Nogl_00090 Nogl_00090   Nogl_00110 Nogl_00110

Nogl_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 27451 (=NBRC 13445)
Entry nameNogalamycin
Contig
Start / Stop / Direction12,324 / 11,110 / - [in whole cluster]
12,324 / 11,110 / - [in contig]
Locationcomplement(11110..12324) [in whole cluster]
complement(11110..12324) [in contig]
TypeCDS
Length1,215 bp (404 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)
GenesnoT
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
PmId
[11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001)
comment
相当するUniProt entryなし。

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S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。
nogalamycin生合成に関する20ORFsが明らかになった。

snoT: Unknown

snoTを含むplasmid(pSYE63, pSYE63d)を異種mutantに導入しても、産物特性に変化はなかった。
Related Reference
ACC
P0ABR5
PmId
[9603882] Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. (J Bacteriol. , 1998)
[9205837] ( , )
comment
Blast 300th, id32%, 2e-24
N末部分同士のhit, C末alignment悪い。
E.coli_hcaE(hcaA, hcaA1 etc.)
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha(EC 1.14.12.19)
synonym: Digoxigenin subunit alpha

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UniProt function(ref.5=[PMID: 9603882](1998));
multicomponent 3-phenylpropionate dioxygenaseの一部。

3-phenylpropionic acid (PP) → 3-phenylpropionate-dihydrodiol (PP-dihydrodiol)
cinnamic acid (CI) → cinnamic acid-dihydrodiol (CI-dihydrodiol)

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[PMID: 9205837](1997)
3-phenylpropionic acid (PP)の初期代謝に関与するdioxygenolytic pathwayをコードする遺伝子群として、 hca clusterを同定している。
hcaA1A2CBDのうち、hcaA1A2CDが3-phenylpropionate dioxygenaseをコードし、hcaBがcis-dihydrodiol dehydrogenaseをコードすることが示唆されている。また、hcaTがpermease, hcaRがregulatorをコードすることが推測されている。
Family/Domain
FD
IPR017941
comment
[IPR017941] Rieske [2Fe-2S] iron-sulphur domain (Domain)
function; oxidoreductase activity, 2 iron, 2 sulfur cluster binding

close this sectionSequence

selected fasta
>putative oxidoreductase
MTLHQTGPAAPRPDSSTTPPLSPETADLLAELGGYLVPDPPALSLPPRAFASGELYELER
EKVFGRSWLLVAHTDQLAGPGAYVALTVAGEPVVITRGDDGALHAMSPICRHRMMPVVEP
GTGRTDRFTCPYHLWRYGLDGRLIGATFMRRNEAFDPAGCRLPQFAVEEWHGFVFVNLDP
AAEALAPHLARIESDLVNYRLDDMVLAGEWAEEWHCNWKIAVENAHENYHVMGFHPETLE
PSTPGRSDTDLRDDTPWALRFRIPFTEPQQPSLLSLTEDEKAHLYDFFVFPTGSLAAAGE
AVIWLSMLPLSLDRTQVRGGMLVPAARVKDLGEAERKEMRQGIESYSAMVNAEDRRGLEA
VQRSVGSRFAARGHLSPKEPAVVSFYRNLARALLRGDDEWPGAL
selected fasta
>putative oxidoreductase
TTGACCCTCCATCAGACCGGCCCGGCGGCCCCGCGGCCGGACTCCTCCACCACACCCCCT
CTGTCGCCGGAGACCGCGGACCTGCTCGCCGAACTCGGTGGCTACCTGGTCCCGGACCCG
CCCGCCCTCAGCCTGCCGCCCCGGGCGTTCGCCTCCGGTGAGCTGTACGAGCTGGAGCGG
GAGAAGGTCTTCGGCCGTTCCTGGCTTCTCGTCGCGCACACCGACCAACTCGCCGGCCCC
GGTGCCTACGTCGCCCTCACCGTCGCGGGCGAACCCGTCGTCATCACGCGCGGCGACGAC
GGCGCGCTGCACGCGATGTCACCGATCTGCCGCCACCGCATGATGCCGGTCGTCGAACCG
GGCACCGGCCGCACCGACCGGTTCACCTGCCCGTACCACCTGTGGCGCTACGGACTGGAC
GGCCGGTTGATCGGCGCCACCTTCATGCGGCGCAACGAGGCGTTCGACCCCGCCGGCTGC
CGACTGCCGCAGTTCGCCGTGGAAGAGTGGCACGGGTTCGTCTTCGTCAACCTCGACCCC
GCGGCGGAAGCCCTGGCTCCGCACCTCGCCCGAATCGAAAGCGACCTGGTCAACTACCGC
CTGGACGACATGGTACTGGCCGGGGAATGGGCCGAGGAGTGGCACTGCAACTGGAAGATC
GCCGTGGAGAACGCGCACGAGAACTACCACGTGATGGGCTTCCACCCCGAGACCCTGGAA
CCGTCCACCCCGGGCCGCTCCGACACGGATCTGCGCGACGACACGCCGTGGGCGCTGCGT
TTCCGCATCCCGTTCACCGAGCCCCAGCAGCCGTCCCTCCTCTCCCTGACCGAGGACGAG
AAGGCTCACCTGTACGACTTCTTCGTCTTCCCCACCGGGAGCCTCGCCGCCGCCGGGGAG
GCGGTGATCTGGCTGAGCATGCTCCCGTTGTCCCTCGACCGCACCCAGGTCCGCGGGGGC
ATGCTGGTCCCCGCCGCCCGGGTCAAGGACCTCGGTGAGGCCGAACGGAAGGAGATGCGG
CAGGGCATCGAGTCGTACTCCGCGATGGTCAACGCGGAGGACCGGCGCGGCCTGGAGGCC
GTGCAGCGGTCCGTGGGCTCCCGGTTCGCCGCGCGCGGCCACCTCAGCCCCAAGGAGCCG
GCCGTCGTCTCCTTCTACCGGAACCTGGCGCGCGCCCTGCTGCGTGGCGACGACGAGTGG
CCCGGCGCCCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001663 Aromatic-ring-hydroxylating dioxygenase, alpha subunit (Domain)
[52-77] 6e-26 PR00090 [82-97] 6e-26 PR00090 [103-123] 6e-26 PR00090 [124-144] 6e-26 PR00090 [165-191] 6e-26 PR00090 [202-224] 6e-26 PR00090
PR00090   RNGDIOXGNASE
IPR015879 Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domain (Domain)
[201-393] 1.3e-29 PF00848
PF00848   Ring_hydroxyl_A
IPR017941 Rieske [2Fe-2S] iron-sulphur domain (Domain)
[68-176] 33.154 PS51296
PS51296   RIESKE
[66-175] 1.3e-33 G3DSA:2.102.10.10
G3DSA:2.102.10.10   no description
[67-161] 6.9e-18 PF00355
PF00355   Rieske
[28-181] 1.7e-46 SSF50022
SSF50022   ISP domain
SignalP No significant hit
TMHMM No significant hit
Nogl_00090 Nogl_00090   Nogl_00110 Nogl_00110
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