00090 Ncarz_00090   Ncarz_00110 Ncarz_00110
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