Nogl_00110 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AF323753
Start / Stop / Direction	12,635 / 12,321 / - [in whole cluster] 12,635 / 12,321 / - [in contig]
Location	complement(12321..12635) [in whole cluster] complement(12321..12635) [in contig]
Type	CDS
Length	315 bp (104 aa)

Annotation

Category	3.4 other modification
Product	C-4'' epimerase(fragment)
Product (GenBank)	SnoN
Gene
Gene (GenBank)	snoN
EC number
Keyword	nogalamine
Note
Note (GenBank)
Reference	ACC Q9EYI0 PmId [11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001) [27114534] Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway. (Proc Natl Acad Sci U S A. , 2016) comment [PMID: 11683270](2001) S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。 nogalamycin生合成に関する20ORFsが明らかになった。 snoN(293aa): Unknown closest homolog SnoK DBに有意な類似性のあるタンパクなし。 snoNを含むplasmid(pSYE63d)を異種mutantに導入しても、産物特性に変化はなかった。 --- [PMID: 27114534](2016) nogalamycinの生合成において、SnoNはC4''炭素でのepimerization stepを触媒する。

Sequence

>C-4'' epimerase(fragment) [SnoN]
MWMPRVTVPLRAGDCTFHHARTVHSAGANSTDEPRLSTSAVYMDATAAYRPTGIAFLDDL
PGTGADPLREGAPLTGDRFPLLRRPQTRQPAPARYPLPQQGHGS

>C-4'' epimerase(fragment) [SnoN]
ATCTGGATGCCGCGGGTCACGGTGCCACTGCGGGCCGGCGACTGCACCTTCCACCACGCC
CGCACCGTGCACAGCGCCGGGGCGAACTCGACCGACGAGCCCCGCTTGTCGACCTCCGCC
GTCTACATGGACGCCACCGCCGCCTACCGGCCGACCGGCATCGCGTTTCTCGACGACCTC
CCCGGCACCGGAGCCGACCCGCTGCGCGAGGGCGCCCCCCTCACCGGCGACCGCTTCCCC
CTGCTGCGCCGACCGCAGACCCGACAGCCCGCCCCCGCCCGGTATCCCCTTCCACAGCAA
GGACACGGATCTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008775 Phytanoyl-CoA dioxygenase (Family) PF05721 PhyH
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Nogl_00100 Nogl_00120