Nogl_00190 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AF187532
Start / Stop / Direction	19,711 / 18,908 / - [in whole cluster] 7,074 / 6,271 / - [in contig]
Location	complement(18908..19711) [in whole cluster] complement(6271..7074) [in contig]
Type	CDS
Length	804 bp (267 aa)

Annotation

Category	3.4 other modification
Product	cyclase
Product (GenBank)	SnoK
Gene
Gene (GenBank)	snoK
EC number
Keyword	nogalamine C-5'' and C-2
Note
Note (GenBank)
Reference	ACC Q9RN60 PmId [11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001) [27114534] Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway. (Proc Natl Acad Sci U S A. , 2016) comment [PMID: 11683270](2001) S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。 nogalamycin生合成に関する20ORFsが明らかになった。 snoK: Unknown closest homolog SnoN DBに有意な類似性のあるタンパクなし。 --- [PMID: 27114534](2016) nogalamycinの生合成において、SnoKはC5''-C2 carbocyclizationを触媒する。

Sequence

>cyclase [SnoK]
MPDPGGPTTAENLSKEAVRFYREQGYVHIPRVLSETEVTAFRAACEEVLEKEGREIWGAG
EDEVQVHYVAQAWQKHPELRSLVLHPEISGIALRLAGAPLRVYSSDILVKEPKRTLPTLV
HDDETGLPLNELSATLTAWIALTDVPVERGCMSYVPGSHLRAREDRQEHMTSFAEFRDLA
DVWPDYPWQPRVAVPVRAGDVVFHHCRTVHMAEANTSDSVRMAHGVVYMDADATYRPGVQ
DGHLSRLSPGDPLEGELFPLVTAGTRQ

>cyclase [SnoK]
ATGCCGGATCCCGGCGGCCCGACCACGGCCGAGAACCTGTCGAAGGAGGCTGTCCGCTTC
TACCGCGAGCAGGGTTACGTGCACATCCCGCGCGTCCTGTCGGAGACGGAGGTGACCGCC
TTCCGGGCCGCCTGTGAGGAGGTCCTGGAGAAGGAGGGCCGCGAGATCTGGGGCGCGGGC
GAGGACGAGGTGCAGGTCCACTACGTCGCCCAGGCCTGGCAGAAGCACCCGGAGTTGCGC
TCCCTCGTGCTGCACCCGGAGATCTCCGGCATCGCCCTGCGGCTGGCCGGCGCGCCCCTG
CGGGTCTACAGCAGCGACATCCTGGTCAAGGAGCCCAAGCGCACCCTGCCCACCCTGGTC
CACGACGACGAGACGGGACTGCCGCTGAACGAGCTGAGTGCCACGCTGACGGCCTGGATC
GCGCTGACGGACGTACCCGTCGAACGCGGCTGCATGAGCTACGTGCCGGGCTCCCATCTC
AGGGCCCGCGAGGACCGGCAGGAGCACATGACCAGCTTCGCCGAGTTCCGGGACCTCGCG
GACGTGTGGCCCGATTACCCGTGGCAGCCGCGCGTCGCCGTGCCCGTCCGCGCCGGAGAC
GTCGTGTTCCACCATTGCCGTACCGTCCACATGGCCGAAGCCAACACCAGCGACTCGGTC
CGCATGGCGCATGGCGTCGTCTACATGGACGCGGACGCCACCTACCGGCCGGGCGTCCAG
GACGGCCACCTGTCCCGCCTGTCGCCGGGAGATCCACTCGAAGGCGAGCTGTTCCCCCTG
GTCACGGCAGGCACACGGCAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008775 Phytanoyl-CoA dioxygenase (Family) PF05721 PhyH
SignalP	No significant hit
TMHMM	No significant hit

Nogl_00180 Nogl_00200