Ncarz_00170 Ncarz_00170   Ncarz_00190 Ncarz_00190

Ncarz_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 15944
Entry nameNeocarzinostatin
Contig
Start / Stop / Direction36,622 / 35,390 / - [in whole cluster]
36,622 / 35,390 / - [in contig]
Locationcomplement(35390..36622) [in whole cluster]
complement(35390..36622) [in contig]
TypeCDS
Length1,233 bp (410 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)cytochrome P-450
GenencsB3
Gene (GenBank)
EC number
Keyword
  • naphthoic acid
  • C-7 hydroxylate
Note
Note (GenBank)
Reference
PmId
[15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005)
[20735485] In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore. (FEMS Microbiol Lett. , 2010)
comment
[PMID:15797213]
Neocarzinostatin生合成遺伝子クラスターの報告。

ncsB3: P-450 hydroxylase

[PMID:20735485]
NcsB3の機能解析報告。

ncsB,B1,B3をS.lividans TK24で発現。これらの産物をHPLCにて解析した結果、ncsB3はnaphthoic acidのC-7 positionでのhydroxylationを触媒するcytochrome P450であると示した。

close this sectionSequence

selected fasta
>cytochrome P450 [cytochrome P-450]
MCPYRLDPEGADTHGETARLREQGPIARVELQDGVLAWSVHDYAVAKQIMADERFSKNPR
KNWPAYINGEISNGWPLITWVAMDTMATQDGADHARLRKLLLKAFTERRVESMRPHIEKT
VKELLDNMAAKADDEIVDIKEMFHAELPTRLMCDLFGVPEERRAEVLAGGHKNIDTRISS
EAAEANLGQWQEAISDLVEYKRHHPGDDLTSALIEARDEGSRLSDSELIGTLHLLLGAGS
ETLVNALAHSSLALLVDADLRKKVTSGEIPWVNVWEETLRVESPVAHLPFRYATEDFEIG
GVKISKGDPLLVDFAGIGRDPAVHSDAPDEFDALRPDKTHLSFGHGVHYCLGARLAKHAW
MIGIPALFERFPDMELAVRRDELKGQGSFVVNGHASLPVHLKGRAAALAR
selected fasta
>cytochrome P450 [cytochrome P-450]
GTGTGCCCTTACCGTCTTGACCCCGAGGGCGCCGACACCCACGGGGAGACCGCTCGTCTG
CGCGAGCAGGGCCCGATCGCACGGGTCGAACTCCAGGACGGCGTGCTTGCGTGGTCGGTG
CACGACTACGCGGTCGCCAAGCAGATCATGGCCGACGAGCGCTTCTCCAAGAACCCCCGC
AAGAACTGGCCCGCGTACATCAACGGGGAGATATCGAACGGCTGGCCCCTGATCACCTGG
GTGGCCATGGACACCATGGCCACCCAGGACGGCGCCGACCACGCACGTCTGCGCAAGCTG
CTCCTGAAGGCCTTCACCGAGCGTCGTGTCGAGTCCATGAGGCCGCACATCGAAAAGACG
GTGAAGGAACTCCTCGACAACATGGCCGCCAAGGCCGACGACGAGATCGTGGACATCAAG
GAGATGTTCCACGCCGAGCTGCCCACGCGGCTGATGTGCGACCTCTTCGGTGTGCCGGAG
GAGAGGCGCGCCGAGGTGCTGGCGGGGGGACACAAGAACATCGACACCCGGATCTCCTCC
GAGGCGGCGGAAGCCAACCTCGGGCAGTGGCAGGAAGCCATCTCCGATCTCGTCGAGTAC
AAGCGTCATCACCCCGGTGACGACCTCACGAGTGCACTGATCGAGGCCAGGGACGAGGGT
TCGCGGCTGAGTGACTCGGAGCTGATCGGCACGCTGCACCTGCTGCTCGGCGCCGGCTCC
GAGACTCTGGTGAATGCCCTCGCCCACTCCTCCCTGGCCCTGCTCGTCGACGCCGACCTG
CGCAAGAAGGTGACCTCTGGCGAGATCCCCTGGGTGAACGTCTGGGAGGAGACGCTGCGA
GTGGAGTCGCCCGTGGCACACCTGCCCTTCCGCTACGCGACGGAGGACTTCGAGATCGGC
GGTGTGAAGATCTCGAAGGGCGATCCCCTGCTGGTCGACTTCGCGGGCATCGGCCGGGAC
CCTGCCGTACACAGCGATGCCCCCGACGAGTTTGACGCTCTCCGGCCCGACAAGACGCAC
CTCTCGTTCGGCCACGGCGTCCACTACTGCCTCGGCGCGCGGCTCGCGAAGCACGCCTGG
ATGATCGGCATCCCCGCATTGTTCGAGCGCTTCCCCGACATGGAACTCGCGGTCCGCCGT
GACGAACTCAAGGGGCAGGGAAGCTTCGTGGTCAACGGCCATGCCAGTCTGCCGGTGCAC
CTGAAGGGCCGGGCCGCGGCTCTCGCCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[10-404] 2.59999999999995e-94 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[274-371] 1.8e-14 PF00067
PF00067   p450
[11-404] 2.1000026783403e-87 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[89-100] 1.70000295590054e-30 PR00359 [138-154] 1.70000295590054e-30 PR00359 [155-170] 1.70000295590054e-30 PR00359 [192-214] 1.70000295590054e-30 PR00359 [273-284] 1.70000295590054e-30 PR00359 [291-318] 1.70000295590054e-30 PR00359 [341-350] 1.70000295590054e-30 PR00359 [350-361] 1.70000295590054e-30 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[343-352] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Ncarz_00170 Ncarz_00170   Ncarz_00190 Ncarz_00190
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